Quick extraction method of plant genomic DNA (Deoxyribonucleic Acid)

Abstract

The invention discloses a quick extraction method of plant genomic DNA (Deoxyribonucleic Acid). The quick extraction method is characterized by comprising the following steps of (1), taking a plant tissue sample, adding liquid nitrogen, grinding the plant tissue sample into powder and collecting into a centrifugal tube, and the like. SDS (Sodium Dodecyl Sulfonate) in a Buffer A in the quick extraction method can be used for cracking a cell membrane to denature protein; a Buffer B can act with the SDS; impurities of the protein, a polysaccharide and the like can be effectively removed; moreover, PVP40 (Polyvinyl Pyrrolidone 40) in the Buffer A can be used for effectively removing substances of the polysaccharide and polyphenol in plant tissue; the purity of the DNA is improved; the completeness of the DNA is enabled to be better; the quick extraction method can be directly used for a PCR (Polymerase Chain Reaction) and various enzyme digestion reaction; meanwhile, the quick extraction method does not need to use organic solvents of phenol chloroform and the like in the entire extraction process; therefore, steps of ethanol precipitation and the like do not need to be carried out; the extraction method provided by the invention, compared with a conventional extraction method, can be used for more quickly, simply and conveniently extracting the DNA; the operation, on a single sample, of the quick extraction method can be completed within 1 hour and the extraction time is greatly shortened.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapidly extracting plant genome DNA. Background technique [0002] The extraction of plant genomic DNA is the basis for in-depth research on molecular biology of various plants, and the quality of the extracted DNA is the main factor affecting the success or failure of downstream molecular biology experiments. [0003] There are many plant DNA extraction methods reported in the literature, among which the most widely used plant genomic DNA extraction methods are the traditional SDS method and CTAB method. [0004] CTAB is a cationic detergent that dissolves cell membranes and forms a complex with nucleic acids. This complex is soluble in a high-salt environment. Extraction with organic solvents (phenol, chloroform) can effectively remove impurities such as phenols, polysaccharides, and proteins, and the nucleic acid can be separated by adding ethanol or isopropanol for ...

AI Technical Summary

Problems solved by technology

[0006] However, the traditional SDS method and CTAB method have disadvantages such as cumbersome operation, long time-consuming, use of toxic reagents, and unstable quality of extracted DNA during the DNA extraction process.

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