Eye wash with moisturizing, antibacterial and antioxidant effects and preparation method thereof

Abstract

The invention discloses eye wash with moisturizing, antibacterial and antioxidant effects and a preparation method of the eye wash, and the eye wash comprises the following components in percentage by mass: 0.1-5% of a dendrobium nobile extract; 0.1%-2% of a Japan cypress leaf extract; 0.001 to 0.005 percent of a freshener; 0.1 to 3.0% of a humectant; 0.5 to 5.0 percent of a pH value regulator; 0.4-6.0% of an osmotic pressure regulator; and the balance of purified water. The preparation method comprises the following steps: weighing the dendrobium nobile extract, the chamaecyparis obtusa leaf extract, the freshener and the humectant according to the formula ratio, adding part of purified water, and uniformly stirring and mixing; the pH is adjusted to 6.8-7.8, the osmotic pressure is 260-340 mOsm / kg H2O, finally, the balance of purified water is added, the materials are stirred and mixed to be uniform, then filtration sterilization is conducted, filtrate is taken and subpackaged, and the eye washing liquid is obtained. Polyphenols in the dendrobium nobile extract and hinokitiol in the chamaecyparis obtusa leaf extract have a synergistic effect in antioxidant and antibacterial aspects, hinokitiol has an antibacterial effect on various bacteria, polyphenol also has antioxidant and antibacterial characteristics, and the antibacterial effect and the antioxidant effect can be enhanced by combining the polyphenol and the hinokitiol.

Description

Technical Field

[0001] The invention belongs to the technical field of eye health care, and in particular is an eyewash liquid with moisturizing, antibacterial and antioxidant effects and a preparation method thereof. Background Art

[0002] With the popularity of electronic products and the increase in the frequency of use, dry eyes have become a common problem. Therefore, eyewash liquid came into being. It has the functions of cooling and relieving itching, promoting eye nutrition, keeping eyes moist and hygienic, and avoiding conjunctival congestion. It is generally used to prevent and reduce eye discomfort and dry eyes caused by wearing contact lenses or eye mucus; it can also be used to relieve discomfort such as conjunctival congestion, dryness, astringency, itching, and pain caused by beauty, swimming, dust, powder or sweat entering the eyes; it can gently clean the eyes, replenish moisture to the eyes, relieve dryness, and prevent eye infections.

[0003] However, it is understood that the main active ingredients of commercially available eyewashes include: ampheniramine maleate, sodium chondroitin sulfate, dipotassium glycyrrhizinate, vitamin B6, taurine, vitamin B12, etc., which seem to have diverse ingredients and rich nutrition, but there are great hidden dangers. For example: the side effect of sodium chondroitin sulfate is occasional mild irritation, and long-term or frequent use can cause drug-induced keratitis, etc.; and frequent large-scale supplementation of vitamin B6 may lead to severe peripheral neuritis. Furthermore, in order to extend the shelf life, preservatives are added to eyewashes, which can damage the ocular surface in the following ways: destroying the microvilli of the corneal epithelium; having a detergent-like effect on the lipid layer, accelerating the evaporation of tears; indirectly destroying the stability of the tear film; reducing the growth and vitality of corneal cells; leading to inflammatory reactions on the ocular surface, subconjunctival fibrosis, etc. In addition, even if preservatives are added, bacterial contamination of eyewashes cannot be completely avoided due to factors such as the type, concentration, compatibility and use of preservatives.

[0004] In addition, anti-allergic drugs are added to eyewashes, such as ampheniramine maleate (commonly known as chlorpheniramine). Although its content is generally not high, frequent use will cause drug dependence. Improper use of eyewashes, eye drops, etc. can easily destroy the flora environment of the ocular surface, leading to dysbiosis, thereby increasing the risk of ocular surface diseases.

[0005] In order to solve the technical problem that the ingredients in the above-mentioned commercially available eyewashes cause different degrees of eye damage, some plant extract ingredients are widely used in eyewashes to play antibacterial, anti-inflammatory, moisturizing and other effects, such as schizophyllan (CN202111617935.0), ginsenosides (CN201911041542.2), trehalose (CN202111551410.1), borneol extract (CN202111110505.X), tea polyphenols (CN201811057304.6), super concentrated extract of ophthalmic herb, super concentrated extract of pearl, super concentrated extract of blueberry (CN201911171129.8), etc., but there are currently no reports on the use of Dendrobium nobile extract and Japanese cypress leaf extract in eyewashes. Summary of the invention

[0006] Based on the above, the purpose of the present invention is to provide an eyewash with moisturizing, antibacterial and antioxidant effects and a preparation method thereof, which uses natural ingredients such as Dendrobium nobile extract and Japanese cypress leaf extract to achieve the moisturizing, antibacterial and antioxidant functions of the eyes.

