5 results about "Host cell line" patented technology

The present disclosure relates to the introduction of expression constructs encoding different gene products, such as proteins or nucleic acids, into a host cell line containing a plurality of docking sites for insertion of nucleic acid constructs in defined ratios, and the production of proteins requiring the expression of at least two gene products.

Described herein is a protein display selection method which uncouples a protein of interest (POI) library from the display selection system. Display of the POI can be achieved by forming a covalent bond between the POI and the anchor protein post expression either by enzymatic protein ligation (e.g. SpyLigase, SnoopLigase, sortase, butelase, peptiligase etc.) or by spontaneous covalent bond formation (e.g. SpyTag / SpyCatcher, SnoopTag / SnoopCatcher, etc.). The POI library is fused to a tethering sequence, for example SpyTag, at the C-terminus of the POI which then forms a covalent bond to a capture sequence found on an anchor protein, for example, the SpyCatcher-fused anchor protein, e.g., a SpyCatcher-geneIII protein (SpyCatcher-pIII) fusion, for the most common form of phage display. Nucleic acid constructs, host cell systems and methods of producing the protein display systems are also provided.

Disclosed herein are host cell lines and producer cell lines derived from a HeLaS3 parent cell line, and methods of producing the host cell lines and producer cell lines.

Disclosed are a multi-vector expression system encoding precursor mRNA molecules and precursor mRNA trans-splicing molecules (PTM), and a cultured eukaryotic recombinant host cell comprising the system. Furthermore, disclosed are a method for recombinantly expressing one or more target proteins in a cultured eukaryotic host cell line, and a method for screening a eukaryotic host cell recombinantly expressing a target protein in vitro, which employ the multi-vector expression system of the present application.

The present invention relates to the introduction of expression constructs encoding different gene products such as proteins at defined ratios into host cell lines containing multiple dock sites for insertion of the nucleic acid construct, and to the production of proteins that require expression of at least two gene products such as an enzyme and a substrate for the enzyme.