99 results about "Soyauxia" patented technology

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

Provided are a gene regulating a leguminous plant plant type having tolerance to high density, and a use thereof. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, the amino acid sequence of a protein encoded thereby is as shown in SEQ ID NO: 2, and the genome sequence is as shown in SEQ ID NO: 3. Transgenic leguminous plants having the gene have a reduced branch number, a reduced plant height, a reduced node number, a shortened internode length, and / or a shortened petiole length compared to control or wild-type plants.

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmPP2-10 in promoting growth of plants in a low-phosphorus stress environment. Researches show that the expression of the GmPP2-10 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the over-expression of the GmPP2-10 can increase the biomass of a transgenic plant and promote the growth of the plant under the low phosphorus condition; meanwhile, overexpression of GmPP2-10 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GmPP2-10 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved by transferring the GmPP2-10 into the plant through a transgenic technology.

The invention discloses application of a CSA1 gene in regulating and controlling branching angles and petiole angles of leguminous plants and creating close-planting-resistant high-yield plant type leguminous plants. According to the invention, a CSA1 gene is cloned from soybean, and a CRISPR-Cas9 system is further utilized to edit the CSA1 gene, so that two different mutants are obtained. A result shows that the branching angle and the petiole angle of a mutant plant are remarkably reduced, and the yield of the mutant is remarkably higher than that of a wild type under a high-density planting condition, so that the CSA1 gene can regulate and control the branching angle and the petiole angle of the leguminous plant and create a close-planting-resistant leguminous plant, an important gene resource is provided for molecular breeding of the leguminous plant, and the application prospect is wide. And an effective means is provided for creating close-planting-resistant high-yield leguminous plants.

The invention relates to the technical field of agricultural biology, discloses a soybean shade-tolerant gene GmUGT73C2 as well as a molecular marker and application thereof, and is characterized in that the gene GmUGT73C2 which has a remarkable regulation effect on the plant height and shade tolerance of soybeans is obtained through screening and verification; the plant height of the wild soybean is remarkably reduced and the shade tolerance of the soybean is improved by overexpression of the gene in the soybean plant. A novel gene and a novel method are provided for construction of dwarf and shade-tolerant soybean plants. Meanwhile, an SNP locus corresponding to the gene is found, a corresponding molecular marker is developed, and an efficient screening tool is provided for construction of dwarf and shade-tolerant plants.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance and methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenes and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

Compositions and methods for altering the flowering and / or maturing time of soybean plants are provided. Compositions include isolated and recombinant polynucleotides encoding polypeptides, expression cassettes, host cells, plants, plant parts that stabilize incorporation of these polynucleotides. Methods and kits for producing these plants via transgenic means, breeding, or genome editing methods and identifying plants with altered flowering and / or maturation times are provided.

The invention belongs to the technical field of molecular breeding, and particularly relates to application of an E1-SOC1 gene model in cultivation of wide-adaptability soybean varieties. Research finds that three genes (E1 and homologous genes E1La and E1Lb) of an E1 family and two genes (SOC1a and SOC1b) of an SOC1 family are knocked out from soybeans at the same time by utilizing a gene editing technology, and the obtained homozygous five mutants show wide adaptability when planted under field conditions of different latitude regions; and the method has certain wide adaptability in the aspects of plant height, section number, flowering period, mature period and yield. It is indicated that the E1-SOC1 gene module (E1, E1La, E1Lb, SOC1a and SOC1b genes) is expected to be applied to molecular design breeding for breeding wide-adaptability soybean models, and has important application prospects in soybean molecular breeding research.

The present invention relates to a method for controlling pests of a modified plant, in particular a soybean plant, comprising the step of contacting the plant, a part thereof, a propagation material thereof, a pest, a food supply, a habitat or a breeding ground thereof with one or more compounds of formula I, wherein the variables are defined as described in the specification.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR39 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The invention relates to the technical field of microorganisms, and provides a rhizobium mutant and application thereof, the rhizobium mutant is HH103 delta nuo, and is a mutant obtained by taking sinorhizobium fredii (sinorhizobium fredii) HH103 as a starting strain and performing gene knockout on a nuo control unit in an NADH oxidoreductase subunit I of the sinorhizobium fredii HH103. When the HH103 delta nuo is inoculated in the soybean seedling stage, the plant shows that the leaves are darker green, the plant biomass is up-regulated, the nitrogenase activity of a single plant is obviously higher than that of a wild type strain, and the plant height is integrally increased, so that the HH103 delta nuo improves the symbiotic effect of the rhizobium and the soybean.

