72results about How to "Delayed flowering" patented technology

The present invention provides methods for obtaining plants that exhibit useful traits by expression of a DNA methyltransferase fusion protein in progenitor plants. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that express a DNA methyltransferase fusion protein are also provided.

The invention relates to a method for regulating flowers and spikes of Feizixiao litchis, belonging to the field of application of litchi production technology. The best method for gaining high and stable yields is to reduce the number of flowers and delay the time of flowering as the Feizixiao litchi variety is easy to flower, the number of flowers is great, the flowering needs to consume a great deal of nutrition and influence the rate of bearing fruit, and the fruit bearing is influenced by weather; the method comprises the steps: when spikes of Feizixiao litchis grows by differentiation to be 10-15 cm long in January and February, a growth retardant uniconazol with concentricity of 50-100ppm is used for postponing the growth of the spikes and restraining the premature growth of the spikes of Feizixiao litchis; after the method is adopted to treat the spikes, the premature growth of the spikes can be restrained to lead the spikes to become short and strong and greatly reduce the number of flowers, thus omitting heavy and nervous work for flower thinning for the Feizixiao litchis; and the flowering time of the Feizixiao litchis is postponed at about spring equinox, thus achieving the purpose of one-time flowering and bearing fruit, greatly improving rate of bearing fruit, and being compact in spikes, uniform in size of fruits and consistent in mature period.

The invention discloses a pepper planting method. The method comprises the steps of a, seedbed selection; b, seed treatment including, by means of a seed soaking and germination method, putting seeds into gauze bags, binding the mouths of the gauze bags, soaking the bags into water of 50-55 DEG C, continuously mixing the water until the water decreasing the temperature to 30 DEG C within 10 minutes, continuously soaking the bags for 5-6 hours, and taking the seeds out and cleaning the seed for germination acceleration; c, seeding; d, seedling growth period; e, field planting including arranging a square field at a width of 1.1 meters and a height of 20-25 centimeters, thoroughly watering the field 10 days before the field planting, after the surface of the field becomes dried, harrowing the field into diffused round shapes through a rake, applying black mulching film on the field and burying and treading both sides and both ends of the film into the soil, and then performing the field planting; f, flowering and fruiting period including controlling the daytime temperature to be between 20 and 25 DEG C and the night temperature to be between 16 and 20 DEG C. Peppers planted through the pepper planting method are flourishing in flowering and fruiting and can prolong the maturing period to improve the fruiting rate and the yield rate.

The invention provides application of a gene OsCOL16 to control of a rice heading stage. The gene OsCOL16 is derived from a rice variety Nipponbare, a cDNA sequence of the gene OsCOL16 is as shown in SEQ ID NO:1, and the gene OsCOL16 codes B-box/CCT zinc finger protein with 448 amino acids and belongs to a CONSTANS-like transcription factor gene family. According to genetic transformation experiments, overexpression of the gene OsCOL16 inhibits rice heading under both short-day and long-day conditions; according to further researches, the gene OsCOL16 is in day-night rhythm expression and located in cell nucleuses and has transcription self-activation activity, and expression of Ehd1, Hd3a and RFT1 is inhibited by up-regulation of Ghd7 to finally delay heading delay.

The invention provides modified oleosins, including at least one artificially introduced cysteine, and methods and compositions for producing the modified oleosins. Also provided are polynucleotides encoding the modified oleosins, constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the modified oleosins, in vivo and in vitro, and methods for producing oil in host cells and plants. The invention also provides methods for increasing the rate of CO₂ assimilation in photosynthetic cells and plants, and involves reducing or preventing lipid recycling, and/or expressing modified oleosins with artificially introduced cysteine residues in the photosynthetic cells and plants. Also provided are methods for increasing oil production in plants, via expression of modified oleosins in the non-photosynthetic tissues/organs of plants. The method also optionally includes the step of extrating the oil from the non-photosynthetic tissues/organs of the plant, or processing the oil rich non-photosynthetic tissues/organs into animal or biofuel feedstocks.

The invention discloses an application of soybean E3 ubiquitin ligase gene GmPUB2 capable of regulating flowering of plants, namely, the application of the oybean E3 ubiquitin ligase gene GmPUB2 in regulating flowering of plants. A late-flowering plant is obtained after the GmPUB2 gene is transplanted into a target plant through a plant expression vector; an early-flowering plant is obtained after the GmPUB2 gene is transplanted into a target plant through a gene silencing carrier; after the target gene GmPUB2 is transplanted into arabidopsis thaliana, compared with the wild arabidopsis thaliana, the results show that the bolting time and the flowering time of the transgenic plant are obviously later than those of the wild plant, namely, the GmPUB2 gene has the function of delaying the flowering of the plants. With the established GmPUB2 gene silencing carrier to silence the transgenic arabidopsis thaliana with over-expressed target gene GmPUB2, the results show that the flowering time of the plant after silencing is obviously earlier than that of a control plant.

