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Application of GmLCL3 gene in regulating photoperiod and flowering time of soybeans and increasing yield of soybeans

A technology of flowering time and photoperiod, applied in the genetic field, can solve problems such as unclear molecular mechanisms, achieve the effect of inhibiting plant flowering and have broad application prospects

Active Publication Date: 2021-03-30
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular mechanism of how the LHY / CCA1 gene participates in the regulation of soybean photoperiod flowering in soybean is not clear.

Method used

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  • Application of GmLCL3 gene in regulating photoperiod and flowering time of soybeans and increasing yield of soybeans
  • Application of GmLCL3 gene in regulating photoperiod and flowering time of soybeans and increasing yield of soybeans
  • Application of GmLCL3 gene in regulating photoperiod and flowering time of soybeans and increasing yield of soybeans

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Experimental program
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Effect test

Embodiment 1

[0037] 1. Construction of soybean lcl3 mutant

[0038] (1) Find the full-length sequence of LCL3 through the soybean genome online search software Phytozome (https: / / phytozome.jgi.doe.gov) (the amino acid sequence is shown in SEQ ID NO.1; the nucleotide sequence is shown in SEQ ID NO.2 shown), according to the exon sequence of the GmLCL3 gene in the soybean genome, a target primer (upstream primer F1: 5'-AAGAGAACG ATGGACAGAGG-3 '; Downstream primer R1: 5'-AAACCCTCTGTCCATCGTTCTCT-3') (for target location information and mutant plant editing methods, see figure 1 ), the oligonucleotide chain primers used were synthesized by Ruibo Sequencing Company.

[0039](2) The pYLgRNA vector used in the mutant creation experiment is transformed from the pUC18 vector with a size of about 3kb; the Cas9 vector pYLCRISPR / Cas9-DB is about 15.5kb in size and contains a Bar gene driven by a 35S promoter and a 35S promoter Driven Cas9 gene, terminator, ccdB E. coli lethal gene; pYLgRNA vector, pY...

Embodiment 2

[0048] 1. Phenotypic identification of LCL3 near-isogenic plants

[0049] (1) Screen the genetic variation of LCL3 from 720 resequenced varieties (sequenced by Guao Fund Company, and the sequenced varieties are collected from various regions in China and some parts of the world by the Molecular Genetics and Evolution Innovation Research Center of Guangzhou University) to obtain An effective natural variation, the variation is an amino acid substitution, resulting in a stop codon, which can cause the LCL3 protein to terminate prematurely (at position 478, the base A is mutated to T, and the amino acid K is mutated to a terminator), which is named: soybean Mutant Harosoy (LCL3).

[0050](2) By combining the soybean variety Harosoy (LCL3) with PI 628930 (references: Fang C, Chen L, et al. Rapid identification of consistent novel QTLs underlying long-juveniletrait in soybean by multiple genetic populations and genotyping-by-sequencing [J]. Mol Breeding, 2019, 39:1-11.) Crossing a...

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Abstract

The invention discloses an application of a GmLCL3 gene in regulating the photoperiod and flowering time of soybeans and increasing the yield of the soybeans. According to the invention, a GmLCL3 soybean mutant plant is obtained by using a CRISPR / CAS9 technology, and the GmLCL3 gene in soybeans is mutated, so that the growth period of the soybeans can be prolonged, the blooming of the plant is inhibited, and the yield of the soybeans is increased. Therefore, by constructing a GmLCL3 soybean mutant plant, the sensitivity of soybeans to the photoperiod can be changed, the flowering of the soybeans can be delayed, the growth period of the soybeans can be prolonged, and the yield of the soybeans can be increased. The discovery of the invention has very important academic value for further revealing the molecular mechanism of photoperiod regulation and control of soybean flowering, and provides theoretical basis and genetic resources for promoting the breeding of high-yield and high-qualityvarieties of soybeans.

Description

technical field [0001] The invention relates to the field of genes, in particular to the application of a GmLCL3 gene in regulating soybean photoperiod and flowering time and improving soybean yield. Background technique [0002] Soybean (Glycine max) is one of the most important economic and oil crops in the world, and it is the main source of human vegetable oil and vegetable protein. The nutritional value of soybeans is very high. For example, soybeans contain unique high-quality protein, which can obviously inhibit the rise of blood pressure. The content of unsaturated fatty acids in soybeans is very high, which can promote cholesterol metabolism and reduce cholesterol levels in the body. Soybean is also rich in vitamins, calcium and trace elements needed by the human body, which has a certain effect on improving human immunity, increasing nerve function and vitality (Zhang Haisheng, 2012). In recent years, as people have become more and more aware of the nutritional a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H5/02A01H6/54
CPCC07K14/415C12N15/827C12N15/8261C12N15/8218Y02A40/146
Inventor 孔凡江刘宝辉程群董利东芦思佳
Owner GUANGZHOU UNIVERSITY
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