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Increasing hybrid seed production through higher outcrossing rate in cytoplasmic male sterile rice and related materials and methods

A technology for male sterility and male sterility, which is applied in biochemical equipment and methods, botanical equipment and methods, plant regeneration, etc., can solve the problem of high cost of hybrid rice seeds, poor hybrid seed production, and low outcrossing rate question

Inactive Publication Date: 2018-03-09
INT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low outcrossing rate leads to poor hybrid seed production (5-20% seed set rate), which leads to high cost of hybrid rice seeds

Method used

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  • Increasing hybrid seed production through higher outcrossing rate in cytoplasmic male sterile rice and related materials and methods
  • Increasing hybrid seed production through higher outcrossing rate in cytoplasmic male sterile rice and related materials and methods
  • Increasing hybrid seed production through higher outcrossing rate in cytoplasmic male sterile rice and related materials and methods

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0221] According to some exemplary embodiments, the DNA locus is a quantitative trait locus related to stigma length, which may be selected from: qSTGL2-1; qSTGL5-1; qSTGL8-1; qSTGL8-2 and qSTGL11-1. At least one marker of at least one O. longista quantitative trait locus associated with stigma length is selected from the group consisting of: RM110 (qSTGL2-1); RM421 (qSTGL5-1); RM7356 (qSTGL8-1); RM5353 (qSTGL8-1) RM256(qSTGL8-2); RM80(qSTGL8-2); RM590(qSTGL11-1); RM286(qSTGL11-1); RM120(qSTGL11-2) and RM229(qSTGL11-2).

[0222] In another embodiment described herein, at least one Oryza longista quantitative trait locus associated with total stigma and style length may be selected from: qPSTL1-1; qPSTL1-3; and qPSTL11-1. The at least one marker of at least one Oryza longista quantitative trait locus associated with total stigma and style length may be selected from: RM3604 (qPSTL1-1); RM3640 (qPSTL1-3); and RM5997 (qPSTL11-1).

[0223] Exemplary markers for validation of long...

Embodiment 1

[0240] Example 1. Characterization of pistil traits in Oryza species to identify wild species as donors for the transfer of floral traits affecting outcrossing

[0241]To understand the degree of variability in pistil traits, stigma length, stigma width, style length, and total stigma were identified in 47 accessions of Oryza 24 species covering 11 genomes, including cultivated rice and two unrelated plant species and style length (Table 1; Figure 10 ). No data were collected for O. schelcteri as it never flowered under tropical conditions. Cultivars of the O. sativa subspecies japonica have significantly shorter stigmas, styles, and total length than indica cultivars. Among the wild species with the AA genome, Oryza longistamina had significantly longer and wider stigma, style and total length than the other species. At the genome complex level, the Malayan rice (O. ridleyi) complex has significantly longer stigmas than the Asian rice (O. sativa) complex and the medicinal...

Embodiment 2

[0246] Example 2. Development of IR58025B maintainer line with long stigma

[0247] A cross was made between the maintainer line IR58025B and Oryza longistaminata (Accession No. 110404) to transfer the long stigma trait to the IR58025B background. in bc 1 f 1 , for 475 strains of BC from 12 crosses 1 f 1 Plants were evaluated for stigma length and lines with stigma length 50% greater than the backcross parent were selected and backcrossed to produce 46 BC 2 f 1 hybrid seeds. in bc 2 f 1 , 1653 strains of BC from 46 crosses were evaluated 2 f 1 plants and backcross with the respective backcross parents to produce 34 BC 3 f 1 seed. in bc 1 f 2 In , 944 plants were evaluated and 45 plants with longer stigmas were selected. in bc 2 f 2 In , 1109 plants were evaluated and 98 plants with longer stigmas were selected. Stigma lengths in different backcross generations ranged from 0.85 to 2.88 mm, while stigma+style lengths ranged from 2.22-4.57 mm (Table 2).

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Abstract

Methods for increasing hybrid seed production are provided. Increased hybrid seed production is achieved through higher outcrossing rates in cytoplasmic male sterile (CMS) lines of rice by introgressing the long stigma trait of Oryza longistaminata. CMS lines having higher outcrossing rates capable of high hybrid seed set are also provided.

Description

Background technique [0001] Rice is the staple food for more than half of the world's population, providing more than 20% of the daily caloric intake for more than 3.5 billion people. It is estimated that by 2035, an additional 116 million tons of rice will be needed to feed the world's growing population. [0002] Beginning in the 1940s and 1950s, increased yields gradually replaced the expansion of acreage as the main source of growth in cereal production worldwide. The Green Revolution that took place in the 1940s to the late 1960s saw the development of new farming methods and technologies that significantly improved grain yields, and in the early 1960s the Green Revolution saved several million people. In particular, the rice variety IR8 was developed, which produces more grains per plant when grown under the action of irrigation and fertilizers. Numerous other high-yielding rice lines have been developed since IR8. [0003] Now, the Green Revolution technologies that...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H5/10C12Q1/68
CPCA01H5/10C12Q1/6895C12Q2600/156C12Q2600/13A01H1/045A01H1/021A01H1/023A01H4/008C12N15/8289
Inventor 克施洛德·K·杰纳巴拉姆·马拉迪乔伊·拉莫斯雷纳尔多·迪奥克顿四世里基·维纳拉欧G·D·帕拉哈拉达金中原
Owner INT RICE RES INST
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