5 results about "Plant genomics" patented technology

The invention relates to the technical field of crossing of bioinformatics, artificial intelligence and plant genomics, and discloses a plant single cell gene expression prediction method, system, device and medium. A plant single cell gene expression prediction model realizes dynamic feature fusion of a DNA sequence and chromatin accessibility signals through a gated cross attention mechanism; the problem that a traditional single-mode model cannot model regulation and control dynamic association is effectively solved, and the result interpretability is enhanced; a hybrid expert system and a load balancing design are adopted to significantly improve the recognition capability of the model for rare cell types, and a decoupling prediction head design supports efficient transfer learning; a DNA long sequence processing mechanism and nucleosome scale feature coding ensure cross-species compatibility; an end-to-end automatic process and a dynamic parameter optimization framework greatly improve the practicability; a'prediction-verification 'closed-loop support system can be constructed for molecular breeding, and high-precision and interpretable prediction of plant single-cell gene expression is realized.

Provided herein are highly transformable maize plants, referred to as HI-NA plants, and methods of their production and use. A HI-NA plant, as disclosed herein, is homozygous for a loss-of-function mutant allele in the patatin-like phospholipase A2α (MATL) gene and at least heterozygous for one or more QTL and / or gene alleles that are responsible for increased haploid induction and / or transformation frequency in plants. A HI-NA plant, as disclosed herein, may also have a cytotype A background, which may render it highly transformable. Also provided are methods of producing I-NA plants and methods of using a HI-NA plant for editing plant genomic DNA.

The invention relates to the technical field of biology, in particular to a method for regulating and controlling the methylation level of a plant genome DNA specific region by using a Cas12i system. The invention provides a fusion protein. The fusion protein sequentially comprises a first nuclear localization signal peptide, a methylation modification structural domain, a connecting peptide, a nuclease activity-free Cas12i protein and a second nuclear localization signal peptide from an N terminal to a C terminal, the methylation modification structural domain is OsSUVH2 protein capable of up-regulating the methylation level or a functional active fragment of the OsSUVH2 protein; the amino acid sequence of the OsSUVH2 protein is as shown in SEQ ID NO: 1; the amino acid sequence of the nuclease activity-free Cas12i protein is as shown in SEQ ID NO: 3. The invention also provides a CRISPR-dCas12i system constructed on the basis of the fusion protein. According to the CRISPR-dCas12i system, the methylation modification efficiency of the plant genome DNA is remarkably improved.

The invention discloses a plant genome DNA rapid crude extract and an extraction method based on the crude extract. The DNA crude extract is prepared from the following components: 60 to 150 mM of Tris-HCl and 5 to 12 mM of EDTA (Ethylene Diamine Tetraacetic Acid), and the pH (Potential of Hydrogen) of the DNA crude extract is 7.5 to 8.5. The extraction method based on the crude extract comprises the following steps: adding a plant tissue sample and a DNA extracting solution into a centrifugal tube or a deep-hole plate, grinding the sample, and carrying out warm bath on the sample to obtain the crude extract DNA. The coarsely extracted DNA can be directly used for PCR amplification without dilution. According to the DNA crude extraction method disclosed by the invention, the crude extraction DNA can be obtained only by adding liquid once, high-temperature treatment is not needed, sample pollution possibly caused by multiple times of liquid adding and high-temperature treatment is avoided, and the operation is simple, convenient, rapid and efficient.

The invention relates to the technical field of plant genome DNA extraction, in particular to a safe, non-toxic and rapid plant genome DNA extraction method which comprises the following steps: step 1, selecting fresh plant leaves as a sample, placing the sample in liquid nitrogen for rapid freezing, and then fully grinding the sample in the liquid nitrogen into fibrous powder; step 2, rapidly transferring 100mg of the ground fibrous powder into a centrifugal tube filled with 400mu L of Buffer mLP1 and 5mu L of DNAseA (deoxyribonucleic acid sease) in advance; 3, the plant genome DNA extraction kit is subjected to column dividing, program selecting and running, the plant genome DNA extraction method is matched with the plant genome DNA extraction kit, genome nucleic acid can be conveniently, rapidly and easily obtained from plant samples, operation is easy, multiple samples can be extracted at a time, rapid diagnosis is achieved, safety and non-toxicity are achieved, and the method is suitable for large-scale popularization and application. The vacancy of the automatic high-throughput plant sample genome extraction kit in the current market is filled up.