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Soybean photoreceptor GmPLP1 and coding gene and application thereof

A technology of encoding gene and transgenic plant, applied in soybean photoreceptor GmPLP1 and its encoding gene and application field, can solve the problems of less research on soybean photoreceptor gene cloning and functional analysis, etc.

Inactive Publication Date: 2012-05-16
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the genes encoding Cryptochrome and Phytochrome have been cloned in soybean in recent years, there are still few studies on the cloning and functional analysis of soybean photoreceptor genes, and there are few reports in this regard
Especially the mechanism of regulating flowering after soybean receives light signal, that is, the conduction of photoreceptors in cells after receiving light signal; the influence on plant endogenous growth hormone; the regulation process of physiological activities, etc. in-depth study

Method used

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  • Soybean photoreceptor GmPLP1 and coding gene and application thereof
  • Soybean photoreceptor GmPLP1 and coding gene and application thereof
  • Soybean photoreceptor GmPLP1 and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Cloning of embodiment 1, GmPLP1

[0077] 1. Cloning of GmPLP1

[0078] 1) Extraction of plant total RNA

[0079] Soybean (Glycine max (L.) Merrill.) late-maturing variety Dongnong L13 (Lin Zhao and Wenbin Li*(2008) A RAV-like transcription factor control photosynthesis and senescence in soybean. Planta. 227: 1389-1399, available to the public from Northeast Obtained from Agricultural University.), with infinite pod habit, cultivated in 25℃ light incubator, 250μmol m -2 sec -1 White light, grow under long-day (LD) (16h / 8h light / dark) conditions, after the first three compound leaves unfold (10 days after emergence), start short-day (SD) photoperiod treatment, (8h / 16h light / dark) cultured on the 15th day after the treatment, when the dark / light alternated, samples were taken, and the third three-fold compound leaf was cut off, quick-frozen in liquid nitrogen, and stored at -80°C for total RNA extraction. Soybean total RNA was extracted according to the method of Triz...

Embodiment 2

[0176] Example 2, Obtaining and Phenotypic Research of Transgenic Tobacco Plants Overexpressing GmPLP1 Gene

[0177] 1. Acquisition of transgenic GmPLP1 tobacco

[0178] 1. Construction of Agrobacterium binary expression vector

[0179] The intermediate vector pGEMT-GmPLP1 obtained in Example 1 was double-digested with BamHI and Sac I, and the obtained small fragment was similarly digested with pBI121 (purchased from Beijing Baierdi Biotechnology Co., Ltd., product number: MP-091 ) the connection of the large pieces of vectors obtained, the obtained connection products were transformed into Escherichia coli DH5α, and the obtained transformants were identified by bacterial liquid PCR. The sub-extracted plasmid was identified by double enzyme digestion with BamHI and Sac I, and a plasmid with a size of 1768bp was obtained, and then sent for sequencing. The result was that the plasmid was inserted into pBI121 from the 3rd to 1706th nucleotides of the sequence 1 in the sequence t...

Embodiment 3

[0267] Example 3, Obtaining and Phenotypic Research of Transgenic Soybean Plants Overexpressing GmPLP1 Gene

[0268] 1. Obtaining GmPLP1 overexpression soybean

[0269] 1. Hypocotyl transformation method

[0270] Soybean (Glycine max (L.) Merrill.) (variety Heinong 44, purchased from Beidahuang Seed Industry Group Co., Ltd., hereinafter referred to as wild-type soybean.) was sterilized by chlorine gas for 16 hours, and then planted in germination medium (MS+ 3% sucrose+0.8% agar, pH5.8), after seven days, cut the hypocotyl of 1cm length as explant, soak 30min in the LBA4404 / pBI121-GmPLP1 bacterium liquid that obtains by embodiment 2; Use sterile filter paper Blot dry the bacterial solution on the surface of the plant material, place the hypocotyls flat on the co-cultivation medium (1 / 2 MS large amount of salt + MS iron salt + 1.67 mg / L 6-benzylaminopterin + 40 μM acetyl clove) covered with a layer of filter paper Ketone + 1mg / L sodium thiosulfate + 1mg / L cysteine ​​+ 1.0mg / L...

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Abstract

The invention discloses a soybean photoreceptor GmPLP1 and a coding gene and application thereof. The protein provided by the invention is named as GmPLP1 and is a protein as described in the following (1) or (2): (1) protein with an amino acid sequence as shown in the sequence 2 in a sequence table; and (2) protein which is derived from (1) by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of the sequence 2, and is associated with plant traits. The plant traits comprise at least one of the following (1)-(10): (1) branch number; (2) time of flowering; (3) plant height; (4) blade area; (5) distance between nodes; (6) root length; (7) photosynthetic rate; (8) stress tolerance; (9) stem diameter; and (10) node number. The photoreceptor gene GmPLP1 disclosed by the invention can be used for changing the plant morphology, increasing the plant branches and changing the time of flowering and ripening of the plant, and has great importance for plant breeding and research on the function and action mechanism of the photoreceptor gene.

Description

technical field [0001] The invention relates to a soybean photoreceptor GmPLP1 and its coding gene and application. Background technique [0002] Light is the most extensive, most obvious and most important environmental factor affecting plant growth and development. The regulation of light on plants is manifested in: photosynthesis efficiency, respiration intensity, differentiation of apical meristem to floral primordia (photoperiodic response), tropism and so on. The photoperiod response directly determines the yield, quality and adaptability of crops. So far, only the molecular mechanism of the light response of the model plant Arabidopsis thaliana has been studied extensively and in detail. [0003] In the light response, plants perceive external light signals through photoreceptors and transmit the light signals to the circadian rhythm clock system, thereby activating or inhibiting the expression of photoperiod flowering time genes to control the flowering time of pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 李文滨罗秋兰赵琳韩英鹏
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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