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SNP marker relevant to plant height character of millet, detection primers for SNP marker, and applications

A high-character, labeling technology, used in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problem of unreported research on the development and application of SNP molecular markers.

Active Publication Date: 2018-10-12
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP marker relevant to plant height character of millet, detection primers for SNP marker, and applications
  • SNP marker relevant to plant height character of millet, detection primers for SNP marker, and applications
  • SNP marker relevant to plant height character of millet, detection primers for SNP marker, and applications

Examples

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Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the materials of the male parent "Ji Zhanggu No. 1" and the female parent "A2" of millet, the F1 generation materials of the two hybrids, and 441 materials of the F2 population of the 13th generation of selfing, that is, recombinant inbred lines (Recombinant inbred lines , abbreviated as RILs). All materials were planted in Zhangjiakou, Hebei, and the data of traits such as plant height were recorded and sorted. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the data of plant height traits, the associated SNP markers were obtained, and the SNP markers were verified by clone sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each sample individual, a total of 444 samples, including 441 RILs, 2 parents, and 1 F1 individu...

Embodiment 2

[0058] Embodiment 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select the amplified products with normal primer amplification and the compounded predicted size of the PCR amplification products for gel cutting recovery and sequencing, and select the labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials. Using the F1 and F2 generation materials as templates, carry out PCR amplification of SNP marker primers and sequencing of the results, and select marker primers that are consistent with genotyping and phenotypic traits.

[0060] First, according to the sequencing results after PCR product recovery, the markers for the di...

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Abstract

The invention discloses an SNP marker relevant to the plant height character of millet, detection primers for the SNP marker, and applications. For the SNP marker relevant to the plant height character of millet, the SNP marker is located in the bin markers of the 30703512bp to the 30784296bp of the first chromosome, and the SNP marker is in close linkage with the plant height of millet. The SNP marker relevant to the plant height character of millet can be used for the molecular marker-assisted breeding for the plant height character of millet, through the detection of the SNP marker, the plant height of millet can be predicated, a scientific basis is provided for realizing the early identification or screening breeding for the plant height character of millet, and great theory and practice guiding significance is achieved for accelerating the genetic breeding or improvement process of the varieties of millet. The primer pair for detecting the SNP marker provided by the invention canrealize typing on the plant height of millet and detect the differences of SNP expression.

Description

technical field [0001] The present application relates to the technical field of millet breeding, in particular to a SNP marker related to the plant height traits of millet, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Plant height and panicle length are important factors affecting millet yield. It is of great significance to study the genes related to the control of millet plant height and other yield traits and their position and function for guiding millet genetic breeding. [0004] Single nucleotide polymorphism (singlenucleotide polymorphism, SNP) is a DNA sequence polymorphism caused by the variation of a single nucleotide base at the genome level. It h...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 赵治海宋国亮魏玮王晓明张耕耘王德权李双东裴晶晶李欣儒
Owner ZHANGJIAKOU ACAD OF AGRI SCI
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