Bacillus tequilensis and application thereof

A technology of Bacillus tequila and Bacillus, applied in the application, bacteria, fungicides and other directions, can solve the problems of low colonization and proliferation ability, narrow antibacterial spectrum, low control effect, etc. Simple preparation method and low cost effect

Active Publication Date: 2020-05-19
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] At present, the existing biocontrol bacteria still have the problems of narrow antibacterial spectrum, low control effect, and low colonization and multiplication ability. Screening effective biocontrol bacteria is of great significance for the control of a wide variety of plant pathogens that are constantly evolving

Method used

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  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The acquisition and identification of embodiment 1 Tequila bacillus 2-2a

[0072] 1. Screening and isolation of Bacillus tequila 2_2a strain

[0073] (1) Sample collection: collect fresh leaves of soybean plants in the base of Cangzhou Academy of Agricultural Sciences, Hebei Province, rinse the surface with dust with sterile water, and then carry out surface disinfection of plant leaves (soak with 75% alcohol for 1 min and 8% Soak in NaClO for 4min to carry out surface disinfection treatment), wash with aseptic water 4 times;

[0074] (2) Separation and screening: cut the leaves into 1cm×1cm pieces, add water and grind them to a paste, let them stand for 10 minutes, spread them on LB plates, and culture them at 30°C for 48 hours;

[0075] (3) Purification: After the culture grows out, use the plate streaking separation method to purify, pick the colonies that grow at the highest concentration and streak on the enrichment medium until the pure culture is isolated.

[0...

Embodiment 2

[0096] The preparation of embodiment 2 bacillus tequila 2-2a bacterium liquid and bacterial agent

[0097] (1) Strain activation: Pick the Bacillus tequila 2-2a bacterial strain to the strain preservation medium, after continuous streaking at 30°C and picking a single colony for two times, pick a single colony in the strain activation medium, and 30°C, 180r / min shaker shaking culture for 24h;

[0098] (2) liquid seed preparation: in the fermenter that the seed culture medium of high temperature sterilization is housed, according to the 2-2a activation bacterial liquid that the inoculum size of 10% (V / V) obtains inoculation step (1), at 30 ℃, pass Air culture for 18 hours to obtain liquid seeds;

[0099] (3) Liquid state fermentation: In the seed culture medium equipped with high-temperature sterilization, inoculate liquid seeds according to the inoculum size of 10% by volume fraction, at 30 ℃, cultivate 24h (logarithmic growth phase) under 180r / min, obtain viable bacteria So...

Embodiment 3

[0101] The bacteriostasis of embodiment 3 Tequila bacillus 2-2a

[0102] The present embodiment analyzes Tequila bacillus 2-2a to soybean anthracnose bacterium (Colletotrichum chlorophyti), mouth protruding umbilicus (Exserohilum rostratum), Alternaria alternata (Alternaria alternata), Phoma herbarum (Phoma herbarum), gray spot fungus (Cercospora sojina), Fusarium oxysporum (Fusarium oxysporum) and Fusarium equiseti (Fusarium equiseti), the specific methods are as follows:

[0103] (1) Soybean anthracnose fungus (Colletotrichum chlorophyti), mouth protruding umbilical cordworm (Exserohilum rostratum), Alternaria alternata (Alternaria alternata), Phomaherbarum (Phomaherbarum), gray spot fungus (Cercospora sojina), Fusarium oxysporum Fungus (Fusarium oxysporum) and Fusarium equiseti (Fusarium equiseti) pathogens were inoculated on PDA plates respectively, and after being cultured at 25°C to cover the plates, bacteria cakes were made with a hole punch with a diameter of 5 mm, and...

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Abstract

The invention relates to the field of biotechnology, and particularly relates to bacillus tequilensis and application thereof. The bacillus tequilensis 2_2a provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No. 19446. The bacillus tequilensis 2_2a is capable of being quickly and stably colonized in soybean plants, high in propagation speed and strong in multiplication capacity, has a high-efficiency and broad-spectrum plant pathogen resistance, and has a combined treatment effect on plant diseases of overground parts such as anthracnose, leaf spot disease, black spot disease, gray spot disease, etc. and underground soil-borne diseases such as root rot disease, wilt disease, stem rot disease, blight disease, etc., with a high prevention effect and a good application prospect in prevention and control of plant diseases, thus laying a foundation for developing high-efficiency and broad-spectrumbiocontrol agents.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of bacillus tequila and its application. Background technique [0002] Soybean is a herbaceous plant with high nutritional value and strong adaptability. It is the most important crop in the world and one of the top five crops in the world. It plays a very important role in agricultural production and social development. Soybean disease is one of the main factors affecting soybean planting and yield. In recent years, climatic conditions and soil conditions are very favorable to the occurrence and spread of soybean diseases, and the existence of heavy cropping during soybean planting will increase the incidence of soybean diseases. Effective control methods of soybean diseases are of great significance for improving soybean yield and agricultural production efficiency. [0003] Among plant diseases, 70% to 80% are caused by the infection of pathogenic fungi. Plant fungal di...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/22A01P3/00C12R1/07
CPCA01N63/00C12P1/04C12N1/205C12R2001/07
Inventor 毕秋艳党志红韩秀英朱伟旗王文桥赵建江吴杰路粉高占林马建英
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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