Soybean leaf wrinkling disease field identification method
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An identification method, a technology of wrinkled leaves, which is applied in the field of field identification of wrinkled soybeans, can solve problems such as unclear wrinkled leaves, and achieve accurate and intuitive evaluation results
Active Publication Date: 2020-05-08
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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At present, there is no report on the method of field identification of this type of leaf wrinkling disease. What causes this type of leaf wrinkling disease is still unclear. Breeding and promotin
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Embodiment 1
[0037] Collect 10 spring soybean materials and 12 summer soybean materials with undetermined soybean leaf wrinkling symptoms, and observe the stability of each variety of soybean material wrinkled leaf disease in the field in 2018 and 2019 respectively. The method steps are as follows:
[0038] (3) Delineate the tested soybean plant area in the V6, R2 and R5 phases of soybeans, and count the number of soybean plants with different leaf wrinkling disease levels in the tested area;
[0039] Among them, there are 5 disease-level criteria for the classification of leaf fold disease, referring to figure 1 :
[0040] Level 0: normal, the canopy bean leaves have no wrinkles during the observation period, and the leaves are flat;
[0041] Level 1: Relatively normal, the canopy leaves are slightly wrinkled during the observation period, but the "hooked" shape does not appear, and the leaves can be flattened;
[0042] Level 2: Sensitive. During the observation period, the canopy leave...
Embodiment 2
[0055] A field identification method for soybean wrinkled leaf disease, comprising the steps of:
[0056] (1) Select Guixiadou 119, a variety with a high degree of wrinkled leaves and stable wrinkled leaves, as the control for the identification experiment of summer soybean wrinkled leaf disease resistance;
[0057] (2) During field identification, a row of control species is planted on both sides of the identified material at the same time, and a hole of control species is planted at the head of each row. At least 3 identification plots of each identification material are repeated; the specific planting mode refers to figure 2 .
[0058] (3) Delineate the tested soybean plant areas in the V6, R2 and R5 phases of soybeans, including the identified materials and controls, and count the number of soybean plants with different leaf wrinkles in the tested areas;
[0059] Among them, there are 5 criteria for classification of leaf fold disease:
[0060]Level 0: normal, the canop...
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Abstract
The invention relates to the technical field of plant disease identification, in particular to a soybean leaf wrinkling disease field identification method which comprises the following steps: (1) selecting a control material; (2) field planting test; (3) counting the number of soybean plants with different leaf wrinkling symptom levels; (4) calculating the disease index of the soybean leaf wrinkling disease; and (5) determining the leaf wrinkle resistance grade of the identified material. According to the method, the field occurrence situation of the soybean leaf wrinkling is investigated andstudied, the control variety with high leaf wrinkling degree and stable performance is tracked, and the control variety is used as a control group to divide the leaf wrinkling symptom level. According to the method, more than three experimental groups are arranged, the morbidity conditions of soybeans in the V6 stage, the R2 stage and the R5 stage are counted respectively, the disease index is calculated, and the leaf wrinkling disease resistance grade of the soybeans can be evaluated objectively by averaging the disease indexes. The evaluation result is accurate and visual. The identification method is used for identifying the leaf wrinkle resistance of different soybean germplasms, so that resistant varieties can be effectively screened out, and further research on the soybean wrinklingdisease is facilitated.
Description
technical field [0001] The invention belongs to the technical field of plant disease identification, in particular to a field identification method for soybean leaf wrinkling disease. Background technique [0002] In recent years, a kind of leaf wrinkling disease has been found on soybeans in Guangxi, Guangdong, Guizhou and other places. The leaves of plants with severe symptoms are severely shrunk, the leaf tips are bent downward into a "hook hand" shape, and the leaf veins are folded into an "S" shape. , with "bubble-like" bumps along the shrunken veins on the front of the leaf. When the symptoms are severe, the effective leaf area of leaves will be severely reduced, affecting soybean photosynthesis and reducing soybean yield by 10-20%. The study found that this type of leaf wrinkling in soybean can be stably inherited in a specific environmental area, and the materials with severe wrinkled leaves can cause large-scale leaf shrinkage when used in production. At present...
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IPC IPC(8): G01B11/00G01B11/24A01G22/40A01H1/04
CPCA01H1/04A01G22/40G01B11/00G01B11/24
Inventor 陈文杰郭小红汤复跃韦清源陈渊梁江
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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