SNP marker related with millet tiller number character and detecting primer and application thereof

A tiller number and marker technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., and can solve the problems that the development and application of SNP molecular markers have not been reported.

Active Publication Date: 2018-10-26
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP marker related with millet tiller number character and detecting primer and application thereof
  • SNP marker related with millet tiller number character and detecting primer and application thereof
  • SNP marker related with millet tiller number character and detecting primer and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the material of the male parent "Jizhanggu No. 1" and the female parent "A2" of millet, the F1 generation material of the cross between the two, and the 441 materials of the F2 population of 13 generations of self-inbred, that is, the recombinant inbred lines (Recombinant inbred lines) , abbreviated RILs). All materials were planted in Zhangjiakou, Hebei Province, and the data of tiller numbers and other traits were recorded and sorted. After degenerate genome resequencing of each material, the alignment of the reference genome was completed, and SNP markers were obtained. By analyzing the tiller number trait data, the SNP markers related to it were obtained, and the SNP markers were verified by clone sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each individual sample, a total of 444 samples, including 441 RILs, 2 parents...

Embodiment 2

[0058] Example 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300bp before and after the SNP site, design and develop SNP marker primers, and perform PCR amplification with the DNA of the male and female parent materials as templates. The amplification products with normal primer amplification and the predicted size of PCR amplification products were selected for gel cutting recovery and sequencing, and labeled primers with SNP site differences in the amplified gene sequences of the male and female parent materials were selected. Using the F1 and F2 generation materials as templates, PCR amplification of SNP marker primers and sequencing results were performed, and marker primers that were consistent with genotyping and related to phenotypic traits were selected.

[0060] First, according to the sequencing results after the recovery of the PCR p...

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Abstract

The invention discloses an SNP marker related with a millet tiller number character and a detecting primer and application thereof. The SNP marker related with the millet tiller number character is located in a bin marker between 48401847bp and 48662086bq in the third chromosome, and the SNP marker is in close linkage with millet tiller number. The SNP marker related with the millet tiller numbercharacter can be used for molecular marker assistant breeding of the millet tiller number character, predict the millet tiller number through SNP mark detection, provide scientific basis for early evaluation or screening breeding of the millet tiller number character, and have important theory and practice guiding significance for accelerating selected genetic breeding or improvement process of millet varieties. A primer pair for detecting the SNP marker can conduct typing on the millet tiller number and detect differences of SNP expression.

Description

technical field [0001] The present application relates to the technical field of millet breeding, in particular to a SNP marker related to the tiller number trait of millet and its detection primer and application. Background technique [0002] my country is the origin of millet and the country with the largest cultivation area and highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest quantity and richest diversity of millet genetic resources. [0003] Tillering is the branching that occurs in grasses such as millet below or near the ground. The effective tiller number has a direct relationship with the number of ears per unit area, and is an important factor affecting millet yield. It is of great significance to study the genes related to the control of yield traits such as tiller number and their location functions, which are of great significance for guiding millet genetics and breedin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 赵治海赵芳范光宇史高雷张耕耘冯小磊张雅莉王德权张文英
Owner ZHANGJIAKOU ACAD OF AGRI SCI
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