SNP marker relevant to stem diameter character of millet, detection primers for SNP marker, and applications

A technology of labeling and millet, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., and can solve the problems of unreported research on the development and application of SNP molecular markers.

Active Publication Date: 2018-10-12
新疆天山小米农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP marker relevant to stem diameter character of millet, detection primers for SNP marker, and applications
  • SNP marker relevant to stem diameter character of millet, detection primers for SNP marker, and applications
  • SNP marker relevant to stem diameter character of millet, detection primers for SNP marker, and applications

Examples

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Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the materials of the male parent "Ji Zhanggu No. 1" and the female parent "A2" of millet, the F1 generation materials of the two hybrids, and 441 materials of the F2 population of the 13th generation of selfing, that is, recombinant inbred lines (Recombinant inbred lines , abbreviated as RILs). Each material was planted in Zhangjiakou, Hebei, and the trait data such as stem diameter were recorded and sorted out. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the data of stem thickness traits, the SNP markers related to them were obtained, and the SNP markers were verified by clone sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each sample individual, a total of 444 samples, including 441 RILs, 2 parents, and 1 F1 i...

Embodiment 2

[0058] Embodiment 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select the amplified products with normal primer amplification and the compounded predicted size of the PCR amplification products for gel cutting recovery and sequencing, and select the labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials. Using the F1 and F2 generation materials as templates, carry out PCR amplification of SNP marker primers and sequencing of the results, and select marker primers that are consistent with genotyping and phenotypic traits.

[0060] First, according to the sequencing results after PCR product recovery, the markers for the di...

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Abstract

The invention discloses an SNP marker relevant to the stem diameter character of millet, detection primers for the SNP marker, and applications. For the SNP marker relevant to the stem diameter character of millet, the SNP marker is located in the bin markers of the 31510348bp to the 32556243bp of the second chromosome, and the SNP marker is in close linkage with the stem diameter of millet. The SNP marker relevant to the stem diameter character of millet can be used for the molecular marker-assisted breeding for the stem diameter character of millet, through the detection of the SNP marker, the stem diameter of millet can be predicated, a scientific basis is provided for realizing the early identification or screening breeding for the stem diameter character of millet, and great theory and practice guiding significance is achieved for accelerating the genetic breeding or improvement process of the varieties of the millet. The primer pair for detecting the SNP marker provided by the invention can realize typing on the stem diameter of millet and detect the differences of SNP expression.

Description

technical field [0001] The present application relates to the technical field of millet breeding, in particular to a SNP marker related to the stem thickness trait of millet, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] The stem of millet refers to the backbone of the aboveground part of the millet plant. The stem is connected to the root, and the water and minerals absorbed by the root are transported up to various vegetative organs through the xylem, and the photosynthetic products are transported down through the phloem, which has the function of transporting Functions of nutrients, photosynthesis, storage of nutrients and reproduction. Therefore, indicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵治海史高雷王峰魏玮张耕耘李双东张文英裴晶晶邱风仓
Owner 新疆天山小米农业科技有限公司
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