Method for detecting Xanthomonas oryzae pv.oryzae, Xoo, X.oryzae pv. Oryzicola, Xoox, X.axonopodis pv. Citri, Xac, and X.campestris pv.campestris, Xcc

Abstract

The invention discloses a method for detecting Xanthomonas oryzae pv.oryzae, Xoo, X.oryzae pv. Oryzicola, Xoox, X.axonopodis pv. Citri, Xac, and X.campestris pv.campestris, Xcc. The method comprises the following steps: carrying out PCR (polymerase chain reaction) amplification on the DNA template of a sample to be detected by using two pairs of primers at the same time; then, hybridizing the PCR products with the specific probe arranged on a gene chip; and judging whether the sample to be detected is with Xanthomonas oryzae pv.oryzae, Xoo, X.oryzae pv. Oryzicola, Xoox, X.axonopodis pv. Citri, Xac, and X.campestris pv.campestris, Xcc according to the fact whether the hybridization result is positive or not. Therefore, the method of the invention is capable of detecting four bacteria by one time with high detection speed and has the advantages of direct-viewing and clear detection result and easy judgment.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to rapid detection techniques for rice bacterial blight, rice bacterial streak, citrus canker and cabbage black rot. Background technique [0002] The genus Xanthomonas has a wide variety of pathogenicity. The second edition of "Bergey's Handbook of Systematic Bacteriology" included 20 species, 70 pathogenic species with confirmed taxonomic status and 70 taxonomic status not yet identified pathogenic variants. There are 14 quarantine Xanthomonas species included in the current "List of Imported Plant Quarantine Pests of the People's Republic of China". Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xooc) are important bacterial diseases of rice. Rice bacterial blight can cause rice yield reduction by 10% to 30%, and in severe cases it can reach more than 50%. Bacterial spot of rice can cause rice yield reduction by 5% to 10%, and even up to 20% in se...

AI Technical Summary

Problems solved by technology

Physiological and biochemical methods, the operation steps are cumbersome, and it takes about 15 days to identify one kind of bacteria, which is too time-consuming, and the relevant personnel need rich experience

Based on the molecular biology of PCR technology, there are mainly common PCR, double PCR, real-time fluorescent PCR and rolling circle amplification methods for the detection of Xanthomonas; these methods are prone to pollution, low sensitivity, and can only detect one species at a time. Insufficient pathogenic bacteria, etc.

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