9results about How to "Efficient cultivation" patented technology

The invention provides a method for efficiently preparing FGF5 homozygous knockout cashmere goats by combining a base editor with a somatic cell cloning technology. And an sgRNA site is designed. An sgRNA expression vector is constructed, the sgRNA expression vector and pCMV-AncBE4max are respectively transferred into fibroblasts of a down producing goat fetus, and the editing efficiency is analyzed. And optimizing an electrotransfection condition, and simultaneously transfecting the screened sgRNA expression vector and pCMV-AncBE4max into a down producing goat fetal fibroblast to obtain the FGF5 homozygous knockout cell strain. And preparing reconstructed embryos and FGF5 homozygous knockout cashmere goats. The average editing efficiency of the sgRNA site disclosed by the invention can reach 64.3 + / -8.1%. The average probability of FGF5 homozygous knockout cells is 69.0%. Wherein the homozygous probability is 58.6% on average. The lambing rate is 7%. The invention provides a theoretical basis and a research basis for cultivating high-yield and high-quality new varieties.

The utility model relates to the technical field of ivy mosses breeding, in particular to a temperature control device for efficient ivy mosses breeding, which comprises a box body, a connecting cavity is arranged at the inner top of the box body, connecting pipes are communicated with the connecting cavity, and a heating cavity and a humidifying cavity are respectively arranged below the connecting cavity; a plurality of electrical bars are mounted in the heating cavity, and an atomizer is mounted at the inner bottom of the humidifying cavity; on one hand, the air inlet fan can send heat of the multiple electric heating bars into the breeding box, so that the temperature in the breeding box can be increased, the temperature in the breeding box can be guaranteed in low-temperature weather, and healthy growth of ivy mosses seedlings is facilitated; on the other hand, the atomizer atomizes the water source in the humidifying cavity to form water mist, and the air inlet fan can send the water mist into the breeding box, so that the humidity in the breeding box can be improved, and healthy growth of the dried ballonflower seedlings is facilitated; according to the design, the temperature and humidity in the breeding box are regulated and controlled, so that the requirements on the temperature and humidity in the growth process of the ivy mosses seedlings are met.

The present invention discloses a method for predicting rosin properties based on infrared spectroscopy, the method comprising: S1, collecting rosin samples from wetland pine wood, dividing the rosin samples into a first rosin sample and a second rosin sample; S2, analyzing the sample components based on the first rosin sample to obtain the rosin components; S3, collecting near-infrared spectral data of the sample based on the second rosin sample to obtain near-infrared spectral data of the rosin; S4, correlating and fitting the rosin components with the near-infrared spectral data to establish a near-infrared correction model for rosin properties; S5, using an external validation set of rosin samples that do not participate in modeling to verify the near-infrared correction model for rosin properties; S6, predicting rosin properties based on the near-infrared correction model for rosin properties to obtain a rosin property prediction result. The present invention improves the efficiency of genetic improvement of resin-producing tree species and promotes the rapid development of forest genetic improvement research.

The utility model discloses an efficient cultivation device for garden landscape aquatic plants, which comprises a hollow-square-shaped floating platform, an aquatic landscape plant cultivation frame is fixedly sleeved in the hollow-square-shaped floating platform, a hollow-square-shaped floating air bag is fixedly embedded on the outer side of the hollow-square-shaped floating platform, a hollow-square-shaped supporting plate is fixedly embedded on the top of the aquatic landscape plant cultivation frame, and a hollow-square-shaped floating air bag is fixedly embedded on the hollow-square-shaped supporting plate. The top of the concentric-square-shaped supporting plate is fixedly connected with a concentric-square-shaped top base with the top of an arc-shaped structure. By arranging a series of structures, aquatic plants can be cultivated on a garden landscape water body in a floating supporting mode, blocking is automatically relieved when the wind speed is low, the aquatic plants are automatically blocked and protected on the upper portion when the wind speed exceeds a preset value, and the risk that the aquatic plants are impacted and damaged by blown-off branches and other falling objects in strong wind days is effectively avoided; moreover, the upper part of the aquatic plant can be automatically watered at regular time, the timeliness and the automation degree of watering are improved, and the cultivation effect is improved.

The utility model discloses a detachable moss cultivating and sowing integrated device which comprises a ball body which is formed by splicing and used for planting, a plurality of round holes are evenly formed in the surface of the ball body, moss is planted in each round hole, a water injection mechanism is coaxially arranged on the top of the ball body, and a water outlet is formed in the top of the ball body. According to the device, the moss is planted on the surface of the sphere, water and nutrients are continuously supplied through the internal structure, high-quality cultivation of the moss is guaranteed, and the moss can be conveniently and rapidly cultivated; the device can be used for sowing spores when rolling, is simple in structure, attractive, practical, easy to operate and suitable for moss cultivation and sowing of moss spores in terrains such as side slopes, can achieve good cultivation and sowing effects, and solves the problems that in traditional moss cultivation, the process is tedious, no adaptive cultivation and sowing device exists, and efficiency is low.

