Improved cell lines having reduced expression of NOCR and use thereof

A cell and host cell technology, applied in the field of cell culture, can solve problems such as reduced protein production, achieve the effects of reducing production costs, increasing protein production, and accelerating drug development
CN102414320AInactive Publication Date: 2012-04-11BOEHRINGER INGELHEIM INT GMBH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BOEHRINGER INGELHEIM INT GMBH
Publication Date
2012-04-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. The invention further concerns a method of producing proteins using the cells generated by the described method.
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Description

Technical field

[0001] The present invention relates to the field of cell culture technology. It is about a production host cell line with increased expression of ribosomal RNA (rRNA). This increase is achieved by reducing the expression of NoCR protein (especially TIP-5). These cell lines have improved secretion and growth characteristics than control cell lines. Background of the invention

[0002] Screening out mammalian cell lines for efficient producers remains a major challenge in the biomedical manufacturing industry. The process from DNA to product translation is the main bottleneck that limits the specific productivity of mammalian production cell lines. Cells can up-regulate the rate of protein synthesis by increasing the translation efficiency of existing ribosomes or by generating new ribosomes (ribosome biosynthesis). Since about 80% of the total nuclear transcription is used to synthesize ribosomal RNA (rRNA), ribosomal biosynthesis is one of the main metabolic a...

Claims

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