Burkholderia cepacia, and cultivation method and application thereof
A technology of Holderella and strains, applied in the field of bidirectional Burkholderia and its cultivation, can solve the problems of lack of biological control drugs, large side effects of chemical control of plant diseases, etc., and achieve resistance not easy to develop. , Strong inhibitory effect, promising effect
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Embodiment 1
[0019] Example 1 Screening and Identification of Bidirectional Burkholderia SD7
[0020] 1. Screening of bacterial strains
[0021] (1) Collection of soil samples
[0022] In this experiment, soil samples were collected from the farm orchard of South China Agricultural University. According to the topography of the collection site, 5 points were used for sampling. The sampling depth was 15cm below the plow layer or the surface, and the sampling volume was 200-250g. The 5 samples collected at each location were evenly mixed. , put into a sterilized sealed polyethylene bag, bring it back to the laboratory and separate it immediately.
[0023] (2) Preparation of sample suspension
[0024] Add the soil sample into sterile water and shake vigorously. Sample: water = 1:9 (mass to volume ratio), such as: 10g of soil is added to a conical flask filled with 90mL of sterilized water to obtain a suspension concentration of 10 -1 . Prepare 5 test tubes containing 9 mL of sterilized w...
Embodiment 2
[0040] Example 2 Inhibitory effect of bidirectional Burkholderia SD7 on plant pathogenic bacteria
[0041] Control Effects of Burkholderia bidirectionalum SD7 on Banana Fruit Anthracnose
[0042] (1) Tested banana anthracnose strains
[0043] The banana anthracnose bacterium (C. musae) used for inoculation was isolated and purified from the diseased banana fruit, and was preserved by our laboratory.
[0044] (2) Control effect of bidirectional Burkholderia SD7 on banana fruit anthracnose
[0045] Inoculate 1 mL of 2-day-old Burkholderia bidirectional SD7 strain seeds per 100 mL of LB medium (peptone: 10 g / L, yeast extract powder: 5 g / L, etc., NaCl: 10 g / L, pH: 7.0-7.2) Proportional seed inoculation and expansion culture, the culture conditions are: rotation speed 200 r / min, 25-30 ℃ culture for 20-24 hours, centrifuged to collect the supernatant, filtered with a bacterial filter to obtain a sterile filtrate. Select seven or eight mature bananas, disinfect the surface with 70...
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