51results about How to "Strong refraction" patented technology

Two monoclonal antibodies B and C for the lymphocystics virus of bastard halibut are prepared from two hybrid tumor cells (CCTCC-C200419 and CCTCC-C200420) through conventional culture for 2-3 days to secrete a lot of said monoclonal antibodies B and C.

This invention discloses monoclonal antibody against anti-LCDV immunoglobulin of Paralichthys olivaceus, which is excreted by hybridoma JF-lgM-H (CCTCC-C200631). The method comprises: immunizing Paralichthys olivaceus with LCDV inactivated by formalin to prepare antiserum, purifying Paralichthys olivaceus immunoglobulin, immunizing Balb/c mice as antigen, preparing hybridoma cells by cell engineering method, and screening the monoclonal antibody by immunoassay. Indirect ELISA and indirect immunofluorescent antibody assay show that this monoclonal antibody is located on the heavy chain (7-80 kDa) of the anti-LCDV immunoglobulin. The monoclonal antibody can be used for preparing reagents for detecting LCDV infection in early stage, and evaluating the immune effects of LCDV vaccine inactivated by formalin.

The invention discloses a novel method for obtaining goat-induced multi-potent stem cells. The novel method comprises the following steps: firstly, extracting total RNAs from a testicular tissue, a skin tissue and a small intestine tissue of a goat; performing reverse transcription to obtain a cDNA segment; and with the cDNA segment as a template, cloning to obtain coding sequences of Oct4, Sox2, K1f4 and c-Myc multifunctional transcription factors of the goat. Fetal fibroblast cells of the goat and a mouse are obtained by utilizing a trypsin digestion method, feed-layer cells and a cell culture solution are prepared. mRNA of each multifunctional transcription factor obtained by utilizing an in-vitro transcription test is mediated by lipidosome 2000 to transfect the goat fetal fibroblast cells, so that goat iPS cells are prepared. A cell colony which is flatly cloned, dense, large in nuclear-cytoplasmic ratio, clear in cloning boundary and strong in light refraction is judged as a goat iPS cell colony. The goat iPS cell colony is picked in a monoclonal mode, digested by pancreatic enzyme and independently inoculated to a novel culture pore plate inoculated with a feeding layer to be further cultured to obtain a goat iPS cell line.

The invention discloses a monoclonal antibody capable of resisting a surface marker molecule CD4-2 of a T cell of paralichthys olivaceus. The monoclonal antibody is secreted by a hybridoma cell whichis named by hybridoma cell JF-CD4-2-1D8 and collected in the China Center for Type Culture Collection, with the collection number of CCTCC NO: C201880, in Wuhan University in Wuhan, Hubei, on March 29. 2018. An experimental result of indirect enzyme-linked immunosorbent assay of the monoclonal antibody shows that the monoclonal antibody can be specifically combined with CD4-2 polypeptide; an immunoblotting result shows that the monoclonal antibody disclosed by the invention can specifically identify the paralichthys olivaceus CD4-2 recombinant protein with the molecular weight of 25kDa and theparalichthys olivaceus natural CD4-2 protein with the molecular weight of about 33 kDa; indirect immunofluorence and flow cytometry show that the monoclonal antibody can specifically identify CD4-2 molecules on the surface of lymphocyte of the paralichthys olivaceus. As the monoclonal antibody capable of resisting the surface marker molecule CD4-2 of the T cell of the paralichthys olivaceus, an important tool is provided for detection of paralichthys olivaceus CD4-2+T lymphocyte; the monoclonal antibody has an important practical significance for deep understanding of immunologic mechanisms of fish cells and development of an efficient and environment-friendly fish vaccine.

The invention relates to a lead-free porcelain imitation glass wine bottle and a manufacture process thereof. The lead-free porcelain imitation glass wine bottle comprises the following materials in parts by weight: 45-50 parts of quartz sand, 10-12 parts of calcined soda, 10-11 parts of calcite, 5-8 parts of ultrafine talcum powder, 2-4 parts of magnesium chloride, 2-3 parts of clarifying agent, 4-6 parts of opacifying agent, 1-2 parts of curing agent and 1-2 parts of coupling agent. The lead-free porcelain imitation glass wine bottle provided by the invention is high-temperature resistant, white, safe, nontoxic, light-permeable, environment-friendly and energy-saving, has high refractivity, uniform texture and low cost, and can be recycled.

The invention discloses a monoclonal antibody for a paralichthys olivaceus resistant T cell surface marker molecule CD4-1. The monoclonal antibody is named as a hybridoma cell JF-CD4-1-2A10, the preservation number is CCTCC NO: C201879, the preservation organization is China Center For Type Culture Collection, the address is Wuhan university of Wuhan city in Hubei province, and the preservation date is March 29th, 2018, and is secreted by the hybridoma cell. An indirect enzyme-linked immunosorbent assay reaction experiment on the monoclonal antibody shows that the monoclonal antibody can be specially combined with paralichthys olivaceus CD4-1 polypeptide, indirect immune fluorescence and flow cytometry prove that the monoclonal antibody can specially identify the paralichthys olivaceus lymphatic cell surface CD4-1 molecule, and an immunoblotting result shows that the monoclonal antibody disclosed by the invention can specially identify paralichthys olivaceus CD4-1 recombinant protein and natural paralichthys olivaceus CD4-1 protein of which the molecular weight is 52 kDa. According to the monoclonal antibody disclosed by the invention, the monoclonal antibody for the paralichthys olivaceus resistant T cell surface marker molecule CD4-1 is prepared, and an important tool is provided for detecting paralichthys olivaceus CD4-1<+T> lymphatic cells.

