The invention discloses a kit for preparing human body dopaminergic neurons and application thereof, and belongs to the field of biopharmacys. The kit comprises a fibroblast efficient separation culture solution, chemical modification mRNA, an induction culture solution, a small molecule compound induction combination 1 and an induction combination 2 for promoting maturation and survival. The invention also provides an application of the kit in reprogramming the skin fibroblasts of the Parkinson's patient to generate the dopaminergic neurons. According to the kit, the combination of cmRNA-ASLC1, cmRNA-LMX1A and cmRNA-NURR1 is generated by using the chemically modified mRNA (cmRNA) so as to directly reprogram the human fibroblasts into the dopamine nerve cells, the inherent risk of genome integration and insertion mutagenesis in the DNA method is eliminated by using the chemically modified mRNA (cmRNA), the transgenic tracer agent is not left, the small molecule combination is used to optimize the induction culture system. The generation efficiency and the maturity of the dopaminergic neurons are improved.