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Triple vaccine of pig transmissible gastroenteritis, pig epidemic diarrhea and pig rotavirus

A technology for porcine epidemic diarrhea and porcine rotavirus, applied in the field of triple inactivated vaccine and triple vaccine, can solve the problems of unsatisfactory treatment effect, mixed infection, rapid onset, etc., and achieve good safety and no drug residue , short half-life effect

Inactive Publication Date: 2009-07-29
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is difficult to distinguish these three diseases clinically, and sometimes it is a mixed infection 【3】
Because antibiotics are mainly used to treat the disease clinically, the therapeutic effect is not very satisfactory, and the onset of these three diseases is rapid. After diarrhea occurs, most piglets are dehydrated due to diarrhea, and will soon die due to exhaustion.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Cultivation of virus-inoculated cell lines

[0029] The ST cells that had grown into a 90% monolayer were inoculated with TGEV, adsorbed at 37°C for 1 hour, and added maintenance solution. Cultivate at 37°C for 48-72 hours, and harvest when the CPE reaches above 75%. Store at -20°C.

[0030] The Vero cells that had grown into a 90% monolayer were inoculated with PEDV, adsorbed at 37°C for 1 hour, and added maintenance solution. Cultivate at 37°C for 48-72 hours, and harvest when the CPE reaches above 75%. Store at -20°C.

[0031] Mac145 cells grown into 90% monolayer were inoculated with porcine rotavirus, adsorbed at 37°C for 1 hour, and added maintenance solution. Cultivate at 37°C for 48-72 hours, and harvest when the CPE reaches above 75%. Store at -20°C.

Embodiment 2

[0032] Example 2: Inoculation and cultivation of virus

[0033] Divide each virus into 10 -6.5 TCID 50Mix in equal amounts and inactivate with formaldehyde at 37°C for 24 hours. Add oil adjuvant to make water-oil-water vaccine. The entire process control and finished product inspection are carried out in strict accordance with the "Procedures" standard.

Embodiment 3

[0034] Embodiment 3: the curative effect of triple vaccine

[0035] The vaccine was immunized with different doses of 10 50-day-old hogs / group, 2mL / head, 3mL / head. ELISA was used to measure the changes of immune antibody levels.

[0036] 4mL / head dose to immunize 5 sows 30 days before delivery. ELISA Determination of Antibody Level Changes of Sow Active Immunization and Passive Immunity of Piglets 【4】 .

[0037] After the laboratory experiment, conduct a field experiment to investigate the protection rate and morbidity rate of vaccine application.

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PUM

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Abstract

The invention provides a method for preparing triple vaccine for preventing porcine transmissible gastroenteritis, porcine epidemic diarrhea and porcine rotavirus. The method comprises the following steps: inoculating a host-cell line with a 90 percent grown monostratum against a porcine transmissible gastroenteritis virus, a porcine epidemic diarrhea virus and a porcine rotavirus respectively, and adding a cell maintenance media into the host-cell lines respectively to be cultured at 37 DEG C; after cytopathic effect reaches over 75 percent, collecting viruses to be stored at 20 DEG C below zero for standby; mixing the viruses according to 10 TCID50 in 1:1:1, and simultaneously adding Freund's complete adjuvant and immunopotentiator into the mixture to inactivate the mixture by formaldehyde at 37 DEG C for 24 hours; and adding an oil adjuvant into the mixture to prepare a vaccine of water-oil-water preparation. The method can be used for preparing the triple vaccine for preventing the porcine transmissible gastroenteritis, the porcine epidemic diarrhea and the porcine rotavirus so as to solve the problem that the diseases do not have an effective medicine to treat currently.

Description

technical field [0001] The invention relates to a triple vaccine of porcine transmissible gastroenteritis, porcine epidemic diarrhea and porcine rotavirus. Specifically, it is a triple inactivated vaccine prepared by cell culture and formaldehyde inactivation. Belongs to the field of bioengineering. Background technique [0002] Porcine transmissible gastroenteritis virus (TGEV) is a member of the Coronaviridae family and the genus Coronaviridae. TGEV virus can infect pigs of all ages, especially causing vomiting, severe diarrhea and high mortality (usually 100%) in piglets under the age of two weeks. , often occurs repeatedly and is popular in pig farms, and brings great harm to the pig industry (Pensaert M B. Disease of Swine. Seventh edition. Iowa State University Press. USA. 1992, 305-338). At present, there is no particularly effective means for the prevention and treatment of the disease. Viable treatment is symptomatic only, reducing starvation, dehydration, and a...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P1/12A61K39/225A61K39/12
Inventor 张桂红杨保收
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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