98 results about "Genetic selection" patented technology

A self-cleaving element for use in bioseparations has been derived from a naturally occurring, 43 kDa protein splicing element (intein) through a combination of protein engineering and random mutagenesis. A mini-intein (18 kDa) previously engineered for reduced size had compromised activity and was therefore subjected to random mutagenesis and genetic selection. In one selection a mini-intein was isolated with restored splicing activity, while in another, a mutant was isolated with enhanced, pH-sensitive C-terminal cleavage activity. The enhanced cleavage mutant has utility in affinity fusion-based protein purification. The enhanced splicing mutant has utility in purification of proteins such as toxic proteins, for example, by inactivation with the intein in a specific region and controllable splicing. These mutants also provide new insights into the structural and functional roles of some conserved residues in protein splicing. Thus, disclosed and claimed are: a genetic system and self-cleaving inteins therefrom; bioseparations employing same; protein purification by inactivation with inteins in specific regions and controllable intein splicing; methods for determining critical, generalizable residues for varying intein activity; and products.

The invention discloses a hybrid intelligent optimization method and belongs to the technical field of artificial intelligence and data mining. A genetic optimization algorithm and a bacterial foraging optimization algorithm are organically combined by the hybrid intelligent optimization method. First, a preliminary superior solution is obtained through the breadth searching capacity of the genetic optimization algorithm and serves as an initial bacterial population in the posterior bacterial foraging algorithm, by fully utilizing chemotaxis, duplicating and dispersing operation of the bacterial foraging algorithm, excellent individuals are generated continuously, and finally the solution converges towards the optimal solution. On the basis of the technical scheme, further improvements are made in the four aspects of genetic selection operators, optimal joint pints, bacterial chemotaxis and duplicating operation. Compared with the prior art, the convergence speed and the convergence precision of an optimal solution set can be improved, and the hybrid intelligent optimization method is more extensive in applicability.

The invention discloses SNP markers linked with the color of husked millet. The SNP markers comprise a marker HC-06-436 and a marker HC-06-250, wherein the marker HC-06-436 is located at the site 34024436 bp of the sixth chromosome of millet and the marker HC-06-250 is located at the site 34035250 bp of the sixth chromosome of millet. The invention also discloses amplimers for acquiring the SNP markers and application of the SNP markers. The SNP markers linked with the color of husked millet and the amplimers thereof can realize good typing of the color of husked millet and detection of SNP differences, are applicable to molecular marker-aided breeding of the character of the color of husked millet, provide molecular assistive technology support for early identification of the character of the color of husked millet and for screening and breeding, and are of important theoretical and practical guidance significance to acceleration of genetic breeding and improvement of millet varieties.

The invention relates to a molecular assisted technology for genetic breeding, in particular to a microsatellite marking method applicable to parentage determination of hard clam. The microsatellite marking method comprises four steps of obtaining microsatellite loci source, designing a primer, optimizing the primer and establishing a microsatellite marker parentage determination system, and the specific process is as follows: firstly using a bead enriching library method to screen microsatellite sequences, and using a microsatellite retrieval software to carry out rapid separation of microsatellite fragments; designing a primer on a microsatellite repeated flanking sequence, optimizing the primer and leading the primer to become a microsatellite marker; then carrying out a comprehensive evaluation on repeatability, stability and polymorphism information content value during the amplification process of the microsatellite marker, and determining the microsatellite markers therein as the microsatellite parentage determination system of the hard clam for differentiation among different parentages of the hard clam or identification and determination among individuals. According to the invention, a molecular marking method for genetic breeding of the hard clam is provided.

A method for selecting host cells with an improved ability to recombinantly overexpress a target protein; the host cells thus generated and their use. The invention also provides a curing method to remove plasmids from host cell lines.

The present invention relates to methods of selecting for highly productive clones of E. coli for the production of plasmid DNA comprising measuring the frequency of IS1 transposon insertional mutagenesis within either the plasmid or genomic DNA of transformed clonal subtypes. An increase in IS1 insertional mutagenesis is correlated with clonal subtypes likely to exhibit a low specific productivity. The PCR-based, genetic selection assays disclosed herein are amenable to high throughput analysis, reducing the time to identify highly productive clones capable of cultivating large quantities of plasmid DNA on an industrial scale.

The invention belongs to the technical field of aquatic breeding, and particularly relates to a growth-related molecular marker in a C-type scavenger receptor gene (LvSRC) of Litopenaeusvannamei and application thereof to genetic breeding of the Litopenaeusvannamei. A batch of markers related to shrimp growth traits are positioned in the genomic level through genome-wide association analysis, a single nucleotide polymorphism (SNP) marker (LvSRC-211-G/T) positioned in a coding region of the LvSRC gene is further screened through gene annotation and by a candidate gene association analysis method, and the genotype of the marker is significantly correlated to the shrimp growth traits (P is smaller than 10<-6>). The marker is positioned at a 211bp position on the LvSRC gene and belongs to G/Ttype mutation, and the GT genotype of the marker is a distinct growth-dominant genotype. The marker provided by the invention can be used as the shrimp breeding molecular marker for assisting in genetic selection of the shrimp growth traits.