[0007] To achieve its purpose, the present invention adopts the following technical solution: An eyewash with moisturizing, antibacterial and antioxidant effects, comprising the following components by mass percentage: Dendrobium nobile extract: 0.1-0.2%; Japanese cypress leaf extract: 0.1%-0.3%; Cooling agent: 0.001-0.005% Moisturizer: 0.1-3.0%; pH adjuster: 0.5-5.0%; Osmotic pressure regulator: 0.4-6.0%; The balance is purified water.

[0008] As a further preferred embodiment of the technical solution of the present invention, the cooling agent is menthol or borneol, or a mixture of both.

[0009] Furthermore, the moisturizing agent is a mixture of one or more of sodium hyaluronate, hydroxypropyl methylcellulose or blue lotus hydrosol.

[0010] Furthermore, the osmotic pressure regulator is a mixture of one or more of sodium chloride, potassium chloride or calcium chloride.

[0011] Furthermore, the pH adjuster is a mixture of one or more of dipotassium hydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, boric acid or borax.

[0012] The preparation method of the above-mentioned eyewash liquid with moisturizing, antibacterial and antioxidant functions comprises the following steps: (1) Weigh the Dendrobium nobile extract, Chamaecyparis obtusa leaf extract, cooling agent and moisturizing agent according to the formula, add part of purified water, and stir to mix evenly; (2) Add a pH regulator and an osmotic pressure regulator respectively to adjust the pH to 6.8-7.8 and the osmotic pressure to 260-340 mOsm / kg·H2O, and finally add the remaining purified water and stir to mix evenly; (3) Filter and sterilize the solution prepared in step (2), and take the filtrate and divide it into smaller packages to obtain the eyewash solution.

[0013] Furthermore, in step (1), the stirring and mixing time is 1-2 hours.

[0014] Furthermore, in step (2), the stirring and mixing time is 2-3 hours.

[0015] Furthermore, in step (3), the solution prepared in step (2) is filtered using a 0.22 μm microporous filter membrane; in order to ensure the sterilization effect, it is preferred to perform two filtrations.

[0016] By adopting the above technical solution, the beneficial effects achieved by the present invention are as follows: The Dendrobium nobile extract added in the present invention has alkaloids, polysaccharides, flavonoids, phenols, sesquiterpenes, coumarins and steroidal glycoside compounds as main components, has significant effects in scavenging free radicals, anti-oxidation, anti-aging and the like, can help eyes resist the damage of free radicals, reduce eye fatigue and dryness, and at the same time enhance the elasticity and firmness of eye skin.

[0017] The beneficial ingredients in Japanese cypress leaf extract include monoterpenes, monoterpene alcohols, sesquiterpenes, sesquiterpene alcohols, indole derivatives, hinokitiol, etc. As a natural antibacterial agent, Japanese cypress leaf extract has stable antibacterial components and has inhibitory effects on a variety of bacteria, including pneumococci, staphylococci, Escherichia coli, etc., showing broad-spectrum antibacterial properties. At the same time, as a natural antioxidant, it has a strong ability to clean up the activity of 1,1-diphenyl-2-picrylhydrazyl free radicals.

[0018] The polyphenols in the Dendrobium nobile extract and the hinokitiol in the Chamaecyparis obtusa leaf extract have synergistic effects in terms of antioxidant and antibacterial properties. Hinokitiol has antibacterial effects against a variety of bacteria, while polyphenols also have antioxidant and antibacterial properties. The combination of the two can enhance their antibacterial and antioxidant effects. Overall, the ingredients such as the Dendrobium nobile extract and the Chamaecyparis obtusa leaf extract in the eyewash work together to provide comprehensive care for the eyes. Their antibacterial, antioxidant and moisturizing properties make the eyewash an important tool for maintaining eye health and comfort. DETAILED DESCRIPTION

[0019] The components and properties of the eyewash solution of the present invention are described in detail below through specific examples.

[0020] In the following examples, the Dendrobium nobile extract was purchased from Guangzhou Meiyi Biotechnology Co., Ltd.; the Chamaecyparis obtusa leaf extract was purchased from Guangzhou Chennuo Biotechnology Co., Ltd.

