43 results about "Xanthomonas oryzae" patented technology

The invention relates to an application of carboxylated chitin or carboxylated chitosan in promoting insect-killing fungi to prevent and treat diseases and insect pests. Research finds that carboxylated chitin or carboxylated chitosan can significantly promote spore germination and hypha growth of insecticidal fungi, also can significantly enhance stress resistance of the insecticidal fungi, significantly enhance toxicity of the insecticidal fungi to pests, improve ultraviolet resistance and high temperature resistance of the insecticidal fungi, and improve the insecticidal activity of the insecticidal fungi. The invention also finds that the carboxylated chitin or chitosan can effectively inhibit the growth of Xanthomonas oryzae pv. Oryzae. Therefore, the carboxylated chitin or the carboxylated chitosan can be used for preparing a product for enhancing the insecticidal fungus effect and a product for preventing and treating the rice bacterial leaf blight, and the prevention and treatment of plant diseases and insect pests are realized. In addition, carboxylated chitin and carboxylated chitosan are derived from natural polymer materials, have the characteristics of safety, no toxicity, biodegradability, environmental friendliness and the like, are environment-friendly and economical, and have wide application prospects and market values.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention discloses leuconostoc mesenteroides a3 antagonizing xanthomonas oryzae and application of the leuconostoc mesenteroides a3, and belongs to the technical field of microorganisms. The leuconostoc mesenteroides a3 is preserved in the China Center for Type Culture Collection on April 7, 2025, the preservation number of the strain is CCTCC No: M 2025646, and the leuconostoc mesenteroides a3 has high antagonistic activity on rice bacterial leaf blight germs and rice bacterial leaf streak germs. The biocontrol microbial inoculum prepared from the strain has a good biocontrol effect on rice bacterial leaf blight and rice bacterial leaf streak, the use of chemical pesticides can be reduced, the safety of grains and the ecological environment can be improved, and good economic and social benefits can be brought.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method and application of five compounds (1-5) extracted from liquid fermentation of Chaetomium sp. Specifically, the present application discloses five compounds, wherein compound 1 is a new compound. The Chaetomium sp. metabolite has good antibacterial activity on three tested pathogenic bacteria (Pseudomonas syringae pv. actinidiae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola), and has important significance for development of a new type of microbial source antibacterial agent.

The invention discloses a rapid detection method for Xanthomonas oryzae pv. Oryzae on the basis of RPA-CRISPR / Cas12b. The reagent combination comprises a specific RPA primer pair and sgRNA, and the sequences of the specific RPA primer pair and the sgRNA are respectively shown as SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The detection method comprises the following steps: taking nucleic acid of a sample to be detected as a template, and performing RPA isothermal amplification by utilizing the primer pair; the amplification product is mixed with sgRNA, Cas12b protein and fluorescent reporter molecules for incubation, and the trans-cleavage activity of Cas12b is activated; a result is judged by detecting a fluorescence signal. According to the invention, the optimized RPA and CRISPR systems are integrated on the centrifugal micro-fluidic chip, so that the full-flow closed tube and automatic detection is realized, the detection can be completed within 30 minutes, the sensitivity reaches 0.156 pg / reaction, no non-specific reaction is caused to five related bacteria such as Xa and Xap, the sensitivity to XOO3 subtype is high, but the kit is not suitable for XOO2 subtype, and the kit has the advantages of rapidness, sensitivity, specificity and low equipment dependence degree, and can be used for detecting the related bacteria of XOO3 subtype and XOO2 subtype. The method is suitable for non-diagnostic detection of field environments, port environments and the like.

The invention discloses a composite nano preparation based on zinc sulfide quantum dots as well as a preparation method and application thereof, and relates to the technical field of nano agriculture and plant disease prevention and control. The nano preparation disclosed by the invention has high antibacterial efficiency, and the prepared ZnSQDs and the composite nano preparation of the ZnSQDs can show a remarkable inhibition effect on the Xanthomonas oryzae pv.oryzae (Xoo) under the condition of an extremely low dosage (2 to 10 mg / L), so that the ZnSQDs and the composite nano preparation of the ZnSQDs can be used for inhibiting the Xanthomonas oryzae pv.oryzae (Xoo) of the Xanthomonas oryzae pv.oryzae (Xoo); compared with the traditional chemical pesticide which can achieve the prevention and treatment effect only through the dosage of 100 milligrams per liter or even gram, the effective dosage of the compound is greatly reduced, and the compound shows ultrahigh antibacterial activity; meanwhile, due to the high specific surface area and active surface sites of the nano-material, the nano-material can be in full contact with pathogenic bacteria, and the action efficiency is improved, so that breeding and diffusion of the pathogenic bacteria can be effectively inhibited in the early stage, and diseases are remarkably reduced.

