51results about How to "Strong refraction" patented technology

The present invention has an object to solve the problem in viewing angle characteristics. A display device of the present invention includes: a light shielding film (34) including a plurality of fixed apertures (36), each allowing passage of light; a lens (44) arranged above the plurality of fixed apertures (36); a plurality of shutters (14), which are arranged between the light shielding film (34) and the lens (44) and correspond to the plurality of fixed apertures (36), respectively, for controlling passage and blocking of the light; and a drive portion (40) for driving each of the plurality of shutters (14) to move between a position above each of the plurality of fixed apertures (36) and a position retracted from each of the plurality of fixed apertures (36). The plurality of fixed apertures (36) each have an elongated shape including a long-side direction (Y) and a short-side direction (X). The lens (44) has a light refractive power that is larger in the short-side direction (X) than in the long-side direction (Y).

Two monoclonal antibodies B and C for the lymphocystics virus of bastard halibut are prepared from two hybrid tumor cells (CCTCC-C200419 and CCTCC-C200420) through conventional culture for 2-3 days to secrete a lot of said monoclonal antibodies B and C.

The invention is the monoclonal antibody of flounder against lymphocyst virus (LCDV) immunoglobulin. It is secreted by the hybridoma cell named as hybridoma cell JF-IgM-H, and the preservation number is CCTCC-C200631. The preparation method of the monoclonal antibody is as follows: prepare antiserum by immunizing flounder with formalin-inactivated LCDV, then purify the flounder immunoglobulin as an antigen and immunize Balb / c mice, and use cell engineering to generate fusion hybrid Tumor cells, the monoclonal antibody was screened out by immunological detection method. Indirect enzyme-linked immunosorbent method and indirect immunofluorescence antibody method comprehensively determine: the specific binding reaction between the monoclonal antibody of the present invention and the flounder anti-LCDV immunoglobulin really exists; transfer immunoblotting method determines: the epitope of the monoclonal antibody of the present invention is located at the molecular weight On the heavy chain of the flounder anti-LCDV immunoglobulin in the range 70-80 kDa. The invention will be used in the preparation of reagents for early detection and judgment of LCDV infection in flounder and evaluation of the immune effect of LCDV formalin inactivated vaccine on flounder.

The invention discloses a method for cultivating primary hippocampal neurons. The method comprises: (1) digesting the hippocampal tissue; (2) blowing and dispersing the digested hippocampal tissue to obtain a cell suspension; (3) The cell suspension is inoculated and first cultivated for 2-4 hours to make the cells adhere to the wall; (4) the first culture solution in the culture dish is replaced with the second culture solution, and the second culture is carried out for 24 hours; (5) to Add 1-2 μg / ml of cytarabine to the culture dish, and carry out the third culture for 24 hours; (6) Use the second culture medium to replace half of the medium of the culture dish, and carry out the fourth culture for at least 3 days, during which every The medium was changed twice a week to obtain primary hippocampal neurons. The method can be used to quickly and efficiently prepare primary hippocampal neurons, and the obtained primary hippocampal neurons are extremely suitable for use as cell models for patch clamp experiments.