The invention belongs to the technical field of plant cytology, and particularly relates to a method for identifying haploid regenerated by brassica napus microspores simply, conveniently and quickly in batch in the early stage in a method of chloroplast counting. The method is characterized by comprising the following steps of: inoculating an embryoid cultured by in-vitro microspores onto a B5 culture medium; when the embryoid is grown into a complete plant, cutting the fifth even leaf sterilely; dyeing the lower epidermis tissue in the middle of the leaf by using iodine-potassium iodide; observing chloroplasts of guard cells of air vents by using an optical microscope; and recording the number of the chloroplasts of 10 air vents on each prepared leaf (namely the same leaf), wherein more than 7 air vents have 6 to 8 chloroplasts, and the plants of the chloroplasts are the haploid. By the method, the problem of long-time subculture of the haploid is solved, and in ten thousands of plants, the cost of reagents, lands and labors can be saved by 19,083 RMB. The method has a simple process, is easy to operate and low in cost and can be used for identifying the ploidy of a large number of plants, and one plant of seedling is identified only for 5 minutes, so the application efficiency of the double haploid (DH) technology is improved.

The invention discloses application of an OsBICs gene in regulating plant height and flowering time of a plant. The OsBICs gene comprises rice genes OsBIC1 and OsBIC2. Amino acid sequences of encodedproteins of the rice genes OsBIC1 and OsBIC2 are correspondingly as shown in SEQ ID NO. 2 and 4. The application of the OsBICs gene in regulating the plant height and the flowering time of the plant,disclosed by the invention, has the advantages that the rice genes OsBIC1 and OsBIC2 are obtained through the first time of cloning, functions of the rice genes OsBIC1 and OsBIC2 are researched, it isfound that effects of the OsBIC1 and the OsBIC2 in the rice photoperiodic reaction are obvious; characteristics of the OsBIC1 and the OsBIC2 are similar to those of arabidopsis thaliana BICs; and theOsBIC1 and the OsBIC2 can both delay flowering and blue light specificity to promote the elongation of a leaf sheath.

The invention provides a method for efficiently increasing production of cassia sophera seeds which are used as tea garden green manure. The method comprises the following steps: selecting ground for a cassia sophera nursery garden, sowing, transplanting, performing disease and insect pest and weed control, and removing terminal buds at a proper time to promote development of lateral branches and improve ripening rate of the seeds; and when harvesting the seeds, hammering the pods by bamboo poles to enable the seeds of tea fertilizer No.1 to be piped. The method is simple to operate, high in ripening rate, and cannot influence growth of tea trees in the tea garden.

The invention discloses an application of a corn gene ZmSCL14 in regulation and control of a flowering period of a plant. The nucleotide sequence of the corn gene ZmSCL14 is as shown in SEQ ID NO. 1.According to the invention, a recombinant overexpression vector is obtained by utilizing the existing plant genetic engineering technology, then brachypodium distachyon callus is transformed by utilizing an agrobacterium infection transformation method, and a homozygous brachypodium distachyon plant of the overexpression ZmSCL14 gene is obtained through tissue culture, identification and screening. Compared with a wild type plant growing under the same condition, the brachypodium distachyon over-expressing the ZmSCL14 gene shows a late flowering phenotype, delays blooming of the brachypodium distachyon, can slow down the growth speed of the brachypodium distachyon plant and participates in regulating and controlling expression of blooming-related genes, so that the ZmSCL14 gene can be applied to cultivation of late flowers or dwarf varieties, and high agricultural application values are realized.

The invention provides a method for producing hybrid seeds by use of pepper genic male sterile line and restorer line. The method comprises the following steps: 1, planting a pepper genic male sterile AB line and a restorer line in an area or a greenhouse where pepper plants are capable of overwintering cultivation respectively, taking sterile plants of the genic male AB line as female parent and taking the restorer line as male parent to perform hybridization so as to produce hybrid seeds; 2, pruning aerial parts of the sterile plants and the restorer line plants after the harvest of hybrid seeds in winter and then performing overwintering cultivation; 3, pruning and tidying dormant buds of the overwintering plants after the dormant buds sprout next year, taking the sterile plants as the female parent and taking the restorer line as the male parent to perform hybridization during florescence so as to produce hybrid seeds; and 4, repeating a step 2 and a step 3 three to four times. The method can continually harvest pepper hybrid seeds in a plurality of years after seeding once in a year, saves labor and time for hybrid seed production, is low in cost and long in heterosis-holding time, capable of guaranteeing the yield and quality of hybrid peppers and convenient for commercial application, and has important significance for popularizing the technology of utilizing pepper heterosis.

