SNP marker associated with foxtail millet flag leaf length characters and detection primers and application thereof

A marker and millet technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problems that the development and application of SNP molecular markers have not been reported.

Active Publication Date: 2018-10-26
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP marker associated with foxtail millet flag leaf length characters and detection primers and application thereof
  • SNP marker associated with foxtail millet flag leaf length characters and detection primers and application thereof
  • SNP marker associated with foxtail millet flag leaf length characters and detection primers and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the materials of the male parent "Ji Zhanggu No. 1" and the female parent "A2" of millet, the F1 generation materials of the two hybrids, and 441 materials of the F2 population of the 13th generation of selfing, that is, recombinant inbred lines (Recombinant inbred lines , abbreviated as RILs). The materials were planted in Sanya, Hainan, and the trait data such as flag leaf length were recorded and sorted. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the data of flag leaf length traits, the associated SNP markers were obtained, and the SNP markers were verified by clone sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each sample individual, a total of 444 samples, including 441 RILs, 2 parents, and 1 F1 individua...

Embodiment 2

[0058] Embodiment 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select the amplified products with normal primer amplification and the compounded predicted size of the PCR amplification products for gel cutting recovery and sequencing, and select the labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials. Using the F1 and F2 generation materials as templates, carry out PCR amplification of SNP marker primers and sequencing of the results, and select marker primers that are consistent with genotyping and phenotypic traits.

[0060] First, according to the sequencing results after PCR product recovery, the markers for the di...

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Abstract

The invention discloses an SNP marker associated with foxtail millet flag leaf length characters and detection primers and application thereof. According to the SNP marker associated with the foxtailmillet flag leaf length characters, the SNP marker is located between the bin marker of the 39221591th bp and the bin marker of the 39480016th bp of a second chromosome, and the SNP marker is closelylinked with the foxtail millet flag leaf length. The SNP marker associated with the foxtail millet flag leaf length characters can be used for molecular mark assisted breeding of the foxtail millet flag leaf length characters, through detection of the SNP marker, the foxtail millet flag leaf length can be predicted, a scientific basis is provided for achieving early identification or breeding of the foxtail millet flag leaf length characters, and it is of an important theory and practice guiding significance to accelerate genetic breeding or the improvement process of the foxtail millet varieties. By means of the primer pair for detecting the SNP marker, the foxtail millet flag leaf length can be somatotyped, and difference of SNP expressions can be detected.

Description

technical field [0001] The application relates to the technical field of millet breeding, in particular to a SNP marker related to the flag leaf length trait of millet, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] The flag leaf is the most important photosynthetic organ in the later stage of millet growth, and it is also the main source of carbohydrates in the grain. The area index of flag leaf length and width has a very significant positive correlation with the yield, and is an important factor affecting the yield of millet. Research and Controlling the genes related to yield traits such as flag leaf length and flag leaf width and their position and functio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵治海王晓明李双东赵芳张耕耘范光宇宋国亮裴晶晶张雅莉
Owner ZHANGJIAKOU ACAD OF AGRI SCI
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