Protein related to flowering time of plant, and coding gene and application thereof
A technology of flowering time and protein, applied in the field of protein and its encoding gene and application, can solve the problem of slow progress in the improvement of photoperiod response sensitivity
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Embodiment 1
[0043] Embodiment 1. Acquisition and expression of protein GmFULa related to flowering time and its coding gene
[0044] 1. Acquisition of the flowering time-related protein GmFULa and its coding gene
[0045] This example uses the method of homologous cloning, by designing and synthesizing degenerate primers, using the reverse transcription cDNA of soybean total RNA as a template, and obtaining an EST fragment of soybean through PCR amplification technology; and according to the EST fragment, design specificity Primers were used to clone the full-length cDNA of GmFULa by using RACE technology (Rapid amplification cDNA ends, cDNA end rapid amplification technology).
[0046] 1. Acquisition and verification of EST sequence
[0047] The photoperiod-sensitive soybean variety Zigong Dongdou (Han Tianfu, Gai Junyi, Wang Jinling, Zhou Dongxing, 1998, Discovery of soybean flowering reversal phenomenon, Journal of Crops (02): 168-171) was used as the experimental material, and a shor...
Embodiment 2
[0068] Embodiment 2, the application of GmFULa in promoting plant flowering time ahead of schedule
[0069] 1. Construction of recombinant expression vector pTF101-GmFULa containing GmFULa
[0070] The recombinant vector p3301m-GmFULa is a recombinant vector for expressing the gene by inserting the GmFULa gene (nucleotides 1-732 from the 5' end of sequence 1 in the sequence listing) into the expression vector p3301m.
[0071] Preparation of expression vector p3301m: pBI121 (purchased from Beijing Baierdi Biotechnology Co., Ltd.) and pCAMBIA3301 (purchased from Australia CAMBIA Company) were double-digested with HindⅢ and EcoRI, respectively, and the 3kb digestion product of pBI121 was connected with the 11256bp digestion product of pCAMBIA3301 The product, the vector p3301m was obtained.
[0072] 2. Acquisition of recombinant Agrobacterium
[0073] Take 1 μg of pTF101-GmFULa prepared in the above step 1 to transform Agrobacterium tumefaciens Gv3101 (purchased from Tiangen Bi...
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