Bacillus velezensis 229-15 and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of microorganisms, can solve the problems of ecological environment damage, lack of disease-resistant varieties, etc., and achieve the effect of broad-spectrum antibacterial ability and good antagonistic effect

Active Publication Date: 2020-06-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of agricultural production, the prevention and control of these two diseases still has great limitations. In addition to the lack of good and stable disease-resistant varieties, the long-term use of chemical reagents has caused certain damage to the ecological environment. Approaches to environment and disease control are urgently needed

Method used

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  • Bacillus velezensis 229-15 and application thereof
  • Bacillus velezensis 229-15 and application thereof
  • Bacillus velezensis 229-15 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1, Isolation, screening and purification of Bacillus veleisi 229-15

[0050] 1. Source of the strain

[0051] The pathogenic strains used are all strains preserved by our laboratory, and all of them are published strains.

[0052] 2. Acquisition of antagonistic strains

[0053] (1) Discovery of antagonistic strains

[0054] In the laboratory, a strain capable of inhibiting the growth of bacterial blight of rice was screened from campus soil samples collected by Gansu Agricultural University in Anning District, Gansu Province, China.

[0055] (2) Purification and preservation of strains

[0056] Using plate streaking method: Pick out the colony that can inhibit the growth of bacterial blight, streak on a new NA plate, place it in a biochemical incubator at 28°C, and culture it upside down for more than 12 hours, pick a single colony, and number it for 229-15. Inoculate the strain 229-15 in the NA liquid medium, culture it in a shaker at 28°C and 180rpm for 1...

Embodiment 2

[0059] 16S rRNA gene identification of embodiment 2, bacterial strain 229-15

[0060] Use bacterial universal primer 27F 5'-AGAGTT TGA TCCTGGCTCAG-3' and 1492R 5'-TACGGCTACCTTGTTACGACTT-3', and using the genomic DNA of strain 229-15 as a template; prepare a 30 μL PCR reaction system according to the ratio in Table 1, and perform the PCR reaction:

[0061] Table 1 Taq polymerase chain reaction system

[0062]

[0063] The basic conditions of the PCR reaction were set as follows: pre-denaturation at 95°C for 5 min; denaturation at 95°C for 30 s, annealing at 56°C for 30 s, extension at 72°C for 90 s, a total of 30 cycles; pre-extension at 72°C for 10 min. After the PCR reaction, the amplified PCR product of strain 229-15 was tested by 1% agarose gel electrophoresis, and the image was detected by a gel imager, and then the original solution of the PCR product was sent to Bosun Biotechnology (Shanghai) Co., Ltd. sequencing.

[0064]The obtained sequencing results were analy...

Embodiment 3

[0066] Embodiment 3, the morphology and physiological and biochemical identification of bacterial strain 229-15

[0067] Bacterial strain 229-15 morphological observation of the present invention, such as figure 1 As shown, the colony of bacterial strain 229-15 was found to be milky white, with rough and irregular edges, dry and rough surface, and opaque; the result of Gram staining was positive. The physiological and biochemical test results of the bacterial strain SA001 of the present invention show that the bacterial strain of the table has enzymatic activity to pyruvate and Kohn gelatin; but it cannot oxidize glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, honey Sugar, amygdalin and arabinose; 17 kinds of carbon sources such as glycerin, L-arabinose and D-xylose can be used to generate acid. (See Table 2 and Table 3).

[0068] Table 2 Physiological and biochemical characteristics of strain 229-15 - enzyme activity, carbon source oxidation

[0069]

[0070] ...

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Abstract

The invention relates to Bacillus velezensis 229-15 and application thereof. The Bacillus velezensis is preserved in the Chinese Type Culture Collection on September 20, 2019, the sample name is Bacillus 229-15 Bacillus sp. 229-15, the preservation number is CCTCC No: CCTCC M 2019734, and the strain is isolated from a campus soil sample in Gansu Agricultural University, Anning District, Gansu Province in China. The Bacillus velezensis 229-15 has a bacteriostatic effect on Xanthomonas oryzae pv.oryzae, Xoo and Xanthomonas oryzae pv.oryzicola, Xoc, and also has bacteriostatic effects on X.campestris pv.musacearum, X.axonopodis pv.vignicola, X.campestris pv.phaseoli and Magnaporthe oryzae, Botrytis cinerea, Fusarium graminearum and other pathogenic fungi at the same time. It is shown throughresults that the strain has a broad-spectrum bacteriostatic ability, good biocontrol resources are provided for prevention and control of Xanthomonas oryzae pv.oryzae, Xoo and Xanthomonas oryzae pv.oryzicola, Xoc, and a foundation is laid for development of novel microbial agents.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of bacillus velesi and its application. Background technique [0002] Rice is a very important food crop and economic crop in my country, and nearly half of the world's population now uses rice as a staple food. Therefore, ensuring the yield and quality of rice is of great significance to ensuring food security both globally and in my country. Rice bacterial leaf blight (BLB) and rice streak (BLS) are two diseases caused by two pathogenic species of Xanthomonas oryzae. It is a very important bacterial disease, which seriously restricts the production safety of rice grain in my country. [0003] Rice bacterial blight is one of the three major diseases of rice in China. It occurs more severely in East China, Central China, and South China. It is also the most serious bacterial disease in rice production in the world. It occurs in all rice regions around the wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
CPCA01N63/00C12R2001/07C12N1/205
Inventor 邹丽芳黄梦桑李生樟杨瑞环陈路生屈伊凝陈功友
Owner SHANGHAI JIAO TONG UNIV
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