The invention provides an astragalus sinicus LHY gene and application thereof. The LHY gene of astragalus sinicus and a sequence of a protein encoded by the LHY gene are shown in SEQ ID NO: 1 and 2. The LHY gene is cloned from the astragalus sinicus for the first time, and the LHY gene is verified to have a function of regulating and controlling plant flowering, in particular, delaying plant flowering, in arabidopsis thaliana through an agrobacterium-mediated genetic transformation method. The LHY transgenic arabidopsis thaliana shows early bolting and early flowering, bolting days are 18.36 days later than wild type, and flowering days are 20.72 days later than wild type. This indicates that LHY gene of astragalus sinicus is closely related to flowering, and has a function of regulating flowering time. The gene is applied to plant property improvement, and has good application prospect. The LHY gene and the encoded protein thereof provide valuable resources for cultivating new plant varieties.

The invention discloses a peony flowering phase regulation and control method. The method comprises the steps that peony plants are selected, and diseased roots of the peony plants are removed; root wounds of the peony plants are disinfected, and the peony plants are dipped in rooting powder liquid to promote rooting of the peony plants; the peony plants are put into a pot, the pot is filled witha culture medium, and the peony plants are watered thoroughly after being cultivated; from the bud raising stage to the bud standing stage, the back faces of leaves of the peony plants are coated witha peony flowering phase advance regulator or a peony flowering phase delay regulator; and from the strong wind boll stage to the peach orchard stage, the back faces of the leaves of the peony plantsare coated with the peony flowering phase advance regulator or the peony flowering phase delay regulator. By applying the peony flowering phase advance regulator or the peony flowering phase delay regulator, peony plants can flower in advance or flower in a delayed mode, the total flowering phase of peony is prolonged as a whole, the ornamental time of peony is prolonged, operation is easy, the input cost is low, time and labor are saved, and peony flowering phase regulation and control in large-scale production can be met.

The invention belongs to the field of plant genetic engineering, and particularly relates to a method for specifically knocking out a soybean SOC1 gene through CRISPR/Cas9 and application of the method. The CRISPR/Cas9 knockout vector capable of simultaneously performing targeted editing on coding regions of homologous genes SOC1a and SOC1b in the soybean is obtained through construction, and the knockout vector is subjected to stable genetic transformation by utilizing an agrobacterium-mediated method to obtain a homozygous soc1a1b double-mutant soybean plant. Compared with a control soybean plant, the soc1a1b double mutant plant shows obvious phenotypes of delayed flowering period and mature period and increased node number, and the soybean yield and the regional adaptability of varieties are remarkably improved.

The invention discloses application of a flos chrysanthemi CmSVP gene. The application comprises the following steps of designing a specific primer of the CmSVP gene according to a CmSVP full-length sequence of obtained transcriptome data; performing PCR amplification; performing gel cutting, recycling and purification on a target strip after electrophoresis detection; connecting recovered DNA toa pEASY-Blunt vector, performing transformation to obtain an escherichia coli competence, and selecting 6 monoclonal sequencing to obtain pEASY-Blunt-CmSVP; performing gene comparison on sequences after sequencing through a Blast program of a NCBI network station, and predicting an ORF region of the flos chrysanthemi CmSVP gene and a presumed amino acid sequence by using an ORF finder; analyzing the physical and chemical properties of flos chrysanthemi CMSVP proteina by using an Expasy online analysis software; constructing system development evolutionary trees of different plant SVP proteinsby using an MEGA6.0 software; performing genetic transformation the CmSVP genes in flos chrysanthemi to culture plants to obtain a transgenic flos chrysanthemi variety obviously different from the wild type flos chrysanthemi, wherein a flowering time of the transgenic flos chrysanthemi is later than that of the wild type flos chrysanthemi, and elongation growth of a stem is superior to that of thewild type flos chrysanthemi.

The invention discloses a cut flower fresh-keeping tablet containing Trichoderma pseudokonii polysaccharide and a preparation method thereof. The tablet includes Trichoderma pseudokonii polysaccharide, salicylic acid, maleamide, fresh flower nutrient solution, citric acid , dextrin, soluble starch, sodium carboxymethyl starch, magnesium stearate, 10%-15% starch slurry, this cut flower preservation sheet is easy to use, easy to store, non-toxic and pollution-free, and has a good fresh-keeping effect for cut flowers, and can be applied to Preservation of various cut flowers.

The invention discloses a tung cake organic fertilizer special for perennial flowers and a preparation method thereof. The tung cake organic fertilizer is characterized in that tung cakes, tung oil, tung shells, tobacco stems, ground phosphate rock, people and livestock urine, chicken manure and biological humic acid serve as main raw materials, nutrients needed by the perennial flowers in the growth period are scientifically matched, the supply conditions and quantity of carbon, hydrogen, oxygen and nitrogen necessary for a developing molecular compound are stabilized, iron, manganese, molybdenum, copper and magnesium with the function of adjusting and changing colors of the flowers are selected to participate in synthesis of the developing molecular compound, and a traditional skill and multiple fermentation technologies are adopted for carrying out step-by-step curing and superposed decomposition on the materials; the finished product can promote normal growth of plants, the flowers develop attractively, soil fertility can be bettered, soil is sound, and plant diseases and pests are prevented and treated.