The application discloses application of a carbazole derivative in prevention and treatment of plant diseases and relates to the technical field of medicinal chemistry. The structure of the carbazole derivative is as follows: The carbazole derivative has excellent inhibiting effect on plant pathogenic bacteria such as Xanthomonas oryzae ACCC 11602, Xanthomonas axonopodis pv.Citri, Pseudomonas soli maceraum and Erwinia aroideae, and has the advantages of simple structure, cheap raw materials and easy synthesis, and has the value of further research and development.

The invention discloses an alkyl-substituted BCP phosphinate compound and a synthesis method and application thereof.According to the method, [1.1. 1] propeller alkane, Katritzky pyridinium salt and a trivalent organic phosphine compound serve as raw materials, bis [2-(2, 4-difluorophenyl)-5-trifluoromethylpyridine] [2-2 '-dipyridyl] iridium bis (hexafluorophosphonic acid) salt serves as a catalyst, and the alkyl-substituted BCP phosphinate compound is synthesized through a one-pot method. And a series of alkyl substituted BCP phosphinate compounds are simply, conveniently and efficiently synthesized by photocatalysis of free radical bifunctionalization reaction of [1.1. 1] propeller alkane. The synthesis method disclosed by the invention has the characteristics of simple and easily available raw materials, mild and efficient reaction and the like, and the synthesized alkyl substituted BCP phosphinate compound has bactericidal activity on rice plant pathogen xanthomonas oryzae pv. Oryzae Xoo and is expected to be used for creating green pesticides.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

The application belongs to the technical field of genetic transformation, and particularly relates to application of a lyase XooRuvC screened from a genome of Xanthomonas oryzae in improving resistance of rice to bacterial leaf blight. In the embodiment of the application, overexpression of the lyase XooRuvC in rice can significantly improve the resistance of rice to bacterial leaf blight without affecting normal growth and breeding of the crop, and is beneficial to cultivation of resistant varieties.

This invention discloses the promoter of the rice Os04g0650800 gene and its applications, belonging to the field of plant biotechnology. By cloning the promoter sequence of the Os04g0650800 gene, a recombinant expression vector pPGDH-YFP driving the yellow fluorescent protein gene was successfully constructed. Positive rice plants were screened by infecting Nipponbare rice with Agrobacterium. The T0 generation of positive rice seedlings were inoculated with Xanthomonas oryzae pv. oryzae. Xoo Using a confocal microscope, it was discovered that... Xoo Infection of positive rice plants can induce YFP expression. Genetic engineering of rice using this promoter, such as regulating the efficient expression of target genes through this promoter, will yield positive results. Xoo Infected rice plants; if this promoter is used to drive the expression of disease resistance-related genes in rice, rice plants resistant to bacterial blight will be obtained.

The structural formula of the thymol-containing quaternary ammonium salt derivative is shown as (1), wherein R1 is 2-isopropyl-5-methylphenyl, ethyl or a benzene ring, R2 is 2-chloro-5-methylthiophene, 3-methyl-2-methoxycarbonyl furan, 4-methylnaphthalene and differently substituted benzyl, X-is Cl-and Br-, and n is equal to 3, 5, 7, 8 and 9. The preparation method of the drug-loaded antibacterial liposome comprises the step of jointly assembling hydrogenated soybean phosphatidylcholine, cholesterol hemisuccinic acid monoester, phosphatidyl ethanolamine and a target compound thymol-containing quaternary ammonium salt derivative into the drug-loaded antibacterial liposome. Part of the compounds have excellent antibacterial activity on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri and Xanthomonas syringae pv. Kiwifruit. Meanwhile, the compound also has excellent biological activity on six fungi such as fusarium oxysporum and TMV viruses. A field test on Xoo shows that the protection effect of the drug-loaded antibacterial liposome is improved by 1.94 times, the treatment effect is improved by 1.56 times, and the drug-loaded antibacterial liposome shows remarkable pH-triggered release characteristics and colloidal stability. (1)

The invention belongs to the technical field of plant genetic engineering, and discloses a gene OSD4 participating in a synthetic route of PAL-dependent salicylic acid (SA) of rice and application of the gene OSD4. A mutant osd4 is obtained by knocking out OSD4 through CRISPR / Cas9, the SA content of leaves of the mutant osd4 is reduced to 15% of that of a wild type, and the mutant osd4 is more sensitive to xanthomonas oryzae pv. And constructing an OSD4 overexpression vector to obtain a transgenic material with enhanced resistance. Hormone determination and inoculation experiments show that OSD4 regulates SA synthesis and participates in disease-resistant reaction. The in-vitro enzyme activity is verified by constructing an OSD4-pMAL carrier, and it is proved that OSD4 can catalyze BS to directly generate SA. The gene can be used for disease resistance improvement of crops such as rice.