The invention provides a composition used in insect targeted gene knock-in, which includes a single nickase or an encoding polynucleotide thereof; a recombinant factor or an encoding polynucleotide thereof; and optionally, a donor DNA of an exogenous gene. The invention also provides a corresponding gene knock-in method and a method for preparing transgenic insects. The composition and the method in the invention can achieve high-effective homologous recombination integration in insect cells and meanwhile can avoid the defects in gene knock-in technologies in the prior art. The composition also can stably and high-effectively achieve homologous recombination integration of a fragment being more than 10 kb in length in the insect cells, so that the composition can be used for transferring a genetic selection marker into the insect cells, preferably a visible genetic selection marker, which is convenient to determine strains of transgenic insects.

The invention discloses a greedy genetic algorithm-based pot seedling thin planting and transplantation path optimization method. Health information of pot seedlings in a transplantation hole tray of a greenhouse pot seedling thin planting and transplantation machine is obtained through machine vision, and healthy seedling hole positions in the transplantation hole tray and empty hole positions in a target hole tray are subjected to label coding respectively; a greedy genetic selection principle is that the empty holes in the target hole tray are partitioned by column for carrying out current path optimization of a local genetic algorithm; the coding of a column of empty holes in the target hole tray is integrated with the coding of unplanned seedling holes in the transplantation hole tray, random path coding is generated, an initial population of the local genetic algorithm is formed, the selection, crossing, variation and reinsertion operations are circularly carried out until preset convergence generations, and individuals with maximum population fitness serve as local optimal paths; and the successive columns of planned local optimal paths are combined to generate a thin planting and transplantation path of the whole target hole tray. According to the method, an optimal path for greenhouse pot seedling thin planting and transplantation can be generated, the transplantation efficiency can be improved, and the real-time planning requirements of a control system are met.

The invention discloses a constructing method of micro-satellite marked scallop, which comprises the following steps: extracting DNA; connecting adapter cut by enzyme; preaugumenting before crossing; crossing to enrich micro-satellite sequence through micro-satellite marked by biotin and SAV magnetic bead; obtaining DNA segment with enriched micro-satellite sequence; augumenting the segment; constructing the enriched library with enriched micro-satellite sequence; utilizing PCR to sieve the micro-satellite sequence; obtaining the result of DNA sequence; comparing the quality of segment with enriched micro-satellite sequence; designing the primer of micro-satellite sequence; adopting PAGE to detect and sieve the augumentation, polymorphism and stability of micro-satellite; statisticallizing genetic parameter of corresponding group.

The invention discloses an SNP marker relevant to the plant height character of millet, detection primers for the SNP marker, and applications. For the SNP marker relevant to the plant height character of millet, the SNP marker is located in the bin markers of the 30703512bp to the 30784296bp of the first chromosome, and the SNP marker is in close linkage with the plant height of millet. The SNP marker relevant to the plant height character of millet can be used for the molecular marker-assisted breeding for the plant height character of millet, through the detection of the SNP marker, the plant height of millet can be predicated, a scientific basis is provided for realizing the early identification or screening breeding for the plant height character of millet, and great theory and practice guiding significance is achieved for accelerating the genetic breeding or improvement process of the varieties of millet. The primer pair for detecting the SNP marker provided by the invention canrealize typing on the plant height of millet and detect the differences of SNP expression.

The present invention concerns a tester protein for identifying and/or monitoring protease activity in a cellular assay suitable for high throughput screenings by growth selection, wherein the tester polypeptide is a non-regulatory protein carrying a protease cleavage sequence. Upon co-expression of the protease recognizing said cleavage sequence the tester protein is inactivated, which influences the growth and/or survival of the host cells under the chosen conditions. However, in the presence of protease inhibitor the growth phenotype is reversed. The system can be used to identify proteases, protease inhibitors, and protease cleavage sites.

The present invention relates to a genes and autoimmunity. More specifically, the invention relates to the identification of genes that are associated with Systemic Lupus Erythematosus (SLE) in children and/or adults. Also disclosed are methods for making and using the genetic selection tool used to identify SLE associated genes, and their uses in diagnosis and treatment of the disease.

