65 results about "Soyauxia" patented technology

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

The invention relates to the technical field of agricultural biology, discloses a soybean shade-tolerant gene GmUGT73C2 as well as a molecular marker and application thereof, and is characterized in that the gene GmUGT73C2 which has a remarkable regulation effect on the plant height and shade tolerance of soybeans is obtained through screening and verification; the plant height of the wild soybean is remarkably reduced and the shade tolerance of the soybean is improved by overexpression of the gene in the soybean plant. A novel gene and a novel method are provided for construction of dwarf and shade-tolerant soybean plants. Meanwhile, an SNP locus corresponding to the gene is found, a corresponding molecular marker is developed, and an efficient screening tool is provided for construction of dwarf and shade-tolerant plants.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance and methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenes and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

The invention belongs to the technical field of molecular breeding, and particularly relates to application of an E1-SOC1 gene model in cultivation of wide-adaptability soybean varieties. Research finds that three genes (E1 and homologous genes E1La and E1Lb) of an E1 family and two genes (SOC1a and SOC1b) of an SOC1 family are knocked out from soybeans at the same time by utilizing a gene editing technology, and the obtained homozygous five mutants show wide adaptability when planted under field conditions of different latitude regions; and the method has certain wide adaptability in the aspects of plant height, section number, flowering period, mature period and yield. It is indicated that the E1-SOC1 gene module (E1, E1La, E1Lb, SOC1a and SOC1b genes) is expected to be applied to molecular design breeding for breeding wide-adaptability soybean models, and has important application prospects in soybean molecular breeding research.

The present invention relates to a method for controlling pests of a modified plant, in particular a soybean plant, comprising the step of contacting the plant, a part thereof, a propagation material thereof, a pest, a food supply, a habitat or a breeding ground thereof with one or more compounds of formula I, wherein the variables are defined as described in the specification.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR39 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The invention relates to the technical field of agricultural biology, in particular to SNP related to soybean plant height and a KASP primer and application thereof, S1855999953 remarkably related to soybean plant height is located at the 55999953bp position of the No.18 chromosome of a soybean genome Wm82. A4. V1, the site polymorphism is G or A, and both the GG genotype and the AA genotype can provide technical support for molecular marker-assisted breeding of soybean plant height characters. The KASP molecular marker is further developed, specific distinguishing and detection can be directly conducted on G or A basic groups of SNP mutation sites, the soybean plant height can be rapidly and accurately identified through the marker, the influence of the environment on soybean plant height character identification is reduced, the breeding period is shortened, and selection and improvement of soybean plant height characters are accelerated.

Methods and compositions are provided for modifying the flowering time and / or maturity time of soybean plants to enable them to be cultivated in a variety of geographical locations having different day lengths. Modified soybean plants are disclosed comprising non-natural mutant alleles at the E1, E2, E3, E4, E1La and / or E1Lb locus. The modified plants have a shorter flowering and / or maturity time than control plants.

The application discloses a soybean plant height related InDel molecular marker and application thereof, relates to the technical field of biological molecule detection.The nucleotide sequence of the InDel molecular marker is shown as SEQ ID NO.4 or SEQ ID NO.5.The InDel molecular marker developed by the application can realize efficient, accurate and early identification of soybean plant height.The InDel molecular marker has stable polymorphism, high amplification efficiency of the matching specific primer, clear bands, good repeatability and clear typing.It is verified that the length difference of the amplification product is significantly related to the plant height phenotype, high-stalk and dwarf individuals can be accurately distinguished, and the genotype detection result is highly consistent with the field phenotype.The application provides a stable and reliable molecular tool for genetic improvement of soybean plant height and rapid cultivation of high-yield and lodging-resistant materials.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR24 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The invention belongs to the field of plant genes, and relates to application and a method of a GmTOC1a gene or protein in regulation and control of soybean flowering. The application of the soybean biological clock gene GmTOC1a in flowering control is found, two homologous copies GmTOC1a1 (Glyma. 05G025000) and GmTOC1a2 (Glyma. 17G102200) of the GmTOC1a gene are directionally knocked out through a CRISPR / Cas9 gene editing technology, a soybean mutant with the GmTOC1a gene mutated is obtained, research finds that the mutant shortens the growth period of soybeans and advances the flowering time, and it is indicated that the GmTOC1a inhibits soybean flowering. Therefore, the GmTOC1a gene can be applied to change or expand the cultivation regionalism of soybeans, so that the soybeans can be sown at different latitudes, the planting region of the soybeans is expanded, and a theoretical basis and genetic resources are provided for breeding good varieties of the soybeans.

The disclosure relates to novel soybean plants, plant cells, plant parts, and nucleotide sequences in soybean plants comprising (i) a mutated endogenous AIP10a gene promoter and / or AIP10a gene 5' untranslated region (5' UTR), and / or (ii) a mutated endogenous AIP10b gene promoter and / or AIP10b gene 5' UTR, along with methods of using and making the same.

