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Improved cell lines having reduced expression of NOCR and use thereof

A cell and host cell technology, applied in the field of cell culture, can solve problems such as reduced protein production, achieve the effects of reducing production costs, increasing protein production, and accelerating drug development

Inactive Publication Date: 2012-04-11
BOEHRINGER INGELHEIM INT GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it will lead to a reduction in the total protein yield of the industrial production process

Method used

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  • Improved cell lines having reduced expression of NOCR and use thereof
  • Improved cell lines having reduced expression of NOCR and use thereof
  • Improved cell lines having reduced expression of NOCR and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0239] Example 1: Knockdown of TIP-5

[0240] In order to engineer cells to increase the synthesis of recombinant proteins, we determined whether the decrease in the number of silenced rRNA genes promotes the synthesis of pre45S rRNA, and therefore also stimulates ribosomal biosynthesis and increases the number of ribosomes that can be translated. Therefore, we use RNA interference to knock down the expression of TIP5, and use shRNA / miRNA sequences specific to two different regions of TIP5 (TIP5-1 and TIP5-2) to construct NIH / 3T3 for stably transgene expression shRNA or for expression of miRNA HEK293T and CHO-K1. A stable cell line expressing mixed shRNA and miRNA sequences was used as a control. There are two reasons for generating stable cell lines instead of transient transfection using plasmids expressing shRNA-TIP5 or miRNA-TIP5 sequences. First, the loss of epigenetic genetic markers such as CpG methylation inhibition is a passive mechanism that requires multiple cell div...

Embodiment 2

[0241] Example 2: Knockdown of TIP-5 leads to a decrease in rDNA methylation

[0242] In NIH / 3T3 cells, about 40% to 50% of rRNA genes contain CpG methylation sequences and are transcriptionally silent. The rDNA promoter sequence and CpG density between humans, mice and Chinese hamsters are significantly different. The human rDNA promoter contains 23 CpGs, while the mouse and Chinese hamsters contain 3 and 8 CpGs respectively ( figure 2 A-C). To confirm that knockdown of TIP5 can affect rDNA silencing, we determined the rDNA methylation level by measuring the amount of meCpG in the CCGG sequence. Genomic DNA was digested with HpaII, and the resistance to digestion (ie CpG methylation) was determined by quantitative real-time PCR using primers containing HpaII sequence (CCGG). In all cell lines knocked down TIP5, most of the rRNA genes in the promoter region of CpG methylation decreased, confirming that TIP5 plays a key role in promoting rDNA silencing ( figure 2 ).

[0243] No...

Embodiment 3

[0244] Example 3: Increased rRNA levels in cells knocked down TIP-5

[0245] To determine whether the reduction in the number of silenced genes will affect the amount of rRNA transcripts, we used qRT-PCR ( image 3 A) And by incorporation of BrUTP in vivo ( image 3 B), the 45S pre-rRNA synthesis is measured. As expected, both NIH / 3T3 and HEK293T cells with reduced TIP5 produced more rRNA than the control cell line measured in both analyses.

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Abstract

The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. The invention further concerns a method of producing proteins using the cells generated by the described method.

Description

Technical field [0001] The present invention relates to the field of cell culture technology. It is about a production host cell line with increased expression of ribosomal RNA (rRNA). This increase is achieved by reducing the expression of NoCR protein (especially TIP-5). These cell lines have improved secretion and growth characteristics than control cell lines. Background of the invention [0002] Screening out mammalian cell lines for efficient producers remains a major challenge in the biomedical manufacturing industry. The process from DNA to product translation is the main bottleneck that limits the specific productivity of mammalian production cell lines. Cells can up-regulate the rate of protein synthesis by increasing the translation efficiency of existing ribosomes or by generating new ribosomes (ribosome biosynthesis). Since about 80% of the total nuclear transcription is used to synthesize ribosomal RNA (rRNA), ribosomal biosynthesis is one of the main metabolic a...

Claims

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Application Information

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IPC IPC(8): C12N15/11
CPCC12N15/111C12N15/85C12N2310/14C12N15/1136C12P21/00C12N15/113C12N15/63C12N15/67C12N5/10C12N15/11
Inventor L.弗洛林B.埃南克尔M.弗瑟尼格H.考夫曼R.桑托罗
Owner BOEHRINGER INGELHEIM INT GMBH
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