Rive CYP704B2 gene mutant, and molecular identification method and applications thereof

Abstract

The invention provides a rive CYP704B2 gene mutant, and applications thereof, and belongs to the technical field of gene engineering. According to the rive CYP704B2 gene mutant, indica rice 93-11 is subjected to cobalt 60 radiation-induced mutation so as to replace three G bases after the 794th base of rice CYP704B2 gene with one T base; the CYP704B2 gene mutant is named as cyp704B2-3; the nucleotide sequence of the CYP704B2 gene mutant is represented by SEQ ID No.1; it is further confirmed that rice recessive genic male sterility is induced by the CYP704B2 gene mutant; the CYP704B2 gene mutant can be used for culturing transgenic rice with recessive genic male sterility, and possesses great importance in rice genetic resource genetic improvement-breeding. The invention also provides a molecular marked identification method and applications of the rive CYP704B2 gene mutant in breeding.

Description

technical field

[0001] The invention belongs to the field of plant molecular biology, and in particular relates to a rice CYP704B2 gene mutant cyp704B2-3 and its molecular identification method and application. Background technique

[0002] Plant male sterile mutation is a very common phenomenon in nature, at least male sterile mutants have been found in 617 species of 43 families and 162 genera. Genetically, plant male sterility is divided into three categories: nuclear male sterility, cytoplasmic male sterility and nuclear-cytoplasmic interaction male sterility: 1) Nuclear male sterility is produced by nuclear gene mutations, and there are dominant mutations and recessive mutations , There are sporophytic gene mutations and gametophytic gene mutations. Dominant mutations and gametophytic gene mutations can only be inherited through female gametes, recessive mutations can be inherited through both female gametes and male gametes, and follow Mendel's laws. Some sporophytic...

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