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Application of corn gene ZmSCL14 in regulating and controlling flowering period of plants

A gene and corn technology, applied in the application field of corn gene ZmSCL14 in regulating the flowering period of plants, to achieve high agricultural application value, slow down the growth rate, and delay the effect of flowering

Active Publication Date: 2021-03-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are still many new functions of new LISCL subfamily gene members waiting to be discovered and studied

Method used

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  • Application of corn gene ZmSCL14 in regulating and controlling flowering period of plants
  • Application of corn gene ZmSCL14 in regulating and controlling flowering period of plants
  • Application of corn gene ZmSCL14 in regulating and controlling flowering period of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: corn ZmSCL14 Gene sequence analysis, cloning and vector construction

[0024] 1. Acquisition of maize ZmSCL14 gene

[0025] Use the corn genome database website gramene to find the gene GRMZM2G368909, named ZmSCL14 , ZmSCL14 The genome sequence is shown in SEQ ID NO.1, the full length is 2421 bp, its CDS sequence is shown in SEQ ID NO.2, the length is 1938 bp, and it encodes 645 amino acids, and the encoded amino acid sequence is shown in SEQ ID NO. 3.

[0026] Will ZmSCL14 Amino acid sequence of Brachypodium distachyon, wheat, Arabidopsis, rice and Sorghum SCL14 protein to construct phylogenetic tree, such as figure 1 As shown, the homology of maize ZmSCL14 protein and sorghum SbSCL14 protein is relatively close.

[0027] 2. Corn ZmSCL14 Gene cloning and vector construction

[0028] 1. Extraction of RNA: Extract the total RNA of corn with the whole gold TRIzon;

[0029] (1) Take fresh plant tissue material and grind it fully in liquid nitrogen, add...

Embodiment 2

[0048] Example 2: Overexpression of Zm SCL14 Screening of Transgenic Brachypodium distachyon Positive Seedlings

[0049] 1. Brachypodium distachyon callus infection and tissue culture

[0050] (1) Configuration of solution and medium:

[0051] Infection solution formula (1L system): 10×Macro MS Salts 100mL; 100×Micro MS Salts 10μL; 200×Fe-EDTA 5mL; 100×M5 Vitamin 10mL; sucrose 30g; glucose 30g; 5.5, sterilized at 121°C for 20 minutes and stored in the refrigerator.

[0052] CIM medium formula (1L system): 10×Macro MS Salts 100mL; 100×Micro MS Salts 10mL; 200×Fe-EDTA 5mL; 100×M5 Vitamins 10mL; CuSO4 (8mg / mL) 7.5mL; 2,4-D ( 1mg / mL) 2.5mL; MES 0.5g; sucrose 30g; inositol 100mg; The bacteria bag is sealed for future use.

[0053] Germination medium formula (1L system): 10×Macro MS Salts 100mL; 100×Micro MS Salts 10mL; 200×Fe-EDTA 5mL; 100×M5 Vitamins 10mL; sucrose 30g; inositol 100mg; MES 0.5g; hydrolyzed casein 500mg; Kinetin (kinin KT 1mg / mL) 400μL; Phytagel 3g; dilute to...

Embodiment 3

[0069] Example 3: Overexpression of Zm SCL14 Phenotypic Analysis of Transgenic Lines

[0070] 1. The homozygous T0 generation strain obtained in Example 2 and the differentiated wild-type Brachypodium distachyon T0 generation strain (control group, WT) were cultivated for seedlings, and numbered when transplanting the seedlings, according to the sequence of numbers Take a single plant for population analysis. If more than 40% of the single plants show the same phenotype, it is considered that the phenotype is a representative phenotype. From this, two representative transgenic lines were screened, numbered OE2 and OE3. ;

[0071]2. Count the flowering time and plant height of the homozygous plants of the T2 generation: the flowering time is the number of days from the germination of the T2 generation Brachypodium distachyda seeds under light conditions, using wet filter paper to dew (similar to wheat, two panicles) There are awns on the lemma of the panicle of Brachypodium,...

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Abstract

The invention discloses an application of a corn gene ZmSCL14 in regulation and control of a flowering period of a plant. The nucleotide sequence of the corn gene ZmSCL14 is as shown in SEQ ID NO. 1.According to the invention, a recombinant overexpression vector is obtained by utilizing the existing plant genetic engineering technology, then brachypodium distachyon callus is transformed by utilizing an agrobacterium infection transformation method, and a homozygous brachypodium distachyon plant of the overexpression ZmSCL14 gene is obtained through tissue culture, identification and screening. Compared with a wild type plant growing under the same condition, the brachypodium distachyon over-expressing the ZmSCL14 gene shows a late flowering phenotype, delays blooming of the brachypodium distachyon, can slow down the growth speed of the brachypodium distachyon plant and participates in regulating and controlling expression of blooming-related genes, so that the ZmSCL14 gene can be applied to cultivation of late flowers or dwarf varieties, and high agricultural application values are realized.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to corn gene engineering ZmSCL14 Application in regulating flowering period of plants. Background technique [0002] The life cycle process of plants needs to go through different developmental stages, the two most important stages are: the production of leaves and the completion of biomass accumulation, which is called the vegetative growth stage; the formation of flower organs, the completion of pollination and the production of seeds, which are One phase is called the reproductive growth phase. The transition of plants from the vegetative growth stage to the reproductive growth stage is often referred to as the floral transition. The occurrence of floral transition requires the joint action of environmental signals and growth signals, such as the length of sunshine, temperature, and plant growth age. The research on the regulation mechanism of plant flowering...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/827C12N15/8261
Inventor 葛磊安海龙马斌王晓冬李雪王敬丹冯宇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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