Soybean leaf wrinkling disease field identification method
An identification method, a technology of wrinkled leaves, which is applied in the field of field identification of wrinkled soybeans, can solve problems such as unclear wrinkled leaves, and achieve accurate and intuitive evaluation results
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Embodiment 1
[0037] Collect 10 spring soybean materials and 12 summer soybean materials with undetermined soybean leaf wrinkling symptoms, and observe the stability of each variety of soybean material wrinkled leaf disease in the field in 2018 and 2019 respectively. The method steps are as follows:
[0038] (3) Delineate the tested soybean plant area in the V6, R2 and R5 phases of soybeans, and count the number of soybean plants with different leaf wrinkling disease levels in the tested area;
[0039] Among them, there are 5 disease-level criteria for the classification of leaf fold disease, referring to figure 1 :
[0040] Level 0: normal, the canopy bean leaves have no wrinkles during the observation period, and the leaves are flat;
[0041] Level 1: Relatively normal, the canopy leaves are slightly wrinkled during the observation period, but the "hooked" shape does not appear, and the leaves can be flattened;
[0042] Level 2: Sensitive. During the observation period, the canopy leave...
Embodiment 2
[0055] A field identification method for soybean wrinkled leaf disease, comprising the steps of:
[0056] (1) Select Guixiadou 119, a variety with a high degree of wrinkled leaves and stable wrinkled leaves, as the control for the identification experiment of summer soybean wrinkled leaf disease resistance;
[0057] (2) During field identification, a row of control species is planted on both sides of the identified material at the same time, and a hole of control species is planted at the head of each row. At least 3 identification plots of each identification material are repeated; the specific planting mode refers to figure 2 .
[0058] (3) Delineate the tested soybean plant areas in the V6, R2 and R5 phases of soybeans, including the identified materials and controls, and count the number of soybean plants with different leaf wrinkles in the tested areas;
[0059] Among them, there are 5 criteria for classification of leaf fold disease:
[0060]Level 0: normal, the canop...
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