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SNP marker related to growth rate character of basa fish, detection primers and application

A growth rate, pangasius technology, applied in SNP markers, detection primers and application fields, can solve the problem of molecular markers to be mined and so on

Inactive Publication Date: 2021-12-17
游兆欣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, molecular markers related to growth traits that can be effectively used in pangasius breeding at this stage still need to be discovered

Method used

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  • SNP marker related to growth rate character of basa fish, detection primers and application
  • SNP marker related to growth rate character of basa fish, detection primers and application
  • SNP marker related to growth rate character of basa fish, detection primers and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 The acquisition of SNP markers related to the growth rate of pangasius

[0040] 1.1 Acquisition of pangasius populations

[0041] The group used was pangasius hatched on June 15, 2017 in a pangasius farm in Lingshui, Hainan. On August 2, 2017, 60,000 fry of the same age were transferred to a 10-acre pond for further breeding. On May 8, 2018, 500 individuals were randomly selected from the pond, and the dorsal fin rays of the cut fish were cleaned and disinfected with 95% ethanol and stored at -20°C for genomic DNA extraction; 500 individuals were previously weighed.

[0042] 1.2 Pangasius genomic DNA extraction

[0043] In this experiment, the conventional phenol-chloroform method was used to extract the genomic DNA in the fin rays of pangasius, and the specific steps were as follows:

[0044] (1) Take 0.3-0.5g of fin rays in a 1.5mL Eppendorf tube, cut into pieces, and dry for 20 minutes on a clean bench;

[0045] (2) After the ethanol is basically vol...

Embodiment 2

[0051] Example 2 Verification and application of SNP markers related to pangasius growth rate

[0052] 2.1 Extract the genomic DNA in the fin rays of pangasius to be tested

[0053] The pangasius to be tested comes from the pangasius group in Example 1, and 200 fishes are randomly selected again, and the genomic DNA is extracted according to the DNA extraction method in Example 1; These 200 tail sharks were weighed.

[0054] 2.2 Amplification of nucleotide fragments containing SNP sites

[0055] According to the SNP site that is significantly correlated with body weight obtained in Example 1, the primer pair that can specifically amplify the SNP site is designed in this experiment. The upstream primer of the primer pair is the sequence shown in Seq ID No.2, and the downstream primer is Seq ID No.2. The sequence shown in ID No.3;

[0056] Seq ID No.2: 5'-CTCCTTGTGTGCGTCCTGGTGTGTCTAA-3'

[0057] Seq ID No. 3: 5'-AAAACATTGCTGTTTGTTATTTAAAA-3'.

[0058] Using the designed pri...

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Abstract

The invention discloses an SNP marker related to the growth rate character of basa fish, detection primers and application. The SNP marker related to the growth rate character of the basa fish is a sequence as shown in SeqID No.1. The 301st basic group from the 5' terminal of the sequence is G or T. The SNP marker related to the growth rate character of the basa fish can be used for molecular marker-assisted breeding of the growth rate character of the basa fish; the growth rate of the basa fish can be predicted through detection of the SNP marker; a scientific basis is provided for early identification or screening breeding of the growth rate character of the basa fish; and the SNP marker has important theoretical and practical guiding significance for accelerating the genetic breeding or improvement process of basa fish varieties. The primer pair for detecting the SNP marker can be used for typing the genotype of the growth rate of the basa fish and detecting the difference of SNP expression.

Description

technical field [0001] The application relates to the technical field of pangasius breeding, in particular to a SNP marker, detection primer and application related to pangasius growth speed traits. Background technique [0002] Pangasius belongs to Siluriformes and Miscanthus genus, and is an important freshwater aquaculture species in Southeast Asian countries. Pangasius has strong adaptability to the water environment and low requirements for breeding techniques. As a growing species, pangasius is currently very popular in the aquatic product market. In 2017, the global production of pangasius was only 1.1 million tons. In 2018, the output reached 2.87 million tons. In 2019, the global pangasius production will exceed 3 million tons. With the small scale of pangasius breeding, the phenomenon of inbreeding of pangasius fingerlings is more prominent, resulting in the degradation of germplasm, mixed species, etc., resulting in the decline of economic traits of pangasius f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 游兆欣
Owner 游兆欣
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