[0021] Example 1 An eyewash with moisturizing, antibacterial and antioxidant effects, comprising: 0.1% of Dendrobium nobile extract, 0.1% of Chamaecyparis obtusa leaf extract, 0.002% of menthol, 0.2% of sodium hyaluronate, 1.0% of boric acid, 0.5% of borax, 0.9% of sodium chloride, and the balance of purified water.

[0022] Example 2 An eyewash with moisturizing, antibacterial and antioxidant effects, comprising: 0.2% of Dendrobium nobile extract, 0.2% of Chamaecyparis obtusa leaf extract, 0.003% of borneol, 0.4% of hydroxypropyl methylcellulose, 1.0% of boric acid, 0.5% of borax, 0.9% of sodium chloride, and the balance of purified water.

[0023] Example 3 An eyewash with moisturizing, antibacterial and antioxidant effects, comprising: 0.1% of Dendrobium nobile extract, 0.3% of Chamaecyparis obtusa leaf extract, 0.002% of menthol, 0.4% of hydroxypropyl methylcellulose, 1.0% of boric acid, 0.5% of borax, 0.9% of sodium chloride, and the balance of purified water.

[0024] Example 4 An eyewash with moisturizing, antibacterial and antioxidant effects, comprising: 0.2% of Dendrobium nobile extract, 0.1% of Chamaecyparis obtusifolia leaf extract, 0.005% of borneol, 0.2% of blue lotus hydrosol, 1.0% of boric acid, 0.5% of borax, 0.9% of sodium chloride, and the balance of purified water.

[0025] Example 5 An eyewash with moisturizing, antibacterial and antioxidant effects, comprising: 0.1% of Dendrobium nobile extract, 0.2% of Chamaecyparis obtusa leaf extract, 0.004% of menthol, 0.2% of sodium hyaluronate, 1.0% of boric acid, 0.5% of borax, 0.9% of sodium chloride, and the balance of purified water.

[0026] Comparative Example 1 The other ingredients and contents of Comparative Example 1 and Example 2 are the same, the only difference is that Comparative Example 2 does not add the antibacterial agent Japanese cypress leaf extract.

[0027] Comparative Example 2 The other ingredients and contents of Comparative Example 2 are the same as those of Example 4, except that the moisturizing antioxidant Dendrobium nobile extract is not added in Comparative Example 2.

[0028] Comparative Example 3 The other ingredients and contents of Comparative Example 3 are the same as those of Example 1, except that the Chamaecyparis obtusa leaf extract and the Dendrobium nobile extract are not added in Comparative Example 3.

[0029] The performance parameters of the eyewash solutions in the above embodiments and comparative examples are shown in Table 1.

[0030] The moisturizing effect, antibacterial effect and antioxidant effect of the eyewash were tested below.

[0031] 1. Eye effect test 40 people with chronic dry eyes were selected, and the eye wash solution was dropped into the eyes for eye entry test and wetness and moisturizing time test. At the same time, the eye wash solution was compared with the eye wash solution in comparative example 2, and the data were recorded, as shown in Table 2.

[0032] The results show that the eye wash liquid with Dendrobium nobile extract added into the eye shows that the moisture retention time of the eye wash liquid in Examples 1-5 is higher than that in Comparative Examples 2 and 3.

[0033] (II) Antibacterial effect test Experimental steps: 1. ① Take 10 ml of the eyewash solution in Examples 1-5 and Comparative Example 1 respectively for use; ② Culture medium: Use broth culture medium. ③ Activation of bacterial strains and preparation of bacterial suspension. Activation of bacterial strains: Pick the bacterial lawns of Staphylococcus aureus, Pneumococcus, and Escherichia coli respectively, streak them on the corresponding culture medium, and then place them in a constant temperature incubator for culture (Pneumococcus and Escherichia coli are cultured at 37°C for 24 hours, and Staphylococcus aureus is cultured at 35°C for 48 hours), activate them twice, and make a suspension with a live bacterial concentration of 10 6 cfu / mL of the bacteria for later use; 2. 1 mL of each eyewash solution obtained in Examples 1-5 and Comparative Example 1 was added to the above three bacterial suspensions, and the mixture was immersed at 37°C for 10 minutes. After the immersion, the number of live bacteria was detected by agar plate counting method. The data were recorded as shown in Table 3.

