Method for specifically knocking out soybean SOC1 gene by CRISPR/Cas9 and application of method

A SOC1, specific technology, applied in the field of plant genetic engineering and bioengineering, can solve problems such as unclear specific functions, achieve the effect of increasing the number of main stem nodes, increasing the yield of a single plant, and increasing the yield
CN113265418AActive Publication Date: 2021-08-17GUANGZHOU UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU UNIVERSITY
Publication Date
2021-08-17

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Abstract

The invention belongs to the field of plant genetic engineering, and particularly relates to a method for specifically knocking out a soybean SOC1 gene through CRISPR / Cas9 and application of the method. The CRISPR / Cas9 knockout vector capable of simultaneously performing targeted editing on coding regions of homologous genes SOC1a and SOC1b in the soybean is obtained through construction, and the knockout vector is subjected to stable genetic transformation by utilizing an agrobacterium-mediated method to obtain a homozygous soc1a1b double-mutant soybean plant. Compared with a control soybean plant, the soc1a1b double mutant plant shows obvious phenotypes of delayed flowering period and mature period and increased node number, and the soybean yield and the regional adaptability of varieties are remarkably improved.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, relates to the field of plant genetic engineering, in particular to a method for specifically knocking out soybean SOC1 gene by CRISPR / Cas9 and its application. Background technique

[0002] Soybean [Glycine max(L.)Merr.] provides human beings with vegetable protein and vegetable oil, as well as industrial raw materials, and is closely related to human life. Cultivated soybeans were domesticated in the Huanghuaihai region of China about 5,000 years ago, and are known as the "king of beans". Soybean is a typical short-day dicotyledonous plant, which is extremely sensitive to photoperiod. The flowering time determines the regional adaptability, sowing time and harvesting time of soybean. SOC1 is an important flowering-inducing gene in Arabidopsis, which is ubiquitous in a variety of angiosperms. Although the functions and molecular regulatory pathways of SOC1-like genes regulating flowering and pla...

Claims

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