22 results about "Xanthomonas oryzae" patented technology

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method and application of five compounds (1-5) extracted from liquid fermentation of Chaetomium sp. Specifically, the present application discloses five compounds, wherein compound 1 is a new compound. The Chaetomium sp. metabolite has good antibacterial activity on three tested pathogenic bacteria (Pseudomonas syringae pv. actinidiae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola), and has important significance for development of a new type of microbial source antibacterial agent.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

The invention belongs to the technical field of plant genetic engineering, and discloses a gene OSD4 participating in a synthetic route of PAL-dependent salicylic acid (SA) of rice and application of the gene OSD4. A mutant osd4 is obtained by knocking out OSD4 through CRISPR / Cas9, the SA content of leaves of the mutant osd4 is reduced to 15% of that of a wild type, and the mutant osd4 is more sensitive to xanthomonas oryzae pv. And constructing an OSD4 overexpression vector to obtain a transgenic material with enhanced resistance. Hormone determination and inoculation experiments show that OSD4 regulates SA synthesis and participates in disease-resistant reaction. The in-vitro enzyme activity is verified by constructing an OSD4-pMAL carrier, and it is proved that OSD4 can catalyze BS to directly generate SA. The gene can be used for disease resistance improvement of crops such as rice.

The invention provides a pleuromutilin derivative containing a thioacetate side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I

The invention provides a pleuromutilin derivative containing an amino side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The application discloses application of florfenicol in prevention and treatment of plant bacterial diseases and an inhibitor. The application provides a new application of florfenicol, which can be used for preventing and treating Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. oryzae, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lactucae, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. angulata and the like, and provides a new idea for preventing and treating the above-mentioned plant bacterial diseases. Experiments prove that florfenicol has high bactericidal activity on the above-mentioned plant bacterial diseases.

The application discloses corn phosphatidyl phospholipase C4, a coding gene thereof and application of the corn phosphatidyl phospholipase C4 in enhancing disease resistance. The corn phosphatidyl phospholipase C4 can endow transgenic rice with wide resistance without affecting the yield of the rice. The amino acid sequence of the corn phosphatidyl phospholipase C4 is SEQ ID No.2. The sequence of the coding gene is SEQ ID No.1. Research shows that the corn phosphatidyl phospholipase C4 gene is located in the cell membrane, overexpression of the corn phosphatidyl phospholipase C4 gene in rice can improve the resistance of the rice to rice blast caused by Magnaporthe oryzae and rice bacterial leaf blight caused by Xanthomonas oryzae, and meanwhile, the agronomic characters of the rice are evaluated, and it is found that the gene does not affect the ear length, grain length, 1000-grain weight and yield per plant of the rice.

The invention provides a pleuromutilin derivative containing a thioether side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Pseudomonas solanacearum pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The application discloses a bacteriophage E12-2 of Xanthomonas oryzae and application thereof, and belongs to the technical field of microorganisms. The bacteriophage is obtained by using Xanthomonas oryzae with bacteriophage resistance for multiple rounds of directional evolution, is named Xanthomonas oryzae phage E12-2, and is preserved in the China Center for Type Culture Collection on March 17, 2025, has a preservation address of No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and a preservation number of CCTCC NO: M 2025486. The bacteriophage can lyse common Xanthomonas oryzae and resistant strains thereof, has a wide host spectrum, strong lytic activity, and a wide application prospect in the prevention and treatment of Xanthomonas oryzae diseases.

The application discloses a target for detecting and identifying Xanthomonas oryzae pv. oryzae (Xoo), which is a nucleotide sequence as shown in SEQ ID No. 1. The application also discloses a primer pair for detecting and identifying Xoo, which comprises a forward primer 5'-CCTTCTGCAATCTATGGCACC-3' and a reverse primer 5'-TAAATAACACGGTGAAGCCGAC-3'. The primer pair can be used for PCR and can only amplify a nucleic acid fragment with a length of 138 bp from a sample containing Xoo DNA, has specificity and universality for detecting and identifying Xoo, and realizes accurate detection and identification of Xoo. The primer pair is suitable for real-time fluorescent quantitative PCR, and the detection sensitivity reaches 2.7x10 2 fg / μL.

The application belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or its coding gene in improving broad-spectrum disease resistance and regulating agronomic traits of rice. The application constructs an OsDOF3 knockout mutant and overexpression transgenic line in a susceptible rice background, and through inoculation of various pathogenic bacteria (including Magnaporthe oryzae, Rhizoctonia solani and Xanthomonas oryzae), resistance phenotype analysis is carried out, and it is confirmed that OsDOF3 participates in regulating the immune response pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (white leaf blight). Meanwhile, it is found that after being driven by a strong promoter ubi, the overexpression material of OsDOF3 is shorter than the wild type plant, and OsDOF3 affects the normal growth and development of rice while triggering the immune response of the plant. The application provides important gene resources and molecular theoretical basis for developing new varieties of broad-spectrum disease-resistant rice.

The invention discloses a preparation method and application of a benzyl indolyl diphenylphosphine derivative, the structural general formula of the benzyl indolyl diphenylphosphine derivative is shown as follows: the benzyl indolyl diphenylphosphine derivative is obtained by using iodobenzene to initiate a free radical mediated anion transfer phosphorylation process; the compound is applied to the aspect of inhibiting kiwifruit canker pathogen and rice bacterial blight pathogen. Compared with the prior art, the benzyl indolyl diphenylphosphine derivative has obvious beneficial effects, the synthesized benzyl indolyl diphenylphosphine derivative is not disclosed, and part of compounds have certain bacteriostatic activity.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.

