3 results about "Outcrossing" patented technology

The invention particularly relates to a breeding method for fertility transformation light-temperature characteristics of a rice photo-thermo sensitive two-line sterile line. The breeding method comprises the steps of S1, selection of a planting environment, S2, two-generation cross breeding, S3, multi-generation breeding, S4, fertility transformation breeding, S5, purification breeding, S6, final selection and propagation expansion and the like. According to the method disclosed by the invention, in the process of breeding the sterile line, the sterile line is planted among generations in three different planting environments in a shuttling manner, and breeding is performed according to the sterile outcrossing characteristic and the fertile self-breeding characteristic of the sterile line by utilizing the fertile or sterile characteristic expression of the sterile line in different environments; the fertility-to-sterility transformation characteristic of the sterile line is directly selected by fully utilizing the light-temperature condition of the fertility transformation light-temperature characteristic of the environment III, so that the hidden danger of two-line hybrid rice seed production is remarkably reduced, and the blindness of indirect speculation of the fertility transformation light-temperature characteristic of the sterile line under the conditions of the environment I and the environment II is avoided; and material resources, manpower and energy consumed by speculation of the character are reduced.

The application discloses a parent-offspring identification primer group and method of Prunus. The parent-offspring identification primer group of Prunus disclosed by the application is composed of 30 single-stranded DNAs shown in sequence 1 to sequence 30 in a sequence list. The primer group and method of the application can identify the genetic relationship of Prunus armeniaca, Prunus dulcis and their offspring, establish a parent-offspring identification system, and are simple to operate. The primer group and method of the application can quickly obtain genetic information of Prunus parents and offspring and determine the source of the offspring, can early determine and retain hybrid offspring between Prunus armeniaca and Prunus dulcis at the seedling stage, which is beneficial to quickly determine the true outcrossing seedling at the seedling stage, thereby removing hybrid offspring caused by natural pollination and eliminating the hybrid offspring at the seedling stage, so as to save land resources, reduce the identification cost in the later period, speed up the breeding process, and also provide a reference for parent-offspring identification of other fruit plants.

The present invention belongs to the technical field of rice molecular genetic breeding, and specifically relates to a molecular marker tightly linked to a QTL for rice long stigma and its application. The present invention discloses a molecular marker Z05 tightly linked to the QTL for rice long stigma, and primers (Z05-F: 5-CTTGGACTGCCGACTGGATT-3; Z05-R: 5-CCTTTCCCCACGCACCTTTA-3) for amplifying molecular marker Z05. Using molecular marker Z05 to select thermosensitive genic male sterile lines with long stigma can improve the selection efficiency of breeding photothermosensitive genic male sterile lines with long stigma and high outcrossing rate, thereby accelerating the breeding process.