The invention discloses an anti-paralichthys olivaceus immunoglobulin D monoclonal antibody. The anti-paralichthys olivaceus immunoglobulin D monoclonal antibody is secreted by hybridoma cells of a hybridoma cell strain JF-IgD, with the collection No.: CCTCCNO: C2014159, the collection unit: China Center for Type Culture Collection, the collection address: Wuhan University in Wuhan, Hubei of China, and the collection date: September 2, 2014. Indirect enzyme-linked immune reaction experiment results show that the anti-paralichthys olivaceus immunoglobulin D monoclonal antibody can be specifically bound with recombinant flounder IgD heavy chain proteins; immunoblotting results show that the anti-paralichthys olivaceus immunoglobulin D monoclonal antibody can specifically recognize recombinant protein encoded by flounder IgD heavy chain genes delta1-delta 4 and flounder natural IgD heavy chain proteins with the molecular weight of 120kDa. The anti-paralichthys olivaceus IgD monoclonal antibody is prepared, and an important tool is provided for detecting flounder IgD protein molecules and IgD surface positive (IgD +) lymphocytes.

The invention discloses an ultra-wideband low-RCS plasma antenna based on a reconstruction structure, and the antenna comprises a high-frequency band antenna, an on-off switch, and a plasma antenna which is located above the high-frequency band antenna, has a working frequency band of an adjustable and controllable antenna, and has a low-RCS characteristic. The on-off switch is connected between the high-frequency band antenna and the plasma antenna. The plasma antenna comprises a chassis and a plasma grid array, wherein the plasma grid array is assembled above the chassis, and the plasma grid array is electrically connected with the high-frequency band antenna through the on-off switch. The antenna has the advantages of good invisibility, high dynamic adjustability and low mutual coupling effect.

The invention discloses a simple, highly-efficient and mechanized inducing method for differentiation of human pluripotent stem cells into retinal tissues. The inducing method comprises the followingsteps: digesting hPSCs so as to obtain cell pellets, then culturing the cell pellets so as to obtain embryoid bodies, and inducing the embryoid bodies for differentiation so as to obtain 3D retinal tissues. The inducing method is characterized in that the hPSCs are digested to obtain the cell pellets which are cultured to obtain the embryoid bodies, that is, the hPSCs are digested into single cells which are collected to be added into a culture container for cell reaggregation to form the embryoid bodies. The inducing method provided by the invention can enable induced differentiation and acquisition of the retinal tissues to realize pipeline operation, and can realize industrialized production for application in large-scale clinical application or drug screening.

The invention relates to the field of medical apparatus and instruments, and especially relates to high-intensity focused ultrasound-puncture ablation fusion therapy equipment. The therapy equipment includes a focused ultrasound transmitter capable of emitting focused ultrasound; and an image guiding device for displaying the focus and surrounding body tissue of patients and a set of puncture ablation assemblies are integrally arranged on the focused ultrasound transmitter. Thus, the defects in the prior art that are hardly overcome during the independent using of focused ultrasound and radiofrequency ablation can be overcome; through the fusion of focused ultrasound and radiofrequency ablation, mutual complement functions can be achieved, so that treatment quality can be guaranteed whiletreatment time is shortened; the therapy equipment is accurate and conformal, and can perfectly protect normal tissue and expand treatment indications while thorough treatment is guaranteed; and a novel method of thorough, fast, safe and minimally invasive treatment can be provided for tumor patients of which diseases are hard to treat or even cannot be treated.

The present invention relates to an anti-UV flash fabric including a lower cotton cloth layer (1), a wool layer (2) and an upper cotton cloth layer (3), a base layer (6) is compounded under the lower cotton cloth layer (1), the surface of the upper cotton cloth layer (3) is covered with an anti-static layer (4), the anti-static layer (4) is made from core-spun yarn fiber, the core-spun yarn fiber comprises a yarn (4.1) and a metal wire (4.2), and the surface of the anti-static layer (4) is provided with an anti-UV layer (5). The base layer is made of a fabric, the fabric is interwoven by warp yarns and weft yarns, and cross-sections of the warp yarns and the weft yarns are triangular. The anti-UV flash fabric can be ultraviolet-proof, has anti-static and anti-radiation functions, and can flash.