This invention relates to a GmSPL3b gene SNP marker and its application, belonging to the fields of plant molecular biology and crop genetics and breeding technology. The SNP marker is used for molecular detection of soybean flowering and maturity stages. The SNP is a single nucleotide mutation at position 94 and / or position 116 of the first exon of the soybean GmSPL3b gene, forming a nucleotide sequence as shown in SEQ ID NO. 1 and / or SEQ ID NO. 2; the polymorphism of the single nucleotide mutation is A / G polymorphism. This invention enables precise genotyping at the seedling stage, efficiently breeding early-maturing soybean varieties adapted to high-latitude regions. It can directly serve as a precise target for gene editing, providing a clear basis for molecular design breeding. It allows for rapid screening of ideal genotypes in early generations, reducing field workload, shortening the breeding cycle, and accelerating the selection of new varieties.

Provided are a nucleic acid sequence for detecting a Glycine max plant DBN8205 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof. The Glycine max plant DBN8205 has good resistance to insects of Lepidoptera order and good tolerance to glufosinate herbicides, and therefore the yield is not affected. By utilizing the detection method, it is possible to accurately and quickly identify whether a biological sample contains a DNA molecule of a transgenic Glycine max event DBN8205.

The present disclosure provides compositions and methods for making transgenic plants expressing a resistant Lr34 protein that exhibit increased resistance to fungal diseases, and transgenic plants produced by these methods, and plant parts thereof. The present disclosure provides transgenic plants, and plant parts thereof, that are resistant or immune, or have reduced susceptibility, to Asian Soybean Rust (ASR) and other fungal pathogens, while demonstrating minimized or reduced undesirable off-types. The present disclosure provides effective transgenic expression of Lr34 in soybean varieties to reduce the need for one or more fungicide applications, and / or protecting against fungal pathogens developing resistance to both the chemical control agents and other native or engineered disease resistance genes.

This invention discloses the use of an insecticidal protein, specifically a method and its application for controlling lepidopteran pests. The method includes contacting the lepidopteran pests with the insecticidal protein, or introducing a nucleic acid molecule encoding the insecticidal protein into a plant, causing the lepidopteran pests to feed on the plant. The insecticidal protein contains the amino acid sequence shown in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:20. In this invention, the Cry1Da1 proteins BD1-002, BD1-018, BD1-019, BD1-021, BD1-022, and BD1-022S exhibit good insecticidal activity against the soybean pest, the silver-striped armyworm.

The invention provides a soybean single plant field phenotype continuous time-space dynamic analysis method and system. The problems that in field crop phenotype data extraction, group phenotype evaluation neglects single plant heterogeneity, canopy closure restricts single plant segmentation implementation, and unmanned aerial vehicle phenotype data accuracy defects exist are solved. The method comprises the following steps: carrying out whole-growth-period data acquisition through an unmanned aerial vehicle carrying multiple sensors, and carrying out multi-source space-time registration based on ground mark points; identifying a single plant from the seedling stage image and segmenting to obtain an independent single plant image; visible light, multispectral and laser radar data are fused, and the canopy height, the coverage degree and the normalized vegetation index of a single plant are extracted; and a multi-stage evaluation system is constructed by calculating a variable coefficient of phenotypic parameters of single plants in the group, so that the analysis from the heterogeneity of the single plants to the dynamic quantitative evaluation of the growth uniformity of the group is realized.

The application discloses application of 2,6,10-trimethyltridecane as a component of a piezodorus guildinii trapping agent. The inventors find that 2,6,10-trimethyltridecane has strong attraction to piezodorus guildinii, a major soybean pest, and the compound can be used to trap and control the quantity of piezodorus guildinii in a field, so as to achieve the purpose of green prevention and control. When the compound is combined with pheromones, the trapping efficiency can be obviously improved, and the number of mis-trapped natural enemy insects can be reduced, so that the compound has great application prospect.

The invention discloses a plant immune receptor LC-BAK1 and application thereof in plant epidemic disease resistance, and belongs to the technical field of plant disease control. The invention discovers and solves the technical obstacle of constitutive cell death caused by direct fusion of LC and BAK1 for the first time. By introducing regulation and control structural domains such as TM3, the receptor is started only when pathogenic bacteria exist, and the application safety is extremely high. The TM3-LC-BAK1 receptor is specifically activated by the PsAvh85 to trigger a potent immune response including defensive gene up-regulation and active oxygen outbreak, so that the invasion of phytophthora sojae can be effectively resisted.

The disclosure relates to novel soybean plants, soybean plant parts, soybean plant cells, and nucleotide sequences in soybean plants comprising an endogenous gene or fragment thereof containing a deletion of one or more nucleotides between a transcriptional enhancer and a promoter of endogenous target soybean gene sequences to upregulate expression of the endogenous target soybean genes, along with methods of using and making the same.

The disclosure relates to novel soybean plants, plant parts, and nucleotide sequences in soybean plants comprising: (i) a loss-of-function allele in the endogenous JAG1 gene and a loss-of-function allele in the endogenous JAG2 gene, where at least one of the loss-of-function alleles of the endogenous JAG1 or JAG2 genes is a hypomorphic allele of the JAG1 gene or JAG2 gene; and (ii) a loss-of-function allele in the endogenous BS1 gene and / or a loss-of-function allele in the endogenous BS2 gene, along with methods of using and making the same.