Methods and compositions are provided for modifying the flowering time and / or maturity time of soybean plants to enable them to be cultivated in a variety of geographical locations having different day lengths. Modified soybean plants are disclosed comprising non-natural mutant alleles at the E1 and / or E1Lb locus. The modified plants have a shorter flowering and / or maturity time than control plants.

The invention relates to the technical field of molecular genetic breeding, and discloses a soybean plant type gene cloning and multiple PCR (Polymerase Chain Reaction) rapid identification method and application, comprising a PCR detection method of a stem apical clustered pod material and a stem apical normal pod material, molecular markers for gene positioning, cloning and plant type identification, and a PCR specific reaction system and conditions. The detection method can be applied to the fields of variety resource identification, transgenic breeding, gene editing breeding, molecular marker selective breeding, gene function research and the like. The method has the advantages of economy, rapidness, simplicity in operation and the like.

The invention relates to a cosmetic and / or dermatological composition comprising a Pterocarpus soyauxii wood extract, as a depigmenting or skin-whitening agent. The wood extract is present in the composition at a content ranging from 0.05 to 10% by weight relative to the total weight of the composition, and comprises a high proportion of ellagitannins and / or gallotannins. The extract is in particular a hydroglycerinated extract obtained by extraction in a mixture of water and glycerin, preferably assisted by ultrasound. The invention also relates to a composition containing the wood extract, to a non-therapeutic method for depigmenting or whitening the skin, and to a non-therapeutic use.

The invention provides application of a soybean GmWRKY33c gene in shade-tolerant soybean breeding and plant type regulation and control, and belongs to the technical field of functional gene breeding. The nucleotide sequence of the soybean GmWRKY33c gene provided by the invention is as shown in SEQ ID NO: 1. And transferring into soybean under the mediation of agrobacterium tumefaciens to obtain a transgenic plant with stable overexpression of the GmWRKY33c gene. A detection result shows that overexpression of the GmWRKY33c gene can reduce the plant height of the soybean, especially can significantly inhibit plant height increase caused by shading stress, and enables the soybean to maintain a proper height in a shading environment. The invention provides a new gene resource for ideal soybean plant type soybean breeding and shade-tolerant soybean breeding.

The present invention provides a genetically modified leguminous plant and a plant cell or plant part thereof wherein the activity and / or level of at least two endogenous polypeptides selected from the group consisting of polypeptides comprising the amino acid sequence of SEQ ID NO: 3, 6, 9 or 12 in the genetically modified leguminous plant is reduced or eliminated as compared to an ungenetically modified control plant. The invention also provides methods of making the genetically modified leguminous plants and plant cells or plant parts thereof.

The invention relates to the technical field of agricultural biology, in particular to SNP related to soybean plant height and a KASP primer and application thereof, S1855999953 remarkably related to soybean plant height is located at the 55999953bp position of the No.18 chromosome of a soybean genome Wm82. A4. V1, the site polymorphism is G or A, and both the GG genotype and the AA genotype can provide technical support for molecular marker-assisted breeding of soybean plant height characters. The KASP molecular marker is further developed, specific distinguishing and detection can be directly conducted on G or A basic groups of SNP mutation sites, the soybean plant height can be rapidly and accurately identified through the marker, the influence of the environment on soybean plant height character identification is reduced, the breeding period is shortened, and selection and improvement of soybean plant height characters are accelerated.

Methods and compositions are provided for modifying the flowering time and / or maturity time of soybean plants to enable them to be cultivated in a variety of geographical locations having different day lengths. Modified soybean plants are disclosed comprising non-natural mutant alleles at the E1, E2, E3, E4, E1La and / or E1Lb locus. The modified plants have a shorter flowering and / or maturity time than control plants.

Methods and compositions are provided for modifying the flowering time and / or maturity time of soybean plants to enable them to be cultivated in a variety of geographical locations having different day lengths. Modified soybean plants are disclosed comprising non-natural mutant alleles at the E1, E2, E3, E4, E1La and / or E1Lb locus. The modified plants have a shorter flowering and / or maturity time than control plants.

The application discloses a soybean plant height related InDel molecular marker and application thereof, relates to the technical field of biological molecule detection.The nucleotide sequence of the InDel molecular marker is shown as SEQ ID NO.4 or SEQ ID NO.5.The InDel molecular marker developed by the application can realize efficient, accurate and early identification of soybean plant height.The InDel molecular marker has stable polymorphism, high amplification efficiency of the matching specific primer, clear bands, good repeatability and clear typing.It is verified that the length difference of the amplification product is significantly related to the plant height phenotype, high-stalk and dwarf individuals can be accurately distinguished, and the genotype detection result is highly consistent with the field phenotype.The application provides a stable and reliable molecular tool for genetic improvement of soybean plant height and rapid cultivation of high-yield and lodging-resistant materials.

