16 results about "Soyauxia" patented technology

The application discloses a soybean plant height related InDel molecular marker and application thereof, relates to the technical field of biological molecule detection.The nucleotide sequence of the InDel molecular marker is shown as SEQ ID NO.4 or SEQ ID NO.5.The InDel molecular marker developed by the application can realize efficient, accurate and early identification of soybean plant height.The InDel molecular marker has stable polymorphism, high amplification efficiency of the matching specific primer, clear bands, good repeatability and clear typing.It is verified that the length difference of the amplification product is significantly related to the plant height phenotype, high-stalk and dwarf individuals can be accurately distinguished, and the genotype detection result is highly consistent with the field phenotype.The application provides a stable and reliable molecular tool for genetic improvement of soybean plant height and rapid cultivation of high-yield and lodging-resistant materials.

This invention relates to a GmSPL3b gene SNP marker and its application, belonging to the fields of plant molecular biology and crop genetics and breeding technology. The SNP marker is used for molecular detection of soybean flowering and maturity stages. The SNP is a single nucleotide mutation at position 94 and / or position 116 of the first exon of the soybean GmSPL3b gene, forming a nucleotide sequence as shown in SEQ ID NO. 1 and / or SEQ ID NO. 2; the polymorphism of the single nucleotide mutation is A / G polymorphism. This invention enables precise genotyping at the seedling stage, efficiently breeding early-maturing soybean varieties adapted to high-latitude regions. It can directly serve as a precise target for gene editing, providing a clear basis for molecular design breeding. It allows for rapid screening of ideal genotypes in early generations, reducing field workload, shortening the breeding cycle, and accelerating the selection of new varieties.

Provided are a nucleic acid sequence for detecting a Glycine max plant DBN8205 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof. The Glycine max plant DBN8205 has good resistance to insects of Lepidoptera order and good tolerance to glufosinate herbicides, and therefore the yield is not affected. By utilizing the detection method, it is possible to accurately and quickly identify whether a biological sample contains a DNA molecule of a transgenic Glycine max event DBN8205.

This invention discloses the use of an insecticidal protein, specifically a method and its application for controlling lepidopteran pests. The method includes contacting the lepidopteran pests with the insecticidal protein, or introducing a nucleic acid molecule encoding the insecticidal protein into a plant, causing the lepidopteran pests to feed on the plant. The insecticidal protein contains the amino acid sequence shown in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:20. In this invention, the Cry1Da1 proteins BD1-002, BD1-018, BD1-019, BD1-021, BD1-022, and BD1-022S exhibit good insecticidal activity against the soybean pest, the silver-striped armyworm.

The present application relates to the field of crop molecular biology technology, and particularly relates to a SNP marker for soybean flowering period phenotype identification and application thereof. The present application provides a SNP marker for soybean flowering period phenotype identification, which is located at the 12087053th position on the chromosome 2 of soybean, and the deoxynucleotide is T or A. The present application detects the genotypes of the 12087053th position on the chromosome 02 of soybean in dozens of sample materials by using whole genome resequencing method, and the results show that the average flowering time of the material carrying TT genotype is significantly earlier than the flowering time of the material carrying AA genotype.

This invention discloses the application of the GmABCB19ab gene in regulating soybean plant architecture. Using CRISPR-Cas9 gene editing technology, this invention created GmABCB19ab gene-edited mutant soybean lines. It was found that under both white light and shading conditions, compared to the wild-type Williams 82 (W82), the plant height of the GmABCB19ab gene-edited mutant soybeans was significantly reduced; the petiole angle of the GmABCB19ab gene-edited mutant soybeans was also significantly smaller. Therefore, loss-of-function editing of the GmABCB19ab gene in soybeans is one of the effective ways to alter plant architecture, and it has important production and theoretical significance for the breeding of superior soybean varieties and the development of germplasm resources.

The present application relates to a nucleic acid sequence for detecting a soybean plant DBN8205 and a detection method thereof, the nucleic acid sequence comprising SEQ ID NO:1 or its complementary sequence, and / or SEQ ID NO:2 or its complementary sequence. The soybean plant DBN8205 of the present application has good resistance to lepidoptera insects and good tolerance to glufosinate herbicide, and has no effect on yield. The detection method can accurately and quickly identify whether the DNA molecule of the transgenic soybean event DBN8205 is contained in the biological sample.

This invention belongs to the field of genetic engineering technology, and specifically discloses the soybean BSP family. Glyma.20G130000.1 Application of a gene in soybean resistance to soybean Phytophthora root rot. This invention clones a gene from soybean. Glyma.20G130000.1 The expression was efficiently achieved in soybean plants through transient transformation, resulting in overexpressed soybean plants. Functional identification results showed that, compared with control plants, the overexpression... Glyma.20G130000.1 Soybean plants containing this gene exhibit significantly enhanced resistance to Phytophthora soybeanii. Furthermore, this invention provides the application of biomaterials related to this gene, and methods for screening or breeding disease-resistant soybean plants based on this gene. Therefore, Glyma.20G130000.1 Genes not only provide an effective tool for revealing the basic resistance mechanism of soybean to Phytophthora root rot, but also provide valuable genetic resources for soybean disease resistance breeding.

