The invention relates to a medium being able to optimize virus replication. The medium contains a fusion protein E with the concentration of 100-1000nM, and a cell line is a host cell line over-expressing the fusion protein E gene. The fusion protein E can improve the TCID50 tilter of various viruses by 1-3 lg units and improve the HA tilter by 2-4 lg2 units, the various viruses comprise coronavirus, paramyxovirus, orthomyxovirus, reovirus and herpes virus, the fusion protein E has no cytotoxicity in a prescribed dosage, breaks a boundary between DNA viruses and RNA viruses, and also breaks the boundary between viruses with envelopes and viruses without envelopes. The fusion protein E obtained through a prokaryotic expression system has a high expression efficiency, and can be easily purified, the one-step purification efficiency can reach 85% or above, and the final concentration of the protein can reach 3mg/ml. The fusion protein E can be directly added to the virus medium in order to improve the tilter of various virus vaccines, so the medium is simple and fast. The medium has great influences and meanings in the production, learning and research of the virus subject.