4 results about "Outcrossing" patented technology

The invention particularly relates to a breeding method for fertility transformation light-temperature characteristics of a rice photo-thermo sensitive two-line sterile line. The breeding method comprises the steps of S1, selection of a planting environment, S2, two-generation cross breeding, S3, multi-generation breeding, S4, fertility transformation breeding, S5, purification breeding, S6, final selection and propagation expansion and the like. According to the method disclosed by the invention, in the process of breeding the sterile line, the sterile line is planted among generations in three different planting environments in a shuttling manner, and breeding is performed according to the sterile outcrossing characteristic and the fertile self-breeding characteristic of the sterile line by utilizing the fertile or sterile characteristic expression of the sterile line in different environments; the fertility-to-sterility transformation characteristic of the sterile line is directly selected by fully utilizing the light-temperature condition of the fertility transformation light-temperature characteristic of the environment III, so that the hidden danger of two-line hybrid rice seed production is remarkably reduced, and the blindness of indirect speculation of the fertility transformation light-temperature characteristic of the sterile line under the conditions of the environment I and the environment II is avoided; and material resources, manpower and energy consumed by speculation of the character are reduced.

The invention provides an efficient breeding method for feed triticale based on a dominant genic male sterility gene Ms2, and belongs to the technical field of triticale breeding. According to the method, dominant genic male sterility hexaploid triticale is used as a donor parent, an excellent triticale breeding variety or a seedling line is used as a recurrent parent for backcrossing, and a triticale recurrent selection breeding base population is constructed; then constructing a continuously developing triticale recurrent selection breeding population by continuously introducing various excellent germplasms by taking the dominant genic male sterile line of the excellent breeding variety and the seedling head strain as a basic material; meanwhile, continuously improved breeding populations are obtained through continuous selective outcrossing of sterile plants and fertile plants in the populations; excellent fertile plants are selected from the improved population, variety breeding is conducted through a pedigree breeding method, and the excellent new triticale variety is obtained. The breeding method of the triticale broadens the genetic basis of breeding populations, and the obtained excellent triticale has the characteristics of high yield, strong stress tolerance, excellent feeding character and the like.

The application discloses a parent-offspring identification primer group and method of Prunus. The parent-offspring identification primer group of Prunus disclosed by the application is composed of 30 single-stranded DNAs shown in sequence 1 to sequence 30 in a sequence list. The primer group and method of the application can identify the genetic relationship of Prunus armeniaca, Prunus dulcis and their offspring, establish a parent-offspring identification system, and are simple to operate. The primer group and method of the application can quickly obtain genetic information of Prunus parents and offspring and determine the source of the offspring, can early determine and retain hybrid offspring between Prunus armeniaca and Prunus dulcis at the seedling stage, which is beneficial to quickly determine the true outcrossing seedling at the seedling stage, thereby removing hybrid offspring caused by natural pollination and eliminating the hybrid offspring at the seedling stage, so as to save land resources, reduce the identification cost in the later period, speed up the breeding process, and also provide a reference for parent-offspring identification of other fruit plants.

The present invention belongs to the technical field of rice molecular genetic breeding, and specifically relates to a molecular marker tightly linked to a QTL for rice long stigma and its application. The present invention discloses a molecular marker Z05 tightly linked to the QTL for rice long stigma, and primers (Z05-F: 5-CTTGGACTGCCGACTGGATT-3; Z05-R: 5-CCTTTCCCCACGCACCTTTA-3) for amplifying molecular marker Z05. Using molecular marker Z05 to select thermosensitive genic male sterile lines with long stigma can improve the selection efficiency of breeding photothermosensitive genic male sterile lines with long stigma and high outcrossing rate, thereby accelerating the breeding process.