4 results about "Sensitive-plant" patented technology

The invention discloses an InDel molecular marker for detecting black spot resistance of broccoli, a method and application, and relates to the field of molecular biology. The site is located at 56724071bp-56724089bp of a broccoli chromosome 5, and the site has insertion / deletion variation of 18bp basic groups (the nucleotide sequence is as shown in SEQ ID NO. 1). When the site has a 18bp basic group (the sequence of an amplification product is shown as SEQ ID NO.2), the broccoli is a black spot resistant plant; otherwise, the plants are black spot sensitive plants. The invention also discloses a pair of specific primers for detecting the InDel molecular marker, and the nucleotide sequences of the specific primers are respectively shown as SEQ ID NO. 4 and SEQ ID NO. 5. The InDel molecular marker can rapidly and accurately detect the black spot resistance of broccoli germplasm resources at low cost, does not need field inoculation identification, can be used for early screening of broccoli black spot resistance varieties and molecular marker-assisted breeding, significantly improves the breeding efficiency, and shortens the breeding period.

The invention discloses a plant insect-resistant related protein OsGSK2 as well as a coding gene and application thereof. The protein provided by the invention is the following protein: (a) an amino acid sequence as shown in SEQ ID NO.2; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of the SEQ ID NO.2, is related to plant insect resistance and is derived from the SEQ ID NO.2. The plant insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving the expression of the protein coding gene, so that insect-resistant transgenic plants can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The application discloses an anti-herbicide Echinochloa crusgalli glutathione-S-transferase gene EcGSTT1, and the nucleotide sequence is SEQ ID NO:1, or a sequence with not less than 80% homology compared with SEQ ID NO:1. The application introduces the resistance gene glutathione-S-transferase gene EcGSTT1 from Echinochloa crusgalli into plants sensitive to high-efficiency fluazifop, so that the tolerance of the sensitive plants to high-efficiency fluazifop and other AOPP herbicides is significantly enhanced, thereby making the herbicide originally having a sterilization effect on gramineous crops and unable to be safely used in gramineous crop fields be able to be used for precise prevention and control of gramineous weeds in the fields of transgenic crops into which the resistance gene is introduced. The application provides a new gene for cultivating new germplasm of anti-herbicide crops, so as to expand the application range of AOPP herbicides and enrich the gene resource reserves of anti-herbicide crops in China.