This invention relates to the field of daidzein synthesis, specifically to a daidzein, its preparation method, and its applications. This invention synthesizes a novel daidzein by co-expressing genes AtPAL2, GmCHS8, GmCHR5, GmCHI1B1, GmIFS1, GmHID, and AtMYB60 (genes for daidzein synthesis), downstream daidzein synthesis genes GmI2'H, GmIFR, GmVR, GmPTS1, GmD6aH, and GmG2DT, and the daidzein synthase gene GmGS3e / 7 in *Nicotiana benthamiana* mediated by *Agrobacterium*, thus paving the way for heterologous daidzein biosynthesis. This daidzein effectively inhibits fungal activity, showing good inhibitory effects against *Phytophthora sacchari* and *Phytophthora xanthoides*, and has broad application prospects in green pest control and food development.

Compositions and methods for altering flowering and / or maturity time of soybean plant are provided. Compositions include isolated and recombinant polynucleotides encoding polypeptides, expression cassettes, host cells, plants, plant parts stably incorporating these polynucleotides. Methods and kits are provided for producing these plants via transgenic means, breeding or genomic editing approaches and identify plants having altered flowering and / or maturity time.

The present invention relates to synthetic enhancer elements that can be used to increase the expression of a gene of interest in any plant tissue. The disclosed synthetic enhancer elements can be used in a gene expression cassette comprising nucleic acid further comprising a promoter and a sequence to be expressed, wherein the sequence to be expressed has a transcription start site, and wherein the synthetic enhancer element is operably linked to the promoter and the sequence to be expressed. The present invention also relates to a modified plant or part thereof comprising a synthetic enhancer element, wherein the synthetic enhancer element is operably linked to an endogenous promoter in the modified plant or part thereof and an endogenous gene in the modified plant or part thereof, and wherein the synthetic enhancer element increases expression of a transcript or protein encoded by the endogenous gene.

Methods of prevention, control and / or suppression of fungal pathogens in soybean plants, such as those pathogens that cause target spot, Septoria brown spot, frog eye leaf spot, white mold, and / or anthracnose, and compositions useful in such methods are provided. The methods generally involve applying a composition comprising zoxamide to a soybean seed, soil, a soybean plant or field of soybean plants that comprise, or are susceptible to, a causal agent of target spot, Septoria brown spot, frog eye leaf spot, white mold, and / or anthracnose.

This invention discloses the application of soybean transcription factor GmMYB521 in plant flowering regulation, belonging to the field of biotechnology. The soybean transcription factor protein GmMYB521 disclosed in this invention is as follows: A1) a protein whose amino acid sequence is sequence 2; A2) a protein with the same function achieved by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in sequence 2 of the sequence listing. Experiments have shown that GmMYB521 is associated with plant flowering: overexpression compared to the wild-type receptor... GmMYB521 Genetically modified plants exhibit an early-flowering phenotype. (Explanation) GmMYB521 The gene and the protein it encodes have a positive biological function in regulating the flowering time of soybeans, and can promote the transformation of soybean vegetative growth to reproductive growth, which has important theoretical and practical significance for breeding early-flowering varieties of plants.

The application belongs to the technical field of biotechnology, and particularly discloses a kind of GmGIS3a Gene in the application of regulating soybean height and flowering time. The sequence of the GmGIS3a Gene is shown as SEQ ID NO.1, GmGIS3a The amino acid sequence encoded by the gene is shown as SEQ ID NO.2. The application overexpresses soybean GmGIS3a Gene by using pTF101 vector, transforms soybean cultivar Wm82, and obtains stable overexpression plants. Research finds that the overexpression plants are dwarf compared with wild type plants, and the flowering time is early, which indicates that the GmGIS3a GmGIS3a Gene is involved in the biological process of regulating plant height and flowering time. It provides a new breeding approach for cultivating dwarf and early flowering plant varieties, and has a good application prospect in the field of plant breeding technology.

This invention relates to the field of biotechnology and discloses a method for targeted improvement of ideal soybean plant architecture based on BR synthase gene editing. The method includes: integrating multi-omics data to construct a BR synthase gene regulatory network prediction model to accurately identify key functional sites affecting traits such as plant height and internode length; designing a CRISPR-Cas12a vector to edit target sites; achieving high-throughput, non-destructive phenotypic monitoring of T0 generation plants through a controlled-environment growth chamber combined with multispectral and lidar technologies; associating genotype and phenotypic data to screen for ideal plant architecture individuals, and optimizing the prediction model through data feedback to form a closed-loop iterative system. This invention shortens the breeding cycle, improves the predictability of editing, and efficiently obtains new soybean germplasm with compact growth, lodging resistance, and a high harvest index.

The application belongs to the technical field of automatic detection, and particularly relates to a soybean hybrid plant automatic detection method and system based on botanical morphological characteristics, which comprises data acquisition, extraction of pixel-level contours of soybean leaflets and flowers and phenotype categories; construction of leaf vein direction vectors and calculation of geometric parameters based on morphological characteristics by using the pixel-level contours; completion of preliminary grouping of the leaflets; weighted calculation of comprehensive leaf types based on the classification confidence of each leaflet in the preliminary grouping, verification of whether there are abnormal leaflets with conflicting phenotype characteristics in the preliminary grouping by using the comprehensive leaf types, and correction of the abnormal leaflets; and judgment of the soybean hybrid plants by comparing the standard feature library of the target breeding variety with the soybean hybrid plants by using the dual-trait cross verification mechanism of the comprehensive leaf types and flower colors. The application overcomes the vulnerability of single morphological characteristics to environmental stress interference, and realizes high-precision screening of field soybean hybrid plants with high robustness and low misjudgment rate without increasing the cost of three-dimensional hardware.