Burkholderia cepacia, and cultivation method and application thereof

A technology of Holderella and strains, applied in the field of bidirectional Burkholderia and its cultivation, can solve the problems of lack of biological control drugs, large side effects of chemical control of plant diseases, etc., and achieve resistance not easy to develop. , Strong inhibitory effect, promising effect

Inactive Publication Date: 2013-12-11
西安西诺农化有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method that can inhibit the growth of plant diseases including banana anthracnose bacteria and rice sheath blight bacteria in the prior art, in view of

Method used

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  • Burkholderia cepacia, and cultivation method and application thereof
  • Burkholderia cepacia, and cultivation method and application thereof
  • Burkholderia cepacia, and cultivation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Screening and Identification of Bidirectional Burkholderia SD7

[0020] 1. Screening of bacterial strains

[0021] (1) Collection of soil samples

[0022] In this experiment, soil samples were collected from the farm orchard of South China Agricultural University. According to the topography of the collection site, 5 points were used for sampling. The sampling depth was 15cm below the plow layer or the surface, and the sampling volume was 200-250g. The 5 samples collected at each location were evenly mixed. , put into a sterilized sealed polyethylene bag, bring it back to the laboratory and separate it immediately.

[0023] (2) Preparation of sample suspension

[0024] Add the soil sample into sterile water and shake vigorously. Sample: water = 1:9 (mass to volume ratio), such as: 10g of soil is added to a conical flask filled with 90mL of sterilized water to obtain a suspension concentration of 10 -1 . Prepare 5 test tubes containing 9 mL of sterilized w...

Embodiment 2

[0040] Example 2 Inhibitory effect of bidirectional Burkholderia SD7 on plant pathogenic bacteria

[0041] Control Effects of Burkholderia bidirectionalum SD7 on Banana Fruit Anthracnose

[0042] (1) Tested banana anthracnose strains

[0043] The banana anthracnose bacterium (C. musae) used for inoculation was isolated and purified from the diseased banana fruit, and was preserved by our laboratory.

[0044] (2) Control effect of bidirectional Burkholderia SD7 on banana fruit anthracnose

[0045] Inoculate 1 mL of 2-day-old Burkholderia bidirectional SD7 strain seeds per 100 mL of LB medium (peptone: 10 g / L, yeast extract powder: 5 g / L, etc., NaCl: 10 g / L, pH: 7.0-7.2) Proportional seed inoculation and expansion culture, the culture conditions are: rotation speed 200 r / min, 25-30 ℃ culture for 20-24 hours, centrifuged to collect the supernatant, filtered with a bacterial filter to obtain a sterile filtrate. Select seven or eight mature bananas, disinfect the surface with 70...

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Abstract

The invention relates to burkholderia cepacia, and a cultivation method and an application thereof, and belongs to the field of plant protection (microbial pesticide). A preservation number of the burkholderia cepacia is CGCC No. 5384. According to the cultivation method, fermentation conditions are as follows: an LB culture medium is used; liquid volume is 50 mL; 1 mL of 2-day old seed strain is added; and the strain is cultured for 20-24 h at a temperature of 25-30 DEG C and at a rotation speed being 200 r/min. The burkholderia cepacia has significant inhibition effects for rhizoctonia solani, pyricularia oryzae, collectotrichum musae, fusarium oxysporum f. sp. cubense, gibberella zeae, peronophythora litchi, ceratocystis adiposum, fulvia fulva, xanthomonas oryzae pv. oryzae, xanthomonas oryzae pv. oryzicola and the like, can be used for biological control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environment friendliness and the like, and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of plant protection (microbial pesticides), and in particular relates to a bidirectional Burkholderia and its cultivation method and application. Background technique [0002] Plant diseases caused by plant pathogenic bacteria (including fungi, bacteria, viruses, etc.) have caused significant economic losses to agricultural production. At present, the main method of preventing and treating important plant diseases such as rice and bananas is to use fungicides. However, the extensive use of fungicides has the risk of increasing the resistance of pathogenic bacteria, causing serious pollution to the environment, and posing a threat to the health of humans and animals. Biological control is a new way to prevent and control plant diseases that is environmentally friendly. [0003] There are not only pathogenic bacteria around plants, but also a large number of non-pathogenic bacteria, some of which are antagonistic micro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/01
Inventor 杨忠绪张小荣
Owner 西安西诺农化有限责任公司
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