The invention discloses application of a SbHDA3 gene in regulation and control of plant disease resistance, and belongs to the technical field of gene engineering. The nucleotide sequence of the SbHDA3 gene is as shown in SEQ ID NO. 11. It is found that the SbHDA3 gene is remarkably related to plant disease resistance, the resistance of plants to xanthomonas oryzae, magnaporthe oryzae and colletotrichum gloeosporioides can be improved by overexpressing the gene, and the resistance is reduced if the gene is silent. The research result of the invention provides a new gene resource for plant disease-resistant breeding, and lays a theoretical foundation for researching a plant disease-resistant mechanism.

The invention provides a pleuromutilin derivative containing a thioacetate side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I

The invention provides a pleuromutilin derivative containing an amino side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The invention discloses a salt-tolerant nitrogen-fixing Klebsiella pasteurii and application thereof, and relates to the technical field of microorganisms, and the Klebsiella pasteurii S8812 provided by the invention is a salt-tolerant combined nitrogen-fixing bacterial strain which is screened from corn roots and has outstanding comprehensive performance; the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 21, 2025, and the preservation number of the strain is CGMCC No: 33923. The strain is high in nitrogen fixation activity, strong in salt resistance and capable of tolerating a culture medium environment with the NaCl concentration as high as 7%. Meanwhile, the strain also has the functions of dissolving organic phosphorus, dissolving potassium, producing amylase, producing ammonia, producing siderophore, producing auxin and the like. Greenhouse pot experiments show that the strain has a growth promoting effect on various crops such as wheat and corn, and the plant height, stem length, fresh weight and the like of plants can be remarkably improved. In addition, the strain has a good inhibition effect on rhizoctonia solani, pectobacterium and xanthomonas oryzae.

The application discloses application of florfenicol in prevention and treatment of plant bacterial diseases and an inhibitor. The application provides a new application of florfenicol, which can be used for preventing and treating Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. oryzae, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lactucae, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. angulata and the like, and provides a new idea for preventing and treating the above-mentioned plant bacterial diseases. Experiments prove that florfenicol has high bactericidal activity on the above-mentioned plant bacterial diseases.

The application discloses corn phosphatidyl phospholipase C4, a coding gene thereof and application of the corn phosphatidyl phospholipase C4 in enhancing disease resistance. The corn phosphatidyl phospholipase C4 can endow transgenic rice with wide resistance without affecting the yield of the rice. The amino acid sequence of the corn phosphatidyl phospholipase C4 is SEQ ID No.2. The sequence of the coding gene is SEQ ID No.1. Research shows that the corn phosphatidyl phospholipase C4 gene is located in the cell membrane, overexpression of the corn phosphatidyl phospholipase C4 gene in rice can improve the resistance of the rice to rice blast caused by Magnaporthe oryzae and rice bacterial leaf blight caused by Xanthomonas oryzae, and meanwhile, the agronomic characters of the rice are evaluated, and it is found that the gene does not affect the ear length, grain length, 1000-grain weight and yield per plant of the rice.

The invention discloses a synthesis method of an ester-substituted BCP aza-aromatic ring compound under red light heterogeneous catalysis, which comprises the following steps: by taking an aza-aromatic ring compound and ester-substituted BCP activated ester as raw materials, trifluoroacetic acid as an additive and mesoporous graphite phase carbon nitride as a catalyst, carrying out Minisci alkylation reaction on the ester-substituted BCP activated ester under red light heterogeneous catalysis to obtain the ester-substituted BCP aza-aromatic ring compound. A series of ester group substituted BCP aza aromatic ring compounds are simply, conveniently and efficiently synthesized. The method has the characteristics that the catalyst can be recycled, the reaction is mild and efficient, and the like, and the synthesized ester-substituted BCP aza-aromatic ring compound has bactericidal activity on Xanthomonas oryzae pv. Oryzicola Xoc and is expected to be used for creating green pesticides.

The invention provides a pleuromutilin derivative containing a thioether side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Pseudomonas solanacearum pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The application discloses a bacteriophage E12-2 of Xanthomonas oryzae and application thereof, and belongs to the technical field of microorganisms. The bacteriophage is obtained by using Xanthomonas oryzae with bacteriophage resistance for multiple rounds of directional evolution, is named Xanthomonas oryzae phage E12-2, and is preserved in the China Center for Type Culture Collection on March 17, 2025, has a preservation address of No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and a preservation number of CCTCC NO: M 2025486. The bacteriophage can lyse common Xanthomonas oryzae and resistant strains thereof, has a wide host spectrum, strong lytic activity, and a wide application prospect in the prevention and treatment of Xanthomonas oryzae diseases.

