Construction and application of anti-sclerotinia sclerotiorum gene GmPR5 and GmPR5 transgenic plant

A sclerotinia and transgenic technology, applied in the field of genetic breeding, can solve problems such as unclear function of PR5
CN110862995AActive Publication Date: 2020-03-06NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2020-03-06

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Abstract

The invention discloses construction and application of an anti-sclerotinia sclerotiorum gene GmPR5 and a GmPR5 transgenic plant, and belongs to the technical field of genetic breeding. In order to research the sclerotinia sclerotiorum resisting mechanism of soybeans and accelerate the sclerotinia sclerotiorum resisting breeding process of the soybeans, the invention provides the anti-sclerotiniasclerotiorum gene GmPR5, wherein the nucleotide sequence of the anti-sclerotinia sclerotiorum gene GmPR5 is shown as SEQ ID No. 1; and a recombinant vector and a recombinant bacterium containing the gene. According to the invention, a transgenic soybean plant is obtained by overexpression of the disease-resistant gene GmPR5. Through post-phenotypic identification, the GmPR5 is determined to participate in the sclerotinia sclerotiorum resisting reaction, a new thought is provided for obtaining a soybean variety which is completely resistant to sclerotinia sclerotiorum, a basis is provided for further researching the function and the disease resisting mechanism of the gene, and effective molecular markers and gene resources are provided for molecular design and breeding of sclerotinia sclerotiorum resisting of soybeans.
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Description

technical field

[0001] The invention belongs to the technical field of genetic breeding, and in particular relates to the construction and application of a soybean sclerotinia-resistant gene GmPR5 and a GmPR5 transgenic plant. Background technique

[0002] Soybean (Glycine max) is an important food crop in my country and the world, and it is also one of the important sources of edible vegetable protein and edible oil. Soybean sclerotinia is a high-incidence disease with serious damage, and it is one of the three major diseases that cause the most serious loss of soybean yield in the world. Soybean sclerotinia is a fungal disease caused by Sclerotinias clerotiorum (Lib.) de Bary, which directly affects the quality and yield of soybean. When the disease is severe, the incidence rate is as high as 50% to 90%, or even extinction. Due to the limitations of traditional breeding methods, it is particularly important to use molecular methods to screen and identify effective disease...

Claims

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