Seedless monordica grosvenori and cultivating method thereof
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A cultivation method and technology of Luo Han Guo, applied in horticultural methods, botanical equipment and methods, plant cells, etc., to achieve the effects of high utilization rate of the whole fruit, enhanced matability, and high content
Active Publication Date: 2008-02-13
桂林亦元生现代生物技术有限公司
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Problems solved by technology
So far, people have not conducted research on triploid Luo Han Guo, and the...
Method used
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Embodiment 1
[0020] Set the indoor cultivation temperature at 15-24°C, and perform indoor operations under sterile conditions;
[0021] 1) taking explants of diploid Luo Han Guo female and male plant explants activated by flowering factors, and breeding tissue culture seedlings according to conventional methods;
[0022] 2) Select tissue-cultured subcultured seedlings with strong growth potential, cause wounds in different parts, wrap them tightly with absorbent cotton, drip colchicine solution with a mass concentration of 0.03% on the absorbent cotton for 10 days, and wash with sterile water;
[0023] 3) Cut out different parts of the female and male plants after the induction treatment, and transfer them to the differentiation medium for dark culture for 10 days, then light culture for 20 days, light for 8-12 hours per day, and the light intensity is 1000-2000LX;
[0024] 4) cutting out the stem tips and stem segments of the new shoots grown after the above-mentioned cultivation and tran...
Embodiment 2
[0030] Set the indoor cultivation temperature at 24-32°C, and perform indoor operations under sterile conditions;
[0031] 1) taking explants of diploid Luo Han Guo female and male plant explants activated by flowering factors, and breeding tissue culture seedlings according to conventional methods;
[0032] 2) select the subcultured seedlings for tissue culture, soak their terminal buds with a colchicine solution with a mass concentration of 0.8% for 3 days, and wash them with sterile water;
[0033] 3) Cut out the different parts of the induced female and male plants, and transfer them to the differentiation medium for dark culture for 5 days, then light culture for 20 days, with 12-16 hours of light per day, and the light intensity is 2000-3600LX ;
[0034] 4) Cut out the stem tips and stem segments of the sprouts grown after the above culture and transfer them to the callus culture medium to induce callus production and proliferation, cut the callus into small pieces, and...
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Abstract
The present invention discloses a pipless fructus momordicae and the cultivation method. The cultivation method is: 1) breeding the tissue cultivation seedling with the male and the female ex-plant of diploid fructus momordicae; 2) doubly introducing the chromosome with the tissue-cultivation successive-transfer seedling; 3) performing the differential cultivation with different parts of the male and the female plants after introducing treatment; 4) cutting the stem tip and the stem section of the sprout differentially cultivated to perform the gemmulation cultivation; 5) examining the number of the chromosome of a cultivated entire plant to select the tetraploid plant for performing the root cultivation and the seedling exercise; 6) planting the tetraploid plant and the diploid plant according to the conventional techniques and performing the artificial pollination to get the hybrid seed when in flower; 7) breeding the hybrid seed to be a complete plant and examining the number of the chromosome; selecting the triploid plant to perform the root cultivation and seedling exercise; 8) planting the triploid plant and the diploid plant and performing the artificial pollination on the male diploid plant with the female triploid plant when in flower; the pipless fructus momordicae can be obtained after the fruiting of the female plant.
Description
(1) Technical field: [0001] The invention relates to the field of biotechnology breeding, in particular to seedless Luo Han Guo, and also relates to a cultivation method of the seedless Luo Han Guo. (two) background technology: [0002] Luo Han Guo Siraitiagrosvenororii (Swile) C. Jeffrey is the fruit of the perennial vine of Cucurbitaceae Luo Han Guo. It is unique to my country and has a history of more than 300 years for both medicine and food. Modern research shows that the medicinal active ingredient of Luo Han Guo is mogroside, which has the functions of antitussive and expectorant, two-way regulation of intestinal motility, scavenging free radicals in the human body, inhibiting caries caused by Streptococcus mutans in the oral cavity, protecting the liver and preventing cancer, and lowering serum cholesterol , hypoglycemic, hypolipidemic effect. Mogroside is one of the strongest sweetening substances in the world, among which Mogroside V is 300 times sweeter than sucr...
Claims
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Application Information
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