65 results about "Xanthomonas oryzae" patented technology

The invention discloses a corn NAC transcription factor and application thereof in enhancing disease resistance. The corn NAC transcription factor can endow transgenic rice with wide resistance and does not affect the rice yield, and the amino acid sequence of the corn NAC transcription factor is SEQ ID No.2. The sequence of the coding gene of the gene is SEQ ID No.1. Research shows that the corn NAC transcription factor is located in a cell nucleus, the resistance of rice to rice blast caused by magnaporthe oryzae and rice bacterial leaf blight caused by Xanthomonas oryzae can be improved by overexpression of the corn NAC transcription factor in rice, meanwhile, the agronomic traits of the rice are evaluated, it is found that the gene does not affect the ear length and the grain length of the rice, and the corn NAC transcription factor can be used for improving the rice resistance to rice blast caused by magnaporthe oryzae and the rice bacterial leaf blight caused by Xanthomonas oryzae. Thousand seed weight and single plant yield.

The invention belongs to the technical field of biological prevention and control, and particularly relates to a Burkholderia paragallinarum D15 and application of the Burkholderia paragallinarum D15. The Burkholderia parakholderia sp. D15 is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 25, 2024, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 1.61962. The Burkholderia parakholderia sp. D15 has the advantages that the Burkholderia parakholderia sp. According to the invention, a multifunctional strain Burkholderia paragallinarum D15 is separated from the rock wall of Danxia red rock, and the Burkholderia paragallinarum D15 has a relatively good antagonistic effect on most plant pathogens and has a relatively strong bacteriostatic effect on xanthomonas oryzae Xoo causing rice bacterial blight; the compound also has a relatively strong bacteriostatic effect on other pathogenic bacteria causing crop diseases, such as rice sheath blight disease, rice blast, fusarium graminearum, sclerotium rolfsii and ralstonia solanacearum, and lays a foundation for prevention and treatment of rice bacterial blight and development of related biological preparations.

The invention relates to an application of carboxylated chitin or carboxylated chitosan in promoting insect-killing fungi to prevent and treat diseases and insect pests. Research finds that carboxylated chitin or carboxylated chitosan can significantly promote spore germination and hypha growth of insecticidal fungi, also can significantly enhance stress resistance of the insecticidal fungi, significantly enhance toxicity of the insecticidal fungi to pests, improve ultraviolet resistance and high temperature resistance of the insecticidal fungi, and improve the insecticidal activity of the insecticidal fungi. The invention also finds that the carboxylated chitin or chitosan can effectively inhibit the growth of Xanthomonas oryzae pv. Oryzae. Therefore, the carboxylated chitin or the carboxylated chitosan can be used for preparing a product for enhancing the insecticidal fungus effect and a product for preventing and treating the rice bacterial leaf blight, and the prevention and treatment of plant diseases and insect pests are realized. In addition, carboxylated chitin and carboxylated chitosan are derived from natural polymer materials, have the characteristics of safety, no toxicity, biodegradability, environmental friendliness and the like, are environment-friendly and economical, and have wide application prospects and market values.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

As a main grain crop in the world, the yield and quality of rice are often threatened by bacterial leaf blight and rice blast. According to the invention, a recombinant expression vector for expressing STTM-miR169d / p is constructed by starting from regulation and control of the expression quantity of miRNA through an STTM technology. By adopting an agrobacterium tumefaciens-mediated rice protoplast transformation technology, a rice plant containing the STTM-miR169d / p transgenic fragment is obtained. Through field inoculation of xanthomonas oryzae pv. Oryzae and inoculation of rice blast spores on in-vitro leaves, it is verified that the STTM-miR169d / p transgenic rice has significantly improved resistance to xanthomonas oryzae pv. Oryzae and rice blast compared with wild type rice. The invention not only provides a new germplasm resource for cultivating disease-resistant rice, but also provides a new thought for regulating and controlling the disease resistance of rice from the aspect of miRNA.