The invention belongs to the technical field of pesticide weeding, and discloses a clomazone-containing weeding composition and application thereof.The weeding composition comprises pyroxasulfone serving as an active ingredient A, clomazone serving as an active ingredient B and prometryn serving as an active ingredient C. The mass ratio of the active ingredient A to the active ingredient B to the active ingredient C is (1-5): (1-60): (1-60). The weeding composition disclosed by the invention has a remarkable synergistic effect on weeds in soybean or corn fields, effectively reduces the pesticide application frequency, saves the weeding cost, delays the drug resistance of the weeds, and is beneficial to controlling rapid replacement of dominant species of field weed communities and maintaining agricultural ecological balance.

The application discloses a soybean low-temperature-resistant transcription factor gene GmBZR4 and application thereof, and belongs to the technical field of plant genetic engineering and molecular breeding. GmBZR4 The nucleotide sequence of the gene is shown as SEQ ID NO. 1. GmBZR4 The application clones a gene in a soybean variety Williams 82, constructs an overexpression vector, and transforms the vector into Arabidopsis thaliana, and through low-temperature-resistant phenotype identification, it is proved that overexpression of the gene can significantly improve the low-temperature-resistant adaptability of Arabidopsis thaliana at the seedling stage and the germination stage. GmBZR4 The survival rate of the unacclimatized overexpression strain after treatment at-8 DEG C is up to 81% (7% for the wild type), and the germination speed is significantly faster than that of the wild type under a low-temperature environment. GmBZR4 The gene provided by the application can be used as a target gene resource and applied to the cultivation of low-temperature-resistant plant varieties, and especially provides key technical support for soybean low-temperature-resistant molecular breeding.

The present application relates to the field of crop molecular biology technology, and particularly relates to a SNP marker for soybean flowering period phenotype identification and application thereof. The present application provides a SNP marker for soybean flowering period phenotype identification, which is located at the 12087053th position on the chromosome 2 of soybean, and the deoxynucleotide is T or A. The present application detects the genotypes of the 12087053th position on the chromosome 02 of soybean in dozens of sample materials by using whole genome resequencing method, and the results show that the average flowering time of the material carrying TT genotype is significantly earlier than the flowering time of the material carrying AA genotype.

This invention discloses the application of the GmABCB19ab gene in regulating soybean plant architecture. Using CRISPR-Cas9 gene editing technology, this invention created GmABCB19ab gene-edited mutant soybean lines. It was found that under both white light and shading conditions, compared to the wild-type Williams 82 (W82), the plant height of the GmABCB19ab gene-edited mutant soybeans was significantly reduced; the petiole angle of the GmABCB19ab gene-edited mutant soybeans was also significantly smaller. Therefore, loss-of-function editing of the GmABCB19ab gene in soybeans is one of the effective ways to alter plant architecture, and it has important production and theoretical significance for the breeding of superior soybean varieties and the development of germplasm resources.

Soybean plants, plant parts and plant cells capable of producing seeds comprising oil having relatively higher oleic acid levels and lower linoleic and linolenic acid levels than corresponding seeds lacking the targeted mutation.SOLUTION: Disclosed are TALEN compositions and methods of use, including using a multiplexing composition to make targeted mutations in several genes at once, such as the FAD3A / B / C genes, compositions for making targeted mutations in a single gene, such as a gene encoding a FAD2 protein, and combinations thereof. The compositions and methods can provide genetically edited plants, plant parts, and plant cells with improved characteristics as compared to corresponding unmodified plants, plant parts, or plant cells.SELECTED DRAWING: FIG. 1A

The invention discloses a chimeric immune receptor and application of the chimeric immune receptor to improvement of phytophthora resistance of plants, and belongs to the technical field of plant disease control. The chimeric immune receptor disclosed by the invention can specifically recognize the phytophthora sojae effector Avh85 or a homolog thereof, and can be activated only when a specific pathogenic bacterium effector exists, so that accurate recognition of target pathogenic bacteria is realized, and unnecessary energy consumption of a plant immune system is avoided. Once the chimeric immune receptor disclosed by the invention is activated, multiple and potent immunoreactions including significant up-regulation of defensive genes, active oxygen outbreak and MAPK phosphorylation can be initiated, a firm defensive barrier is formed together, and expansion of pathogenic bacteria can be rapidly and efficiently suppressed.

The present application relates to a nucleic acid sequence for detecting a soybean plant DBN8205 and a detection method thereof, the nucleic acid sequence comprising SEQ ID NO:1 or its complementary sequence, and / or SEQ ID NO:2 or its complementary sequence. The soybean plant DBN8205 of the present application has good resistance to lepidoptera insects and good tolerance to glufosinate herbicide, and has no effect on yield. The detection method can accurately and quickly identify whether the DNA molecule of the transgenic soybean event DBN8205 is contained in the biological sample.