The invention provides a method for distinguishing production places of flat tea based on stable isotope proportion difference. The method is characterized by mainly comprising the following steps: (1) collecting samples and establishing a modeling sample set; (2) detecting stable isotopes, wherein determined indexes are 13C/12C, 15N/14N, 18O/16O, D/1H, 111Cd/113Cd, 206Pb/207Pb, 207Pb/208Pb and 88Sr/86Sr; (3) carrying out data standard processing and sample assignment to obtain variables Z(C), Z(N), Z(O), Z(D), Z(Cd), Z(206Pb/207Pb), Z(207Pb/208Pb) and Z(Sr); (4) establishing a decision-making tree analyzing model; (5) judging unknown samples. With the adoption of the method provided by the invention, the quality safety of tea leaves can be met, and the faking of the tea leaves can be effectively prevented; the production places are distinguished through decision-making tree analysis, the accurate distinguishing rate of a return test reaches 91.35 percent, the accuracy of an external test reaches 92 percent, and the model is reliable.