Monoclonal antibody for paralichthys olivaceus resistant T cell surface marker molecule CD4-1 as well as preparation method and application thereof

A monoclonal antibody, cell surface technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, microorganism-based methods, biochemical equipment and methods, etc., to achieve novel design effects
CN108341875AActive Publication Date: 2018-07-31OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2018-07-31

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Abstract

The invention discloses a monoclonal antibody for a paralichthys olivaceus resistant T cell surface marker molecule CD4-1. The monoclonal antibody is named as a hybridoma cell JF-CD4-1-2A10, the preservation number is CCTCC NO: C201879, the preservation organization is China Center For Type Culture Collection, the address is Wuhan university of Wuhan city in Hubei province, and the preservation date is March 29th, 2018, and is secreted by the hybridoma cell. An indirect enzyme-linked immunosorbent assay reaction experiment on the monoclonal antibody shows that the monoclonal antibody can be specially combined with paralichthys olivaceus CD4-1 polypeptide, indirect immune fluorescence and flow cytometry prove that the monoclonal antibody can specially identify the paralichthys olivaceus lymphatic cell surface CD4-1 molecule, and an immunoblotting result shows that the monoclonal antibody disclosed by the invention can specially identify paralichthys olivaceus CD4-1 recombinant protein and natural paralichthys olivaceus CD4-1 protein of which the molecular weight is 52 kDa. According to the monoclonal antibody disclosed by the invention, the monoclonal antibody for the paralichthys olivaceus resistant T cell surface marker molecule CD4-1 is prepared, and an important tool is provided for detecting paralichthys olivaceus CD4-1<+T> lymphatic cells.
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Description

technical field

[0001] The invention relates to a monoclonal antibody and a preparation method thereof, in particular to an anti-flounder ( Paralichthys olivaceus ) The monoclonal antibody of T cell surface marker molecule CD4-1 and the preparation method thereof belong to the technical field of fish molecular immunology. Background technique

[0002] CD4 molecule is a single-chain transmembrane glycoprotein, which is a member of the Ig superfamily and is mainly expressed on the surface of mature T lymphocytes. CD4 + Cells, as an important class of T lymphocytes, participate in humoral immunity and cellular immunity, and play a vital role in resisting pathogen invasion and other related immune defenses. CD4 + T cell TCR recognizes exogenous antigenic peptide-MHC on the surface of APC After the molecule-like complex is activated, Th cells produce a variety of cytokines, chemotaxis and activate other immune cells to clear pathogens. The CD4 molecule is also a receptor...

Claims

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