The invention discloses a pleuromutilin derivative containing a 1, 2, 4-triazole acrylamide side chain as well as a preparation method and application of the pleuromutilin derivative, and belongs to the field of medical chemistry. According to the compound, pleuromutilin and different substituted acrylic acid are used as raw materials, 3-sulfydryl-1, 2, 4-triazole is used as a connector under the action of a catalyst, and the pleuromutilin derivative which is not reported, has the activity of resisting drug-resistant bacteria and contains a 1, 2, 4-triazole acrylamide side chain is synthesized. The synthesis method is high in operation safety, mild in reaction condition and suitable for industrial production. Preliminary biological activity tests and safety evaluation show that the pleuromutilin derivative containing the 1, 2, 4-triazole acrylamide side chain and having the drug-resistant bacterium resisting activity has good drug-resistant bacterium resisting activity and safety, so that the pleuromutilin derivative can be applied to treatment of infectious diseases, especially infectious diseases caused by drug-resistant bacteria, and has good application prospects. The medicine development value is very good.

The invention discloses a cage inlaid intelligent jewel which comprises a base, wherein the base is internally provided with a label chip; the base is provided with a metal ring; the two ends of the metal ring are connected by a metal support group; the label chip is connected with the metal ring through a lead wire; a first jewel monomer is arranged in the middle of the upper end face of the base; four second jewel monomers are arranged at the circumference of the first jewel monomer and are circular; third jewel monomers are arranged in splicing seams formed between any two adjacent second jewel monomers; fourth jewel monomers are further arranged between any two adjacent second jewel monomers; the fourth jewel monomers are inlaid between the second jewel monomers in a pressing manner; an outer ring of the base is further sleeved with a metal cage; an opening is formed at the upper part of the metal cage; and the diameter of the opening is greater than the maximum outside diameter ofan improved combination jewel. The label chip is inlaid into the jewel; various information of the jewel can be stored in the label chip; and long-term storage of the information is facilitated.

The invention relates to a shiny fabric cashmere sweater convenient to wear. The shiny fabric cashmere sweater convenient to wear comprises a sweater body (1), wherein sleeves of the sweater body are made of a fabric; a left collar body and a right collar body which form a collar (2) of the sweater body (1) are connected through a buckle; the sweater body (1) is further provided with pockets (6); thickening strips are arranged at the collar (2) and top flies (3) of the sweater body (1); strip-type protrusions are vertically arranged on a lower hem (4) of the sweater body (1); decorative buttons (5) are arranged on one top fly (3) of the sweater body (1); the fabric is formed by interweaving warp yarns and weft yarns, and the cross sections of the warp yarns and the cross sections of the weft yarns are all triangular. The cashmere sweater has the advantage of being convenient to wear. The adopted fabric is shiny.

The invention discloses a holographic anti-blue-ray thin film and a making method thereof. The method comprises the following steps of S10, selecting a recording medium and recording light source of optical responses, and making a volume hologram; S20, calculating a Bragg reflection angle according to Bragg conditions and reproduction characteristics of the volume hologram; S30, making the reflective holographic anti-blue-ray thin film according to the Bragg reflection angle obtained in S20. The holographic anti-blue-ray thin film and the making method thereof have the advantages that the holographic anti-blue-ray thin film is simple to make and low in making cost, harmful short-wave blue rays can be effectively reflected, and meanwhile, the situation is avoided that the color of an object is distorted because beneficial long-wave blue rays are reflected, so that the holographic anti-blue-ray thin film helps people to use their eyes healthily.

The invention relates to an elegant pocketed women's cashmere sweater made of flickering fabric. The elegant pocketed women's cashmere sweater comprises a sweater body (1), the sweater piece of the sweater body is made of cashmere, sleeves are made of fabric, a thickening strip is vertically arranged in the front middle of the sweater body (1), decorative buttons (4) are arranged on the thickening strip, a pocket (5) is arranged on the sweater body (1), a thickening strip is arranged at the position of a collar (2) of the sweater body (1), annular thickening strips are arranged at the positions of sleeve openings of the sweater body (1), and stripped protrusions are vertically arranged at a lower hem (3) of the sweater body (1); the fabric includes woven fabric and knitted fabric, and the woven fabric and the knitted fabric are bonded together through binding agents. The elegant pocketed women's cashmere sweater is elegant, and the flickering fabric is adopted.

The invention relates to a cashmere sweater high in wearing layering and glossiness. The cashmere sweater comprises a clothes body (1), and the front and sleeves of the clothes body are made of cashmere. A collar of the clothes body (1) is a V-shaped collar (2) and is provided with a connecting piece (4) matched with the V-shaped collar (2), the edge of the connecting piece (4) is sewn to the position of the edge of the V-shaped collar (2), the connecting piece (4) is provided with a round collar (5), and the lower end of the collar of the V-shaped collar (2) extends to the position close to a lower hem (3) to form a deep V-shaped collar structure. The back of the clothes body is made of a fabric, the fabric is formed by interweaving warp yarn and weft yarn, and the cross sections of the both the warp yarn and the weft yarn are triangular. The cashmere sweater has the advantage of being high in layering after being worn. The fabric is high in glossiness.