SNP site relevant to Canadian Duroc backfat thickness and applications of SNP site

A technology of backfat thickness and location, applied in the field of molecular biology, can solve the problems of time-consuming and laborious, accelerate the genetic progress of backfat thickness, and achieve the effect of promoting the breeding process

Inactive Publication Date: 2017-03-22
ZHANGZHOU AONONG MODERN AGRI DEV CO LTD +6
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conventional breeding, the live pigs are measured by B-ultrasound first, and then the backfat thickness can be selected, but this work is time-consuming and laborious. Therefore, developing a fast, simple and efficient molecular breeding method has great significance in production. Big Necessity, Will Accelerate the Genetic Progression of Backfat Thickness

Method used

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  • SNP site relevant to Canadian Duroc backfat thickness and applications of SNP site
  • SNP site relevant to Canadian Duroc backfat thickness and applications of SNP site
  • SNP site relevant to Canadian Duroc backfat thickness and applications of SNP site

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Embodiment Construction

[0024] In order to explore the genetic mechanism of the backfat thickness of the plus-line Duroc, and develop a fast and efficient frontal molecular breeding technology related to the selection and breeding of backfat thickness, the present invention uses 83 Duroc populations, through genome-wide association (GWAS) Using methods such as analysis, we identified the SNP sites affecting Canadian Duroc, and identified the CACNA1E gene as its candidate gene. On this basis, the present invention also constructs a fast and efficient molecular detection technology. Through the in-depth study of the phenotypes corresponding to different genotypes, the genotypes related to the backfat thickness were determined, and the genetic progress of the backfat thickness can be accelerated through the selection of the genotypes.

[0025] 1. Experimental animals

[0026] 83 Canadian Duroc experimental groups, 83 Duroc phenotypes are distributed as follows: figure 1 shown.

[0027] 2. Experimenta...

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Abstract

The invention discloses an SNP site relevant to the Canadian Duroc backfat thickness and applications of the SNP site. The SNP is located in the CACNA1E gene on the swine No.9 chromosome, the SNP site of the CACNA1E gene is ALGA0055091 (G / A), the individual with the genotype AA of the site has the backfat thinner than that of the individual with the genotype GG of the site, on the basis, ALGA0055091 can be taken as an SNP site, through the selection for the genotype, the selection for the Canadian Duroc backfat thickness can be accelerated, and meanwhile, the genetic progress for improving the meat factor of the Canadian Duroc is accelerated.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a CACNA1E gene affecting the traits of backfat thickness and lean meat percentage of Canadian Duroc and its application in genetic improvement of breeding pigs. Background technique [0002] Duroc is native to New Jersey and New York in the eastern part of the United States. The main parent is a hybrid of Duroc from New York State and Jersey Red from New Jersey. It was originally called Duroc Jersey and later collectively called Duroc. Hejia Duroc; Duroc produced in Taiwan, my country has been cultivated into its own style, so it is called Taiwan Duroc or Taiwan Duroc. [0003] Duroc pigs have the advantages of good adaptability, no stress sensitivity, easy feeding and management, fast weight gain, high feed remuneration (feed-to-meat ratio 2.8-3.2:1), good carcass quality, and high lean meat percentage. It is suitable for factory pig raising and farmer raising. [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 龙毅万文峰廖玲玲李亮吴有林周盛昌肖丽萍敖大国颜勇周亚丽刘自芹
Owner ZHANGZHOU AONONG MODERN AGRI DEV CO LTD
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