The invention discloses (S)-1,3-thiazolyl phenyl furan thioformate compounds and a preparation method and application thereof. The compounds have a strong inhibitory effect on a hpa1 gene promoter ina pathogen T3SS system, and the inhibition rate of some compounds exceeds 97%, and the compounds can be used as a T3SS system inhibitor; some of the compounds strongly inhibit the plant pathogen T3SSsystem, does not affect the growth of plant pathogenic bacteria meanwhile, but only significantly reduces the pathogenicity of the plant pathogenic bacteria to achieves the prevention and/or treatmentof plant diseases caused by the plant pathogenic bacteria; the compounds can be used as a plant pathogen type III secretion system inhibitor, or used for preparing a drug for related the plant diseases for preventing and/or treating the plant diseases, also have the effect of reducing and delaying the resistance of the plant pathogenic bacteria to the compounds, and have a longer effective life for prevention and/or treatment of the plant diseases and broad application prospects..