The present invention discloses the application of isoquinoline-3-carboxylic acid in preventing and controlling plant bacterial diseases. The isoquinoline-3-carboxylic acid provided by the present invention has an obvious inhibitory effect on plant bacterial diseases, and the plant bacterial diseases are Ralstonia solanacearum, Acidovorax avenae subsp. Citrulli, Pseudomonas syringae pv. actinidiae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Alternaria brassicae, Xanthomonas citri subsp. citri, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas fragariae, Pseudomonas syringae pv. lachrymans, Dickeya dadantii, Xanthomonas axonopodis pv. mangiferaeindicae. The isoquinoline-3-carboxylic acid of the present invention has protective and therapeutic effects on Acidovorax avenae subsp. Citrulli.

The invention discloses leuconostoc mesenteroides a3 antagonizing xanthomonas oryzae and application of the leuconostoc mesenteroides a3, and belongs to the technical field of microorganisms. The leuconostoc mesenteroides a3 is preserved in the China Center for Type Culture Collection on April 7, 2025, the preservation number of the strain is CCTCC No: M 2025646, and the leuconostoc mesenteroides a3 has high antagonistic activity on rice bacterial leaf blight germs and rice bacterial leaf streak germs. The biocontrol microbial inoculum prepared from the strain has a good biocontrol effect on rice bacterial leaf blight and rice bacterial leaf streak, the use of chemical pesticides can be reduced, the safety of grains and the ecological environment can be improved, and good economic and social benefits can be brought.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method and application of five compounds (1-5) extracted from liquid fermentation of Chaetomium sp. Specifically, the present application discloses five compounds, wherein compound 1 is a new compound. The Chaetomium sp. metabolite has good antibacterial activity on three tested pathogenic bacteria (Pseudomonas syringae pv. actinidiae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola), and has important significance for development of a new type of microbial source antibacterial agent.

The invention discloses a rapid detection method for Xanthomonas oryzae pv. Oryzae on the basis of RPA-CRISPR / Cas12b. The reagent combination comprises a specific RPA primer pair and sgRNA, and the sequences of the specific RPA primer pair and the sgRNA are respectively shown as SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The detection method comprises the following steps: taking nucleic acid of a sample to be detected as a template, and performing RPA isothermal amplification by utilizing the primer pair; the amplification product is mixed with sgRNA, Cas12b protein and fluorescent reporter molecules for incubation, and the trans-cleavage activity of Cas12b is activated; a result is judged by detecting a fluorescence signal. According to the invention, the optimized RPA and CRISPR systems are integrated on the centrifugal micro-fluidic chip, so that the full-flow closed tube and automatic detection is realized, the detection can be completed within 30 minutes, the sensitivity reaches 0.156 pg / reaction, no non-specific reaction is caused to five related bacteria such as Xa and Xap, the sensitivity to XOO3 subtype is high, but the kit is not suitable for XOO2 subtype, and the kit has the advantages of rapidness, sensitivity, specificity and low equipment dependence degree, and can be used for detecting the related bacteria of XOO3 subtype and XOO2 subtype. The method is suitable for non-diagnostic detection of field environments, port environments and the like.

The invention discloses a composite nano preparation based on zinc sulfide quantum dots as well as a preparation method and application thereof, and relates to the technical field of nano agriculture and plant disease prevention and control. The nano preparation disclosed by the invention has high antibacterial efficiency, and the prepared ZnSQDs and the composite nano preparation of the ZnSQDs can show a remarkable inhibition effect on the Xanthomonas oryzae pv.oryzae (Xoo) under the condition of an extremely low dosage (2 to 10 mg / L), so that the ZnSQDs and the composite nano preparation of the ZnSQDs can be used for inhibiting the Xanthomonas oryzae pv.oryzae (Xoo) of the Xanthomonas oryzae pv.oryzae (Xoo); compared with the traditional chemical pesticide which can achieve the prevention and treatment effect only through the dosage of 100 milligrams per liter or even gram, the effective dosage of the compound is greatly reduced, and the compound shows ultrahigh antibacterial activity; meanwhile, due to the high specific surface area and active surface sites of the nano-material, the nano-material can be in full contact with pathogenic bacteria, and the action efficiency is improved, so that breeding and diffusion of the pathogenic bacteria can be effectively inhibited in the early stage, and diseases are remarkably reduced.