In accordance with the present invention, there are provided selection systems and methods for screening for agents that control splicing of inteins in their native host protein (extein) or in homologous exteins. Specifically, there are provided positive genetic selection systems for the screening of agents which inhibit or activate protein splicing which comprise: a host cell containing a chromosomal gene encoding either a drug-resistant form of a target enzyme or a wild-type target enzyme, and a plasmid-borne gene encoding either a drug-sensitive form of the target enzyme, which is dominantly cytotoxic upon interaction with the drug, or a dominantly cytotoxic form of the target enzyme. In these systems the plasmid-borne gene contains an intein, and the inhibition or activation of splicing of the dominant cytotoxic form of the target enzyme by a given reagent results in the survival or death of the host cell. More specifically, positive genetic selection systems which utilize the M. xenopi GyrA intein or M. tuberculosis DnaB helicase intein are provided. Similar reporter systems utilizing native or homologous exteins and systems utilizing controllable inteins are provided, as are methods of controlling in vivo expression of proteins by modulating protein splicing with inhibiting or activating agents, and methods of controlling the delivery of proteinaceous drugs in vivo by modulating protein splicing.

The present invention relates to the field of genetic selection, where particular genetic traits or loci combinations are sought in a progeny resulting from genetic breeding. The invention provides genetic engineering solutions to select or counter-select the occurrence of genetic events.

Methods and compositions are provided for altering the DNA recognition and cleavage characteristics of an endonuclease without prior knowledge of the endonuclease's three-dimensional structure and/or amino acid residues responsible for activity and/or specificity. Methods include subjecting a mutagenized endonuclease gene library to a genetic selection in prokaryotic cells which tolerate the expression of mutated endonuclease and where the endonuclease is active and determining the altered recognition-site specificity for the endonuclease.

The invention provides a pattern recognition method and device based on a convolutional neural network and computer equipment. The pattern recognition method comprises the following steps: determininga plurality of initial neural units; taking the plurality of initial neural units as basic genes of a genetic algorithm to perform iterative computation, wherein genetic selection is performed according to the accuracy of pattern recognition of chromosome individuals in an iterative process, and the higher the accuracy of pattern recognition of the chromosome individuals is, the lower the probability that the chromosome individuals are mutated and crossed; when the iteration result meets a preset termination condition, constructing a target convolutional neural network according to the chromosome individuals of which the accuracy meets a first preset threshold; and inputting the to-be-identified object into the target convolutional neural network to obtain a mode identification result corresponding to the to-be-identified object, wherein the pattern recognition result can be stored in a block chain. According to the pattern recognition method and device, the workload of workers in theprocess of constructing the convolutional neural network model can be reduced, and the efficiency of pattern recognition is improved.

The invention discloses an SNP marker associated with foxtail millet flag leaf length characters and detection primers and application thereof. According to the SNP marker associated with the foxtailmillet flag leaf length characters, the SNP marker is located between the bin marker of the 39221591th bp and the bin marker of the 39480016th bp of a second chromosome, and the SNP marker is closelylinked with the foxtail millet flag leaf length. The SNP marker associated with the foxtail millet flag leaf length characters can be used for molecular mark assisted breeding of the foxtail millet flag leaf length characters, through detection of the SNP marker, the foxtail millet flag leaf length can be predicted, a scientific basis is provided for achieving early identification or breeding of the foxtail millet flag leaf length characters, and it is of an important theory and practice guiding significance to accelerate genetic breeding or the improvement process of the foxtail millet varieties. By means of the primer pair for detecting the SNP marker, the foxtail millet flag leaf length can be somatotyped, and difference of SNP expressions can be detected.

The invention discloses an SNP marker related to the growth rate character of basa fish, detection primers and application. The SNP marker related to the growth rate character of the basa fish is a sequence as shown in SeqID No.1. The 301st basic group from the 5' terminal of the sequence is G or T. The SNP marker related to the growth rate character of the basa fish can be used for molecular marker-assisted breeding of the growth rate character of the basa fish; the growth rate of the basa fish can be predicted through detection of the SNP marker; a scientific basis is provided for early identification or screening breeding of the growth rate character of the basa fish; and the SNP marker has important theoretical and practical guiding significance for accelerating the genetic breeding or improvement process of basa fish varieties. The primer pair for detecting the SNP marker can be used for typing the genotype of the growth rate of the basa fish and detecting the difference of SNP expression.

The invention relates to the technical field of biology, in particular to a sweet clover hairy root transformation method. By adopting the method to establish a sweet clover hairy root transformation system, transgenic hairy roots can be obtained about two weeks after seedling root cutting and infection, which is quicker and more convenient than transgenic plants obtained by an agrobacterium tumefaciens mediated genetic transformation system, and the occurrence rate and the transformation rate of the obtained hairy roots are 78 +/-2% and 70.8 +/-4.2% respectively; and the relative average expression quantity of the overexpressed MaROP6 is 36.87 times that of a control group. Therefore, the transformation of the hairy roots of the sweet clover by utilizing the agrobacterium rhizogenes can be used as a biotechnological means for studying the function of the nodulation gene of the sweet clover, and the agrobacterium rhizogenes can be possibly popularized to the development of other fields including stress resistance mechanism study, so that a foundation is laid for the verification and genetic breeding of the gene function of the sweet clover.