Protein related to flowering time of plant, and coding gene and application thereof

A technology of flowering time and protein, applied in the field of protein and its encoding gene and application, can solve the problem of slow progress in the improvement of photoperiod response sensitivity

Inactive Publication Date: 2015-06-17
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the cultivation of soybean varieties by conventional breeding methods has achieved great results, the progress in photoperiod response sensitivity improvement is relatively slow. At present, th

Method used

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  • Protein related to flowering time of plant, and coding gene and application thereof
  • Protein related to flowering time of plant, and coding gene and application thereof
  • Protein related to flowering time of plant, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1. Acquisition and expression of protein GmFULa related to flowering time and its coding gene

[0044] 1. Acquisition of the flowering time-related protein GmFULa and its coding gene

[0045] This example uses the method of homologous cloning, by designing and synthesizing degenerate primers, using the reverse transcription cDNA of soybean total RNA as a template, and obtaining an EST fragment of soybean through PCR amplification technology; and according to the EST fragment, design specificity Primers were used to clone the full-length cDNA of GmFULa by using RACE technology (Rapid amplification cDNA ends, cDNA end rapid amplification technology).

[0046] 1. Acquisition and verification of EST sequence

[0047] The photoperiod-sensitive soybean variety Zigong Dongdou (Han Tianfu, Gai Junyi, Wang Jinling, Zhou Dongxing, 1998, Discovery of soybean flowering reversal phenomenon, Journal of Crops (02): 168-171) was used as the experimental material, and a shor...

Embodiment 2

[0068] Embodiment 2, the application of GmFULa in promoting plant flowering time ahead of schedule

[0069] 1. Construction of recombinant expression vector pTF101-GmFULa containing GmFULa

[0070] The recombinant vector p3301m-GmFULa is a recombinant vector for expressing the gene by inserting the GmFULa gene (nucleotides 1-732 from the 5' end of sequence 1 in the sequence listing) into the expression vector p3301m.

[0071] Preparation of expression vector p3301m: pBI121 (purchased from Beijing Baierdi Biotechnology Co., Ltd.) and pCAMBIA3301 (purchased from Australia CAMBIA Company) were double-digested with HindⅢ and EcoRI, respectively, and the 3kb digestion product of pBI121 was connected with the 11256bp digestion product of pCAMBIA3301 The product, the vector p3301m was obtained.

[0072] 2. Acquisition of recombinant Agrobacterium

[0073] Take 1 μg of pTF101-GmFULa prepared in the above step 1 to transform Agrobacterium tumefaciens Gv3101 (purchased from Tiangen Bi...

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Abstract

The invention discloses a protein related to flowering time of a plant, and a coding gene and application thereof. The protein provided by the invention is protein 1) or 2), wherein 1) is a protein composed of an amino acid sequence as shown in a sequence 2 in a sequence table, and 2) is a protein related to the flowering time of the plants and derived from the sequence 2 through substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence as shown in the sequence 2 in the sequence table. Experimental results of the invention prove that a gene GmFULa is found in soybean and overexpressed in arabidopsis so as to obtain a, and the flowering time of the transgenic plant is earlier than the flowering time of a non-transgenic wild plant; thus, it is observed that the gene or the protein expressed by the gene plays a role in promoting earlier flowering of plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein related to plant flowering time, its coding gene and its application. Background technique [0002] Soybean is the most important oil crop and high-protein food crop in the world. In my country, soybean is one of the four major food crops, which plays a very important role in ensuring national food security, improving urban and rural people's lives and increasing farmers' income. Soybean is a typical short-day crop. The suitable planting range of a single variety is mostly between 1-1.5 latitudes. The introduction of soybeans between different latitudes will lead to early or delayed flowering time and maturity due to changes in the length of sunshine, resulting in decreased yield and even grains. No income. Therefore, cultivating widely-adapted soybean varieties that are relatively insensitive to photoperiod has always been an important goal of soybean breeding. Although...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N15/11A01H5/00
CPCC07K14/415C12N15/827
Inventor 韩天富贾贞岳岩磊蒋炳军高小伟费志宏孙洪波吴存祥孙石侯文胜
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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