The application discloses a target for detecting and identifying Xanthomonas oryzae pv. oryzae (Xoo), which is a nucleotide sequence as shown in SEQ ID No. 1. The application also discloses a primer pair for detecting and identifying Xoo, which comprises a forward primer 5'-CCTTCTGCAATCTATGGCACC-3' and a reverse primer 5'-TAAATAACACGGTGAAGCCGAC-3'. The primer pair can be used for PCR and can only amplify a nucleic acid fragment with a length of 138 bp from a sample containing Xoo DNA, has specificity and universality for detecting and identifying Xoo, and realizes accurate detection and identification of Xoo. The primer pair is suitable for real-time fluorescent quantitative PCR, and the detection sensitivity reaches 2.7x10 2 fg / μL.

The invention discloses application of 2, 6-dicarboxypyridine in inhibition of growth of plant pathogenic bacteria, and belongs to the technical field of biological agriculture. The invention firstly discovers that the 2, 6-dicarboxyl pyridine can be used for inhibiting the growth of Xanthomonas plant pathogenic bacteria, especially Xanthomonas carpesium xanthomonas soybean pathogenic variants (Xanthomonas citrulli pv.glycines, Xcg), Xanthomonas oryzae pv.oryzae, Xoo, Xanthomonas oryzae pv.oryzae, Xoc, Xanthomonas campestris pv.oryzae pv.oryzae, Xanthomonas campestris pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae Xcc), and can be used for preparing pesticides for preventing and treating plant pathogenic bacteria.

The application belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or its coding gene in improving broad-spectrum disease resistance and regulating agronomic traits of rice. The application constructs an OsDOF3 knockout mutant and overexpression transgenic line in a susceptible rice background, and through inoculation of various pathogenic bacteria (including Magnaporthe oryzae, Rhizoctonia solani and Xanthomonas oryzae), resistance phenotype analysis is carried out, and it is confirmed that OsDOF3 participates in regulating the immune response pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (white leaf blight). Meanwhile, it is found that after being driven by a strong promoter ubi, the overexpression material of OsDOF3 is shorter than the wild type plant, and OsDOF3 affects the normal growth and development of rice while triggering the immune response of the plant. The application provides important gene resources and molecular theoretical basis for developing new varieties of broad-spectrum disease-resistant rice.

The invention discloses a preparation method and application of a benzyl indolyl diphenylphosphine derivative, the structural general formula of the benzyl indolyl diphenylphosphine derivative is shown as follows: the benzyl indolyl diphenylphosphine derivative is obtained by using iodobenzene to initiate a free radical mediated anion transfer phosphorylation process; the compound is applied to the aspect of inhibiting kiwifruit canker pathogen and rice bacterial blight pathogen. Compared with the prior art, the benzyl indolyl diphenylphosphine derivative has obvious beneficial effects, the synthesized benzyl indolyl diphenylphosphine derivative is not disclosed, and part of compounds have certain bacteriostatic activity.

The invention provides bacillus vallismortis TCS004 with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.25850. The invention discloses an application of the bacillus vallismortis TCS004 in preventing and treating plant pathogenic fungi or plant pathogenic bacteria, or an application of the bacillus vallismortis TCS004 in preparing a microbial preparation for preventing and treating the plant pathogenic fungi or the plant pathogenic bacteria. The bacterial strain has a very high inhibition effect on plant pathogenic fungi such as lasiodiplodia mali, macrostem botryosphaeria verticillata, citrus interspersonia, streptosclerotinia sclerotiorum, alternaria alternata, colletotrichum frutescens, muscardine fungus, alternaria solani, fusarium solani, botrytis cinerea, fusarium equisetum, curvularia, alternaria alternata and the like; and the compound also has relatively good bacteriostatic activity on plant pathogenic bacteria xanthomonas oryzae and the like. The invention further provides a formula of a fermentation culture medium of the bacillus vallismortis TCS004 and fermentation process conditions of the bacillus vallismortis TCS004.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.

The application discloses a Paraconiothyrium cyclothyrioides PF432 and a preparation method and application of metabolites thereof. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20252943. Through liquid fermentation and chromatographic separation, 32 compounds, i.e. compounds 1-21, 22-29 and 30-32, are obtained from fermentation products of ME culture medium, PD culture medium and Gao's No. 1 culture medium of the strain, and the structures are identified through NMR, MS and single crystal X-ray diffraction analysis. Active tests show that the compounds have significant inhibiting effects on Xanthomonas oryzae pv. oryzae and Xanthomonas campestris pv. oryzae, wherein the in-vivo prevention and treatment effects of compounds 10, 15 and 16 on Xanthomonas oryzae pv. oryzae at a concentration of 500 ng / mL are better than that of positive control milbemycins. The application provides new candidate compounds for green prevention and control of plant bacterial diseases.