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Tumor vaccine using salmonella as carrier and preparation method thereof

A technology for Salmonella and tumor vaccines, applied in the field of tumor vaccines and their preparation, can solve problems such as the length limitation of fusion antigens, and achieve the effects of large-scale production, large antigen expression, and simple use

Inactive Publication Date: 2008-06-25
BEIJING NACK CHANGJIN INT ARGENTUM ION TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is that the length of the fusion antigen has certain limitations

Method used

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  • Tumor vaccine using salmonella as carrier and preparation method thereof
  • Tumor vaccine using salmonella as carrier and preparation method thereof
  • Tumor vaccine using salmonella as carrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the preparation of tumor vaccine and its effect

[0056] 1. Preparation of tumor vaccines

[0057] The tumor vaccine is recombinant attenuated Salmonella typhimurium SL3261 / pNSspH-HBx, and the specific preparation method is as follows:

[0058] 1. Materials

[0059] (1) Bacteria and cell culture:

[0060] Salmonella typhimurium SL3261 has been widely used in vaccine construction (for example (Ugrinovic S, BrooksCG, Robson J, Blacklaws BA, Hormaeche CE, Robinson JH: H2-M3 majorhistocompatibility complex class Ib-restricted CD8 T cells induced by Salmonella enterica serovar Typhimurium infection recog proteins released by Salmonella serovar Typhimurium. Infect Immun 2005, 73(12):8002-8008), and available from the Salmonella Genetic Stock Center (www.ucalgary.ca / ~kesander)). Bacteria were cultured in nutrient broth or Luria Bertani broth (see literature for detailed composition (Liu SL, Sanderson KE: A physical map of the Salmonellatyphimurium LT2 genome m...

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Abstract

The invention discloses a tumor vaccine using attenuated salmonella as a vector and a preparation method thereof.Active component of the tumor vaccine is recombinant attenuated salmonella, the construction method of the recombinant attenuated salmonella includes the following steps: 1) an expression vector for expressing recombinant prokaryotic cell of confusion protein is constructed; the confusion protein comprises a N side signal area (namely a N side guiding secretion area[1]) of effect proteins(SPI-2 TTSS) in a III-type secretion system of salmonella and at least a protein antigen or a protein epitope of the tumor; the expression vector of the recombinant prokaryotic cell is obtained by introducing encoding gene of the confusion protein into multiple cloning sites of the expression vector of the prokaryotic cell; 2) the expression vector of the recombinant prokaryotic cell is introduced to the attenuated salmonella to obtain the recombinant attenuated salmonella expressing the confusion protein. The tumor vaccine in the invention can be used for preventing the tumor.

Description

technical field [0001] The invention relates to a tumor vaccine with attenuated Salmonella as a carrier and a preparation method thereof. Background technique [0002] Effective tumor vaccines have been the goal of scientists all over the world for many years. However, humans have not been successful so far. The main reason is that the immunogenicity of tumor-specific antigens is often very weak, and it is difficult to stimulate the body to produce a sufficient anti-tumor immune response. Therefore, how to fully express the immunogenicity of tumor cells has become the most prominent challenge in this research field. [0003] The role of tumor vaccines is to use specific and immunogenic tumor antigens to activate, restore or strengthen the body's anti-tumor immune response and eliminate residual and metastatic tumor cells. At present, tumor vaccines have been developed to the second generation. The first-generation vaccine is made by adding a non-specific adjuvant to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/112A61P35/00C12N1/21C12N15/62C12N15/74
CPCY02A50/30
Inventor 库宝善李承臻刘树林
Owner BEIJING NACK CHANGJIN INT ARGENTUM ION TECH DEV
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