[0034] The above experimental data show that the bactericidal rates of Examples 1-5 against the three kinds of bacteria are significantly higher than those of Comparative Examples 1 and 3, indicating that the antibacterial effects of the eye wash solutions in Examples 1-5 are higher than those of Comparative Examples 1 and 3, and the antibacterial effect of Comparative Example 1 is significantly higher than that of Comparative Example 3, further indicating that the synergistic antibacterial effect of adding Japanese cypress leaf extract and Dendrobium nobile extract to the eye wash solution is more obvious.

[0035] (III) Antioxidant effect test Experimental steps: 1. Prepare 0.1mmol / L DPPH solution with anhydrous ethanol and store it away from light; 2. Add 2 mL of the test sample (Examples 1-5, Comparative Example 2) solution and 2 mL of DPPH solution into the same test tube, shake well, and let stand in the dark at room temperature for 30 min before measuring the absorbance A sample. At the same time, measure the absorbance Ao of 2 mL of DPPH solution mixed with 2 mL of anhydrous ethanol.

[0036] 3. Calculation formula: DPPH free radical scavenging rate (%) = (Ao-A sample) / Ao×100 Where Ao is the absorbance of DPPH at 517 nm without adding sample; A 样品 is the absorbance of DPPH added to the sample at 517 nm.

[0037] The data in Table 3 show that the free radical scavenging rate of the eyewash in Examples 1-5 is higher than that in Comparative Examples 2 and 3, and the free radical scavenging rate of Comparative Example 2 is significantly higher than that of Comparative Example 3, indicating that the combination of the Dendrobium nobile extract and the Chamaecyparis obtusa leaf extract has a strong ability to scavenge DPPH free radicals, further indicating that the combination of the two has a stronger antioxidant effect.

Claims

1. An eyewash with moisturizing, antibacterial and antioxidant effects, characterized in that: The eyewash solution comprises the following components in percentage by mass: Dendrobium nobile extract: 0.1-5%; Japanese cypress leaf extract: 0.1%-2%; Cooling agent: 0.001-0.005% Moisturizer: 0.1-3.0%； pH adjuster: 0.5-5.0%; Osmotic pressure regulator: 0.4-6.0%; The balance is purified water.

2. An eyewash liquid with moisturizing, antibacterial and antioxidant effects as claimed in claim 1, characterized in that: The cooling agent is menthol or borneol or a mixture of both.

3. An eyewash liquid with moisturizing, antibacterial and antioxidant effects as claimed in claim 1, characterized in that: The moisturizing agent is a mixture of one or more of sodium hyaluronate, hydroxypropyl methylcellulose or blue lotus hydrosol.

4. The eyewash liquid with moisturizing, antibacterial and antioxidant effects as claimed in claim 1, characterized in that: The osmotic pressure regulator is a mixture of one or more of sodium chloride, potassium chloride or calcium chloride.

5. The eyewash liquid with moisturizing, antibacterial and antioxidant effects as claimed in claim 1, characterized in that: The pH adjuster is a mixture of one or more of dipotassium hydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, boric acid or borax.

6. A method for preparing an eyewash liquid with moisturizing, antibacterial and antioxidant effects as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: (1) Weigh the Dendrobium nobile extract, Chamaecyparis obtusa leaf extract, cooling agent and moisturizing agent according to the formula, add part of purified water, and stir to mix evenly; (2) Add a pH regulator and an osmotic pressure regulator respectively to adjust the pH to 6.8-7.8 and the osmotic pressure to 260-340 mOsm / kg·H2O, and finally add the remaining purified water and stir to mix evenly; (3) Filter and sterilize the solution prepared in step (2), and take the filtrate and divide it into smaller packages to obtain the eyewash solution.

7. The method for preparing an eyewash liquid having moisturizing, antibacterial and antioxidant effects as claimed in claim 6, characterized in that: In step (1), the stirring and mixing time is 1-2 hours.

8. The method for preparing an eyewash liquid with moisturizing, antibacterial and antioxidant effects as claimed in claim 6 or 7, characterized in that: In step (2), the stirring and mixing time is 2-3 hours.

9. The method for preparing an eyewash liquid having moisturizing, antibacterial and antioxidant effects as claimed in claim 8, characterized in that: In step (3), the solution prepared in step (2) is filtered using a 0.22 μm microporous filter membrane.

10. The method for preparing an eyewash liquid with moisturizing, antibacterial and antioxidant effects as claimed in claim 9, characterized in that: In step (3), the solution prepared in step (2) is filtered twice using a 0.22 μm microporous filter membrane.

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