The application discloses a Paraconiothyrium cyclothyrioides PF432 and a preparation method and application of metabolites thereof. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20252943. Through liquid fermentation and chromatographic separation, 32 compounds, i.e. compounds 1-21, 22-29 and 30-32, are obtained from fermentation products of ME culture medium, PD culture medium and Gao's No. 1 culture medium of the strain, and the structures are identified through NMR, MS and single crystal X-ray diffraction analysis. Active tests show that the compounds have significant inhibiting effects on Xanthomonas oryzae pv. oryzae and Xanthomonas campestris pv. oryzae, wherein the in-vivo prevention and treatment effects of compounds 10, 15 and 16 on Xanthomonas oryzae pv. oryzae at a concentration of 500 ng / mL are better than that of positive control milbemycins. The application provides new candidate compounds for green prevention and control of plant bacterial diseases.

The invention relates to the technical field of chemical synthesis and application, in particular to a 1, 4-naphthoquinone derivative and a preparation method and application thereof. The 1, 4-naphthoquinone derivative is prepared by carrying out mercaptan addition on sulfydryl of a sulfydryl-containing compound and 1, 4-naphthoquinone at a site 2, the preparation method is simple, antibacterial activity results show that the prepared 1, 4-naphthoquinone derivative has antibacterial activity on xanthomonas oryzae, pseudomonas syringae and ralstonia solanacearum, and the antibacterial effect is higher than that of kasugamycin.

The application discloses a quinina derivative for preventing and treating agricultural diseases, a preparation method and application thereof, and relates to a compound with a structural formula as shown in formula I, or a pharmaceutically acceptable salt, a solvate and a tautomer thereof. It is found that the quinina derivative has excellent inhibitory effect on Xanthomonas oryzae and Xanthomonas citri, has great development value in treating plant pathogenic bacteria, and provides more choices for preventing and treating agricultural diseases.

The invention provides a pleuromutilin derivative containing an ester side chain or a silicon-oxygen side chain and application of the pleuromutilin derivative to prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

This invention discloses the application of a kojic acid derivative containing a thioether structure in the preparation of a fungicide for controlling plant bacterial diseases. The compound has the following general structural formula: R is a substituted phenyl group, and the substituent is fluorine, chlorine, bromine, methyl, methoxy, amino, or nitro. The focus of this invention is that this type of compound exhibits excellent biological activity in controlling plant bacterial diseases, especially against rice bacterial blight (… Xanthomonas oryzae pv. oryzae It exhibits significant inhibitory effects, with some compounds showing superior activity compared to commercial agents such as thiabendazole and tebuconazole. The preparation process is simple and suitable for widespread application.

The application belongs to the field of pharmaceutical chemistry, and discloses a new use of a diaryl ether-pyrazole heterocyclic compound in preventing and treating agricultural pathogenic fungal diseases caused by Botrytis cinerea Pers., Fusarium graminearum Schw., Rhizoctonia solani, Phytophthora capsici Leonian., Sclerotinia sclerotiorum, and in preventing and treating plant diseases caused by Xanthomonas oryzae ACCC 11602, Xanthomonas axonopodis pv.Citri, Pseudomonas soli macerae and Erwinia aroideae, and diseases caused by human pathogenic bacteria Escherichia coli ATCC 25922, Staphyloccocus aureus sp.Newman and Candida albicans ATCC 24433. The structural general formula of the diaryl ether-pyrazole heterocyclic compound is as follows: the diaryl ether-pyrazole heterocyclic compound has the characteristics of simple structure, easy synthesis, low price and wide use, and through activity determination, it is found that the diaryl ether-pyrazole heterocyclic compound has clear structure-activity relationship, a wide bactericidal spectrum and further research and development value.

This invention provides a method for detecting genes that resist bacterial blight in rice. Xa21 The InDel marker for genotypes and its application belongs to the field of rice breeding technology. The upstream primer sequence for the InDel marker is Xa21-InDel-F: 5'-TCTGCTCTACACTGCAATATGAAATGA-3', and the downstream primer sequence is Xa21-InDel-R: 5'-TAGTACCAAGTATTTTTCATGGTCAATG-3'. The Xa21-InDel marker was used to detect IRBB21 and 37 hybrid japonica rice restorer lines. The results showed that the InDel marker designed in this invention can accurately identify whether different varieties contain genes for resistance to bacterial blight. Xa21 And heterozygous genotype. This detection method has high accuracy and specificity. Xa21 Molecular marker-assisted selection of genes has strong practical applications in breeding.

The application belongs to the field of pharmaceutical chemistry, and discloses an application of a compound with a diaminopyrroloquinazoline skeleton in preventing and treating plant bacterial diseases caused by Xanthomonas oryzae ACCC 11602, Xanthomonas axonopodis pv.Citr, Pseudomonas sollamacearum and Erwinia aroideae. The structural general formula is as follows: wherein R is an alkyl group, a para-substituted phenyl group, an ortho-substituted phenyl group or a meta-substituted phenyl group; A is hydrogen, a methyl group, an isopropyl group, a phenyl group, a methoxy group, a trifluoromethoxy group, a trifluoromethyl group, halogen or a cyano group; B is hydrogen or halogen; and C is hydrogen or halogen.