The present invention discloses an application of the PUB62 gene in regulating plant resistance to geminiviruses and a method for cultivating transgenic plants, wherein the geminiviruses are soybean yellow leaf curl virus, tomato yellow leaf curl virus, and Yunnan tomato leaf curl virus. In the present invention, the PUB62 gene of Nicotiana benthamiana is cloned and constructed into the NbPUB62-YFP vector carrying a yellow fluorescent protein. Through the agrobacterium-mediated transformation method, the NbPUB62-YFP vector is introduced into plant cells, and through cell and tissue culture techniques, NbPUB62 overexpressing transgenic plants are obtained. The NbPUB62 overexpressing transgenic plants can significantly inhibit the infection of soybean yellow leaf curl virus, and can also significantly inhibit the infection of tomato yellow leaf curl virus and Yunnan tomato leaf curl virus.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR24 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The invention belongs to the field of plant genes, and relates to application and a method of a GmTOC1a gene or protein in regulation and control of soybean flowering. The application of the soybean biological clock gene GmTOC1a in flowering control is found, two homologous copies GmTOC1a1 (Glyma. 05G025000) and GmTOC1a2 (Glyma. 17G102200) of the GmTOC1a gene are directionally knocked out through a CRISPR / Cas9 gene editing technology, a soybean mutant with the GmTOC1a gene mutated is obtained, research finds that the mutant shortens the growth period of soybeans and advances the flowering time, and it is indicated that the GmTOC1a inhibits soybean flowering. Therefore, the GmTOC1a gene can be applied to change or expand the cultivation regionalism of soybeans, so that the soybeans can be sown at different latitudes, the planting region of the soybeans is expanded, and a theoretical basis and genetic resources are provided for breeding good varieties of the soybeans.

The disclosure relates to novel soybean plants, plant cells, plant parts, and nucleotide sequences in soybean plants comprising (i) a mutated endogenous AIP10a gene promoter and / or AIP10a gene 5' untranslated region (5' UTR), and / or (ii) a mutated endogenous AIP10b gene promoter and / or AIP10b gene 5' UTR, along with methods of using and making the same.

This invention relates to a GmSPL3b gene SNP marker and its application, belonging to the fields of plant molecular biology and crop genetics and breeding technology. The SNP marker is used for molecular detection of soybean flowering and maturity stages. The SNP is a single nucleotide mutation at position 94 and / or position 116 of the first exon of the soybean GmSPL3b gene, forming a nucleotide sequence as shown in SEQ ID NO. 1 and / or SEQ ID NO. 2; the polymorphism of the single nucleotide mutation is A / G polymorphism. This invention enables precise genotyping at the seedling stage, efficiently breeding early-maturing soybean varieties adapted to high-latitude regions. It can directly serve as a precise target for gene editing, providing a clear basis for molecular design breeding. It allows for rapid screening of ideal genotypes in early generations, reducing field workload, shortening the breeding cycle, and accelerating the selection of new varieties.

The invention relates to an application of 5HIAA (5-Hydroxyindole-3-acetic acid) in improvement of saline-alkaline resistance of plants. The chemical formula of the 5HIAA (5-Hydroxyindole-3-acetic acid) is C9H9NO3. The invention further relates to a preparation method of the 5HIAA. The compound is a derivative of indoleacetic acid (IAA) and has an additional hydroxyl (-OH) group located at the fifth position of an indole ring. Experimental results show that when 5HIAA is applied to root systems of arabidopsis thaliana, trifoliate orange and soybean, the saline-alkaline resistance of the plants can be improved, the main performance is that the lateral roots of the plants are increased, and the growth of the plants under saline-alkaline stress is promoted. The method has an important application value.

Provided are a nucleic acid sequence for detecting a Glycine max plant DBN8205 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof. The Glycine max plant DBN8205 has good resistance to insects of Lepidoptera order and good tolerance to glufosinate herbicides, and therefore the yield is not affected. By utilizing the detection method, it is possible to accurately and quickly identify whether a biological sample contains a DNA molecule of a transgenic Glycine max event DBN8205.

The invention relates to the technical field of plant biological breeding. The invention provides a flower specific promoter Ppel5 and an application thereof. The nucleotide sequence of the promoter Ppel5 is shown as SEQ ID NO. 1. The promoter can realize specific activation of a target gene in the soybean flower through a spatio-temporal expression regulation mechanism. The promoter element has dual application values in crop reproductive development basic research and precise molecular breeding technology development.