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Soybean transcription active protein GmPHD6, and coding gene and application thereof

A protein and gene technology, which is applied to the soybean transcriptionally active protein GmPHD6 and its encoding gene and application fields, can solve the problem of lack of protein function research and achieve the effect of improving yield

Active Publication Date: 2013-05-15
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PHD-like proteins were discovered for the first time in Arabidopsis, but there are few functional studies of this kind of proteins in plants

Method used

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  • Soybean transcription active protein GmPHD6, and coding gene and application thereof
  • Soybean transcription active protein GmPHD6, and coding gene and application thereof
  • Soybean transcription active protein GmPHD6, and coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, the screening of soybean GmPDH6 and its coding gene and the cloning of its cDNA

[0055] 1. Acquisition of soybean GmPDH6 and its coding gene

[0056] Using the soybean drought-resistant variety Jindou 23 (JD23) and the drought-sensitive variety Huibuzhi (HBZ, provided by Liu Xueyi, a researcher at the Institute of Economics and Research, Shanxi Academy of Agricultural Sciences) as materials, more than 60 differentially expressed genes were screened by cDNA-AFLP. Among them is a 296bp fragment, which was shown by BLAST soybean EST database to be a zinc finger protein containing a PHD domain. During the comparison, it was found that there were homologous sequences of this gene in the dry and salt cDNA libraries of multiple species. Six members of this family were obtained by comparison in the soybean EST database, named GmPHD1-6 respectively. The gene GmPHD6 has the nucleotide sequence of sequence 1 in the sequence listing and encodes a protein with the am...

Embodiment 2

[0072] Example 2, Functional identification of GmPHD6 protein

[0073] 1. Obtaining GmPHD6 transgenic hairy roots and GmPHD6-RNAi transgenic hairy roots

[0074] 1. Establishment of Agrobacterium rhizogenes-mediated transgenic root system method

[0075] The Agrobacterium rhizogenes infection method was carried out according to the method of Attila Kereszt et al. (Attila Kereszt, et al., Agrobacterium rhizogenes-mediaded transformation of soybean to study of root biology, Nature Protocols, 2007, 2(4), 549-552).

[0076]The specific operation is as follows:

[0077] 1) First, pBI121 containing the GUS gene (Datla RS, Hammerlindl JK, Panchuk B, PelcherLE.Keller W, Modified binary plant transformation vectors with the wild-typegene encoding NPTII, Gene.1992Dec 15; 122(2): 383-4 , the public can obtain from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences), and introduced into Agrobacterium rhizogenes K599 by electric shock method to obtain recomb...

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Abstract

The invention discloses a soybean transcription active protein GmPHD6, and a coding gene and application thereof. The soybean PHD transcription factor is: (a) a protein composed of an amino acid sequence shown as Sequence 2 in a sequence table; or (b) a protein which is derived from the Sequence 2, is formed by the amino acid sequence shown as the Sequence 2 in the sequence table through the substitution and / or deletion and / or addition of one or some amino acid residues and is related to stress tolerance of plants. Experiments of the invention prove that the soybean PHD transcription factor GmPHD6 and the coding gene thereof can be used for the cultivation of stress-tolerant plant species, especially stress-tolerant soybean species such as salt-tolerant and drought-tolerant soybeans.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a soybean transcriptional active protein GmPHD6 and its coding gene and application. Background technique [0002] Changes in physical and chemical factors in the environment, such as drought, salinity, low temperature and other stress factors have an important impact on the growth and development of plants, and in severe cases will cause large-scale crop yield reduction. Therefore, cultivating crops with high stress tolerance is the main focus of planting. one of the goals. At present, the application of genetic engineering technology for breeding has become one of the important methods to improve crop stress tolerance. Higher plant cells have multiple pathways to respond to various stresses in the environment, and transcription factors play a role in regulating the expression of stress tolerance-related effector genes. Many types of transcription factors related to plant stress t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
CPCY02A40/135
Inventor 陈受宜张劲松韦伟张玉芹哈达张万科马彪林晴
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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