Method for identifying cymbidium rapidly

An orchid and plant technology, applied in the field of plant identification, can solve problems such as unclassified

Inactive Publication Date: 2015-12-02
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At the same time, with the rise of orchid leaf art appreciation in many provinces in southern China after the 1980s, Japanese and Korean orchid flower circles often explained the categories of orchid leaf art, but as a non-mainstream classification method, no relevant systematic classification appeared
At present, there is no systematic and comprehensive report on the taxonomy of lotus petal orchid species

Method used

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  • Method for identifying cymbidium rapidly
  • Method for identifying cymbidium rapidly
  • Method for identifying cymbidium rapidly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] 1 test material

[0153] The 31 samples tested were all wild orchids in Yunnan Province. All the materials were introduced and cultivated in the orchid garden of the Flower Institute of the College of Garden and Horticulture, Yunnan Agricultural University. There were 9 non-Orchid samples including Orchid and 22 Orchid samples, and a total of 31 samples were used as samples (see Table 1).

[0154] Table 1 Flower name and collection location

[0155] .

[0156] 2 test method

[0157] 2.1 Extraction of orchid genome DNA

[0158] The DNA extraction method was based on the CTAB method for plant genomic DNA.

[0159] 1) The extraction solution, tips, and centrifuge tubes used to extract DAN need to be autoclaved at 121°C (about 1.1kg / cm) for 20 minutes in an autoclave.

[0160] 2) Take 50㎎ fresh leaves and grind them with liquid nitrogen, put them in a 1.5ml centrifuge tube after grinding, add preheated

[0161] 600 μL of 2×CTAB buffer (100 mM Tris-Hcl, pH=8.0; 20 mM...

Embodiment 2

[0309] 1 test material

[0310] The 37 samples tested were all wild orchids in Yunnan Province, of which 14 samples belonged to the subgenus Jianlan, 5 samples belonged to the subgenus Orchid, 11 samples belonged to the subgenus Dahua, and 7 samples belonged to the family Orchidaceae. Genus type (see figure and table number for specific experimental materials). All the materials were introduced and cultivated in the orchid garden in the greenhouse of the Flower Institute of the College of Landscape and Horticulture, Yunnan Agricultural University. The Chinese and Latin scientific names of the samples refer to the nomenclature given by Chen Xinqi (1999) in Flora of China and Chen Xinqi et al. (2003) in "Correction of some taxa in the genus Orchid" in "Journal of Plant Taxonomy", see Table 2.

[0311] Table 2 Orchids ( Cymbidium ) species and some other genera of Orchidaceae collection resource statistics table

[0312] serial number Name Scientific name stat...

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Abstract

The invention relates to a method for identifying cymbidium rapidly and belongs to the technical field of plant identification. The method is characterized in that cymbidium genomic DNA (deoxyribonucleic acid) is extracted with a CTAB (cetyltrimethyl ammonium bromide) method for plant genomic DNA, a PCR (polymerase chain reaction) product band after amplification is observed through electrophoretic detection according to an RAPD (random amplified polymorphic DNA) amplification reaction of a random primer BS-5 of cymbidium and a PCR amplification reaction of an SCAR (sequence characterized amplified region) labeled primer, and each cymbidium has a specific band about 1070 bp. The method for identifying cymbidium rapidly can be used for detecting a large quantity of species rapidly and has a positive role in sorting of cymbidium species, classified study, species clarification, protection of seed resources, molecular identification, pushing of establishment of an international registration system of cymbidium and the like.

Description

technical field [0001] The invention specifically relates to a method for quickly identifying plants of the genus Orchid, belonging to the technical field of plant identification. Background technique [0002] At the end of the 20th century, the orchid industry developed rapidly. The cultivation group expanded rapidly, and new varieties emerged in an endless stream. In terms of variety classification, the traditional petal type classification is still the main method, and modern emerging technologies and methods have also been tried. Using the quantitative classification method to study the classification of Chinese orchid varieties, the R analysis has preliminarily obtained the characteristic traits of the orchid flower species classification and the characteristic traits of the variety classification, and the Q analysis has obtained the orchid flower species classification boundaries and type classification boundaries. This is the greatest achievement in the classificati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 王玉英李枝林
Owner YUNNAN AGRICULTURAL UNIVERSITY
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