The invention belongs to the technical field of plant genetic engineering, and relates to an application of a Xanthomonas oryzae hexose phosphate mutase encoding gene in the aspect of adjusting the susceptibility of cruciferous plant arabidopsis thaliana. According to the present invention, the recombinant plasmid is constructed, the Xanthomonas oryzae pv.oryzae (Xoo) PXO99A hexose phosphate mutase coding gene PXO03174 is adopted as the target gene to be introduced into the cruciferous plant arabidopsis thaliana, such that the condition that the plant with the overexpression of the gene is sensitive to the infection of the pathogenic bacterium Pseudomonas syringae pv.tomato (Pst) DC3000 is found, and the significantly enhanced disease infection characteristic is represented; according to the present invention, the discovery can be used for developing the sensitive bacterial disease detection method so as to improve the accuracy of bacterial disease detection; the method can also be used for identifying toxic genes of plant pathogenic bacteria such as pathogenic bacteria sensitive plants and pseudomonas syringae and researching the mechanism of the infected plants, and can also be used for researching the disease resistance of the plants to the pseudomonas syringae and developing antibacterial agents.

The invention discloses a purine nucleoside compound containing a cinnamic acid structure as well as a preparation method and application of the purine nucleoside compound, and a series of purine nucleoside compounds containing the cinnamic acid structure are synthesized by taking a purine nucleoside compound as a basis and introducing a piperazine-containing cinnamic acid fragment into a system. The compound has a good inhibition effect on plant pathogenic microorganisms, and especially has a good inhibition effect on plant pathogenic viruses such as tobacco mosaic viruses. Wherein the activity data of a part of the compounds is superior to that of a commercial drug ribavirin, the activity of the compounds is equivalent to that of ningnanmycin, particularly, the compound 14 has excellent treatment activity, and the EC50 of the compound 14 is 274.25 mu g / mL. The compound has good antibacterial activity on Xanthomonas oryzae pv. Oryzae, when the concentration is 50 [mu] g / mL, the inhibitory activities of the compound 7, the compound 20 and the compound 21 on Xanthomonas oryzae pv. Oryzae are 36.2%, 40.3% and 35.6% respectively and are superior to 32.6% of a control agent thiediazole copper, when the concentration is 50 [mu] g / mL or below, the inhibitory effects of the compound 1, the compound 3, the compound 23 and the compound 24 on Xanthomonas citri are 21.3%, 20.4%, 27.8% and 22.6% respectively, and the compound 7, the compound 20 and the compound 21 on Xanthomonas oryzae pv. Oryzae are superior to that of the control agent thiediazole copper. And the results are better than that of a control agent thiediazole copper by 18.6%.

The application discloses application of a carbazole derivative in prevention and treatment of plant diseases and relates to the technical field of medicinal chemistry. The structure of the carbazole derivative is as follows: The carbazole derivative has excellent inhibiting effect on plant pathogenic bacteria such as Xanthomonas oryzae ACCC 11602, Xanthomonas axonopodis pv.Citri, Pseudomonas soli maceraum and Erwinia aroideae, and has the advantages of simple structure, cheap raw materials and easy synthesis, and has the value of further research and development.

The invention discloses an alkyl-substituted BCP phosphinate compound and a synthesis method and application thereof.According to the method, [1.1. 1] propeller alkane, Katritzky pyridinium salt and a trivalent organic phosphine compound serve as raw materials, bis [2-(2, 4-difluorophenyl)-5-trifluoromethylpyridine] [2-2 '-dipyridyl] iridium bis (hexafluorophosphonic acid) salt serves as a catalyst, and the alkyl-substituted BCP phosphinate compound is synthesized through a one-pot method. And a series of alkyl substituted BCP phosphinate compounds are simply, conveniently and efficiently synthesized by photocatalysis of free radical bifunctionalization reaction of [1.1. 1] propeller alkane. The synthesis method disclosed by the invention has the characteristics of simple and easily available raw materials, mild and efficient reaction and the like, and the synthesized alkyl substituted BCP phosphinate compound has bactericidal activity on rice plant pathogen xanthomonas oryzae pv. Oryzae Xoo and is expected to be used for creating green pesticides.