The utility model discloses a plant seedling cultivation device, which relates to the technical field of tea cultivation, and comprises a cultivation box with an opening at the top, a display window is arranged on the side surface of the cultivation box, scales are arranged on the display window, a lifting plate capable of moving up and down is arranged at the top in the cultivation box, and the lifting plate is arranged on the top of the cultivation box. A supporting plate is fixed to the lower end face of the lifting plate in parallel, a vertical planting barrel is fixed to the lifting plate and the supporting plate jointly, the bottom of the planting barrel is open, a nutrient solution in the cultivation box enters the planting barrel, and a driving mechanism for driving the lifting plate and the supporting plate to do lifting motion is arranged at the bottom in the cultivation box. The device is reasonable in structure, the relative water level between plants in the planting cylinder and water and nutrient solutions in the cultivation box can be accurately adjusted by adjusting the horizontal position of the lifting plate in the cultivation box, and compared with the prior art, the device is easy to operate, more accurate in adjustment and good in use effect.

The invention relates to a grafting technology for efficiently cultivating high-quality propagule nursery stocks of Machilus pauhoi. From the middle ten days of December to the middle ten days of January of the next year, selecting a 2-year-old excellent machilus pauhoi seedling which grows robustly and is free of plant diseases and insect pests and has the ground diameter of more than 1.2 cm as a stock, and taking a current-year-old branchlet which grows normally, is free of plant diseases and insect pests, has the diameter of 0.4-0.5 cm and is lignified, sap starts to flow and the phloem is easy to peel off at the middle upper part of other excellent scion-picking seed trees as a scion; cutting the rootstock and cutting the scion, inserting the scion into the rootstock notch, and binding and fixing the rootstock notch and the scion cutting surface; after grafting is carried out for 20-25 days, when axillary buds of the scions grow to 1.8-2.2 cm, binding bands for binding the scions are removed; removing the binding band for binding the rootstock 45 days after grafting; cutting off coppice shoots on the rootstock after new twigs of the scions grow to 20-25 cm. The method has the beneficial effects that the grafting survival rate of the Machilus pauhoi can be improved, the excellent hereditary characteristics of the stock and the fruiting seed tree can be fused by utilizing the grafting technology, and the high-quality propagule nursery stock of the Machilus pauhoi can be efficiently and creatively cultivated.

The utility model relates to the technical field of seedling raising, and discloses a seedling raising device for peach tree planting, which comprises a cultivation box, a box door is hinged on the side wall of the cultivation box, a handle is fixedly arranged on the box door, a light supplementing lamp is fixedly arranged in the cultivation box, an adjusting mechanism is arranged on the cultivation box, and the adjusting mechanism is arranged on the cultivation box. The adjusting mechanism comprises a U-shaped piece, and one end of the U-shaped piece is fixedly installed on the inner wall of the cultivation box. According to the seedling raising device for peach tree planting, in order to efficiently cultivate plant seedlings, the adjusting mechanism is arranged, an asynchronous motor is started firstly, a driving rod and a driven rod are driven through a rotating shaft, a tray is made to rotate at a small angle in a unified mode in cooperation with a linkage rod, and therefore a seedling raising plate is made to rotate, and the seedling raising plate is made to rotate more stably in cooperation with a fastener and a baffle; meanwhile, soil is effectively prevented from being scattered, the seedlings can receive proper illumination at different stages by adjusting the angle of the tray, and then efficient cultivation of the plant seedlings is achieved.

This utility model relates to a sweet potato aeroponic cultivation device. The cultivation system includes a cultivation box, LED lights, solar panels, and a battery. The cultivation box forms a trapezoidal platform and is equipped with a cultivation trough and a slide rail. The nutrient solution supply system includes water pipes, atomizing nozzles, a nutrient solution tank, water and fertilizer pipes, valves, a flow detector, a fertilizer tank, a phosphate tank, a weight detector, a flow pump, a stirrer, and a controller. The water pipes are slidably mounted on the slide rails, and the atomizing nozzles are installed on the water pipes. The nutrient solution tank is connected to the atomizing nozzles. Valves and flow detectors are installed on the water and fertilizer pipes. The fertilizer tank and phosphate tank are respectively connected to the nutrient solution tank through the water and fertilizer pipes, and weight detectors are installed at the bottom of each. The flow detector, weight detector, and valves are respectively connected to the controller. It is easy to operate, facilitates control of the nutrient solution ratio and supply, maintains sufficient light, reduces power consumption, has high space utilization, adapts to various environments, saves time and labor, ensures high-efficiency production, and promotes high-efficiency cultivation.