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Method for producing hybrid seeds by use of pepper genic male sterile line and restorer line

A technology of male sterility and hybrid seed production, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems affecting pepper yield, quality and stress resistance, high seed production cost, gene variation and degradation, etc. problems, to achieve the effects of ease of commercial application, stable seed production site, yield and quality assurance

Inactive Publication Date: 2010-05-19
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods of applying hybrid advantage of pepper in production mainly include: using the direct grouping method of artificial emasculation pollination, the production cost of seeds is high, and the purity is difficult to guarantee; The production of these species has begun to appear, but they all need to be planted once a year. Both the male sterile line and the restorer line need to be multiplied, which increases the cost of seed production; the genetic variation and degeneration of the parents will inevitably occur during the breeding process, resulting in The reduction of hybrid vigor affects the yield, quality and stress resistance of pepper

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Utilize dry pepper type capsicum male sterile dual-purpose line 2003A (commercially available) to produce the method for capsicum hybrid generation seed, comprise the following steps

[0020] 1. In the first year, pepper hybridization experiments were carried out in Yuanmou County, Yunnan Province. Peppers in this area can be overwintered and cultivated. The lowest temperature in winter is ≥ 15°C. The light is sufficient and the water source is convenient. The male parent is the market-bought restorer line 9104, and the female parent is Commercially available nuclear male sterile line 2003A, the ratio of male parent to female parent is 1:8, and the male parent and female parent are planted separately and contiguously. From the bud stage to the early flowering stage, about 50% of the fertile plants in the GMS line 2003A were removed, and the hybrid plants in the male restorer line 9104 were removed, and hybridization was carried out by conventional artificial pollination ...

Embodiment 2

[0026] Method for Producing Hybrid First Generation Seed of Capsicum by Utilizing Capsicum Genic Male Sterile Line 0908A

[0027] This example is basically the same as Example 1, and the only difference is that the used capsicum genic male sterile dual-purpose line is a commercially available line pepper type, and the planting density of the capsicum genic male sterile dual-purpose line is smaller than the dry pepper type, Mu planted 2500 plants, and the restoration line used was commercially available 2001. Isolation adopted gauze isolation. The pruning of the plants was on December 15th, and watered once every 30 days after pruning. One germinates and only repeats the steps of 2 and 3 two steps once, that is, the continuous production period of hybrid capsicum seeds is 3 years in total.

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Abstract

The invention provides a method for producing hybrid seeds by use of pepper genic male sterile line and restorer line. The method comprises the following steps: 1, planting a pepper genic male sterile AB line and a restorer line in an area or a greenhouse where pepper plants are capable of overwintering cultivation respectively, taking sterile plants of the genic male AB line as female parent and taking the restorer line as male parent to perform hybridization so as to produce hybrid seeds; 2, pruning aerial parts of the sterile plants and the restorer line plants after the harvest of hybrid seeds in winter and then performing overwintering cultivation; 3, pruning and tidying dormant buds of the overwintering plants after the dormant buds sprout next year, taking the sterile plants as the female parent and taking the restorer line as the male parent to perform hybridization during florescence so as to produce hybrid seeds; and 4, repeating a step 2 and a step 3 three to four times. The method can continually harvest pepper hybrid seeds in a plurality of years after seeding once in a year, saves labor and time for hybrid seed production, is low in cost and long in heterosis-holding time, capable of guaranteeing the yield and quality of hybrid peppers and convenient for commercial application, and has important significance for popularizing the technology of utilizing pepper heterosis.

Description

technical field [0001] The invention relates to the technical field of vegetable hybrid seed production, in particular to a method for hybrid seed production using capsicum genital male sterile dual-purpose lines. Background technique [0002] The utilization of hybrid vigor in pepper is one of the effective ways to increase the yield, quality and stress resistance of pepper. Studies at home and abroad have shown that the yield increase of the first generation of pepper hybrids is generally 30-50%. However, peppers are characterized by continuous flowering and fruiting, long flowering periods, and scattered flowers. Therefore, the research and utilization of hybrid vigor is more difficult than the concentrated flowering of rice and corn. The crop is much larger as a result. There are three determinants of heterosis utilization, one is the strength of hybrid vigor, the other is the cost of hybrids, and the third is the difficulty of the seed production process. [0003] At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 文锦芬
Owner KUNMING UNIV OF SCI & TECH
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