The present invention discloses a rice SnRK1β subunit protein related to plant disease resistance, its coding gene and applications. The SnRK1β protein provided by the present invention is the protein shown in Sequence 2, 4 or 6, and the coding gene of the SnRK1β subunit protein is the DNA molecule shown in Sequence 1, 3 or 5. The knockout of the SnRK1β subunit enhances the resistance to rice blast and bacterial blight. After treatment with pathogen-associated molecular pattern (Chitin), it leads to the up-regulated expression of pathogenesis-related proteins, the accumulation of ROS levels, and the activation of the MAPK pathway. In addition, the deletion of the SnRK1β1A gene does not affect the main agronomic traits of rice, indicating that the SnRK1β gene has good application potential in enhancing crop disease resistance. The present invention uses genetic engineering means to regulate the expression level of the SnRK1β gene for molecular breeding, which has potential application value in improving crop disease resistance.

The invention relates to pseudomonas in northwest province and application thereof in prevention and treatment of bacterial blight of rice, the strain is preserved in China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.25493, the preservation date is August 5, 2022, and the preservation number is CGMCC NO.25493. The strain can be used for preventing and treating bacterial leaf blight caused by xanthomonas oryzae pv. Oryzae pv. Oryzae, the effect is remarkable, the prevention effect in a pot experiment reaches 72%, meanwhile, the disease time can be delayed, and therefore the strain has the huge potential of being applied as a biopesticide.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

The application belongs to the technical field of genetic transformation, and particularly relates to application of a lyase XooRuvC screened from a genome of Xanthomonas oryzae in improving resistance of rice to bacterial leaf blight. In the embodiment of the application, overexpression of the lyase XooRuvC in rice can significantly improve the resistance of rice to bacterial leaf blight without affecting normal growth and breeding of the crop, and is beneficial to cultivation of resistant varieties.

This invention discloses the promoter of the rice Os04g0650800 gene and its applications, belonging to the field of plant biotechnology. By cloning the promoter sequence of the Os04g0650800 gene, a recombinant expression vector pPGDH-YFP driving the yellow fluorescent protein gene was successfully constructed. Positive rice plants were screened by infecting Nipponbare rice with Agrobacterium. The T0 generation of positive rice seedlings were inoculated with Xanthomonas oryzae pv. oryzae. Xoo Using a confocal microscope, it was discovered that... Xoo Infection of positive rice plants can induce YFP expression. Genetic engineering of rice using this promoter, such as regulating the efficient expression of target genes through this promoter, will yield positive results. Xoo Infected rice plants; if this promoter is used to drive the expression of disease resistance-related genes in rice, rice plants resistant to bacterial blight will be obtained.

The structural formula of the thymol-containing quaternary ammonium salt derivative is shown as (1), wherein R1 is 2-isopropyl-5-methylphenyl, ethyl or a benzene ring, R2 is 2-chloro-5-methylthiophene, 3-methyl-2-methoxycarbonyl furan, 4-methylnaphthalene and differently substituted benzyl, X-is Cl-and Br-, and n is equal to 3, 5, 7, 8 and 9. The preparation method of the drug-loaded antibacterial liposome comprises the step of jointly assembling hydrogenated soybean phosphatidylcholine, cholesterol hemisuccinic acid monoester, phosphatidyl ethanolamine and a target compound thymol-containing quaternary ammonium salt derivative into the drug-loaded antibacterial liposome. Part of the compounds have excellent antibacterial activity on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri and Xanthomonas syringae pv. Kiwifruit. Meanwhile, the compound also has excellent biological activity on six fungi such as fusarium oxysporum and TMV viruses. A field test on Xoo shows that the protection effect of the drug-loaded antibacterial liposome is improved by 1.94 times, the treatment effect is improved by 1.56 times, and the drug-loaded antibacterial liposome shows remarkable pH-triggered release characteristics and colloidal stability. (1)

The invention belongs to the technical field of plant genetic engineering, and discloses a gene OSD4 participating in a synthetic route of PAL-dependent salicylic acid (SA) of rice and application of the gene OSD4. A mutant osd4 is obtained by knocking out OSD4 through CRISPR / Cas9, the SA content of leaves of the mutant osd4 is reduced to 15% of that of a wild type, and the mutant osd4 is more sensitive to xanthomonas oryzae pv. And constructing an OSD4 overexpression vector to obtain a transgenic material with enhanced resistance. Hormone determination and inoculation experiments show that OSD4 regulates SA synthesis and participates in disease-resistant reaction. The in-vitro enzyme activity is verified by constructing an OSD4-pMAL carrier, and it is proved that OSD4 can catalyze BS to directly generate SA. The gene can be used for disease resistance improvement of crops such as rice.

