Molecular marker closely linked to leaf angle gene of paddy, detection method and application

A molecular marker, technology of millet leaves, applied in the determination/inspection of microorganisms, biochemical equipment and methods, introduction of foreign genetic material using vectors, etc. problems, to achieve the effect of speeding up the process of genetic selection and improvement

Active Publication Date: 2021-06-29
SHENZHEN BGI AGRI & CYCLE ECONOMIC TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the millet varieties used in production are scattered plant types, which have low utilization rate of light resources, and the utilization efficiency of light energy is less than 1%.

Method used

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  • Molecular marker closely linked to leaf angle gene of paddy, detection method and application
  • Molecular marker closely linked to leaf angle gene of paddy, detection method and application

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Experimental program
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Effect test

Embodiment

[0042] 1. Construction of millet F2 genetic population and investigation of traits

[0043] The male parent is An 12-L262: a millet variety cultivated by Anyang Academy of Agricultural Sciences, with green leaves and good plant shape traits. At the heading stage, the three leaves are upshooting, the leaf angle is compact, and the leaf angle of the lower leaves gradually becomes larger. Short, spindle-shaped ears, small ears, fertile.

[0044] The female parent is A2 male sterile line: the millet male sterile line cultivated by Zhangjiakou Academy of Agricultural Sciences, which is currently widely used in hybrid millet. Compact, long setae, club-shaped ears, large ears, partly sterile.

[0045] The F1 generation with non-compact leaf angle was obtained by crossing the male parent and the female parent, and a total of 400 F2 generation plants were obtained by selfing of the F1 generation. The leaf angle traits of 400 F2 individual plants were analyzed, and it was found that 3...

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Abstract

The application discloses a molecular marker closely linked to a leaf angle gene of paddy, a detection method and application. The molecular marker closely linked to the leaf angle gene of the paddy, disclosed by the application, comprises a sequence represented by SEQ ID No. 1 and is closely linked to a leaf angle compact type gene of the paddy. According to the molecular marker closely linked to the leaf angle gene of the paddy, disclosed by the application, a genomic DNA sequence is related to the leaf angle compact type gene, so that the establishing of a paddy-molecular-marker-assisting breeding system is facilitated; and A genetic close-linked distance between the molecular marker and the leaf angle compact type gene is 2.1cM. The molecular marker disclosed by the application and detection primers thereof can be used for typing leaf angle compact type genes of the paddy, and thus, a molecular-assisting technical support is provided for early identifying and screening breeding of leaf angle compact types of the paddy; and the molecular marker can be applied to assisted breeding of the paddy in a simple, convenient, rapid and high-pass manner and has an important theoretical and practical guiding significance in accelerating a genetic breeding and improving progress of paddy varieties.

Description

technical field [0001] This application relates to the field of millet gene molecular markers, in particular to a molecular marker closely linked with the millet leaf angle gene and its detection method and application. Background technique [0002] Millet is an important food crop originated in my country, and it is also a characteristic drought-resistant crop; the seed germination only needs 26% of its weight in water, while the seed germination of sorghum, wheat and corn requires 40%, 45% and 48% of its weight respectively. moisture. At the same time, millet has higher tolerance to low soil fertility. Millet is an environment-friendly C4 crop and a diploid self-pollinated crop with a genome of about 470M, which is very suitable as a genome research object. [0003] Many crops such as wheat, rice and corn have significantly improved lodging resistance, light energy use efficiency and yield levels by breeding new varieties with ideal plant types. At present, most of the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11C12N15/70C12N1/21
CPCC12Q1/6895C12N15/70C12Q2600/13
Inventor 杜国华邹洪锋李泽桦蔡伟崔巨多常锋侯军亮
Owner SHENZHEN BGI AGRI & CYCLE ECONOMIC TECH
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