The invention discloses application of a SbHDA3 gene in regulation and control of plant disease resistance, and belongs to the technical field of gene engineering. The nucleotide sequence of the SbHDA3 gene is as shown in SEQ ID NO. 11. It is found that the SbHDA3 gene is remarkably related to plant disease resistance, the resistance of plants to xanthomonas oryzae, magnaporthe oryzae and colletotrichum gloeosporioides can be improved by overexpressing the gene, and the resistance is reduced if the gene is silent. The research result of the invention provides a new gene resource for plant disease-resistant breeding, and lays a theoretical foundation for researching a plant disease-resistant mechanism.

The invention provides a pleuromutilin derivative containing a thioacetate side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I

The invention provides a pleuromutilin derivative containing an amino side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The invention discloses a salt-tolerant nitrogen-fixing Klebsiella pasteurii and application thereof, and relates to the technical field of microorganisms, and the Klebsiella pasteurii S8812 provided by the invention is a salt-tolerant combined nitrogen-fixing bacterial strain which is screened from corn roots and has outstanding comprehensive performance; the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 21, 2025, and the preservation number of the strain is CGMCC No: 33923. The strain is high in nitrogen fixation activity, strong in salt resistance and capable of tolerating a culture medium environment with the NaCl concentration as high as 7%. Meanwhile, the strain also has the functions of dissolving organic phosphorus, dissolving potassium, producing amylase, producing ammonia, producing siderophore, producing auxin and the like. Greenhouse pot experiments show that the strain has a growth promoting effect on various crops such as wheat and corn, and the plant height, stem length, fresh weight and the like of plants can be remarkably improved. In addition, the strain has a good inhibition effect on rhizoctonia solani, pectobacterium and xanthomonas oryzae.

The application discloses application of florfenicol in prevention and treatment of plant bacterial diseases and an inhibitor. The application provides a new application of florfenicol, which can be used for preventing and treating Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. oryzae, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lactucae, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. angulata and the like, and provides a new idea for preventing and treating the above-mentioned plant bacterial diseases. Experiments prove that florfenicol has high bactericidal activity on the above-mentioned plant bacterial diseases.

The application discloses corn phosphatidyl phospholipase C4, a coding gene thereof and application of the corn phosphatidyl phospholipase C4 in enhancing disease resistance. The corn phosphatidyl phospholipase C4 can endow transgenic rice with wide resistance without affecting the yield of the rice. The amino acid sequence of the corn phosphatidyl phospholipase C4 is SEQ ID No.2. The sequence of the coding gene is SEQ ID No.1. Research shows that the corn phosphatidyl phospholipase C4 gene is located in the cell membrane, overexpression of the corn phosphatidyl phospholipase C4 gene in rice can improve the resistance of the rice to rice blast caused by Magnaporthe oryzae and rice bacterial leaf blight caused by Xanthomonas oryzae, and meanwhile, the agronomic characters of the rice are evaluated, and it is found that the gene does not affect the ear length, grain length, 1000-grain weight and yield per plant of the rice.

The invention discloses a synthesis method of an ester-substituted BCP aza-aromatic ring compound under red light heterogeneous catalysis, which comprises the following steps: by taking an aza-aromatic ring compound and ester-substituted BCP activated ester as raw materials, trifluoroacetic acid as an additive and mesoporous graphite phase carbon nitride as a catalyst, carrying out Minisci alkylation reaction on the ester-substituted BCP activated ester under red light heterogeneous catalysis to obtain the ester-substituted BCP aza-aromatic ring compound. A series of ester group substituted BCP aza aromatic ring compounds are simply, conveniently and efficiently synthesized. The method has the characteristics that the catalyst can be recycled, the reaction is mild and efficient, and the like, and the synthesized ester-substituted BCP aza-aromatic ring compound has bactericidal activity on Xanthomonas oryzae pv. Oryzicola Xoc and is expected to be used for creating green pesticides.

The invention provides a pleuromutilin derivative containing a thioether side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Pseudomonas solanacearum pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.