Bacillus subtilis and application thereof

A technology of Bacillus subtilis and microbial strains, applied in the field of microorganisms, can solve problems such as undeveloped and impact on rice quality, achieve strong antibacterial activity, and promote the effect of increasing crop production

Inactive Publication Date: 2012-12-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preventive overuse of chemical pesticides, especially at the booting stage, has a potential impact on rice quality
The use of biological control technology to control rice blast and bacterial blight has been studied at home and abroad, but no relatively mature products have been developed and applied to production

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the fermentation method of bacillus subtilis T500

[0023] The fermentation of Bacillus subtilis T500 comprises the steps:

[0024] 1) Strain activation: transfer Bacillus subtilis T500 to YPGA slant medium, and culture at 28°C for 48 hours;

[0025] 2) Seed culture: transfer the activated strain in step 1) into YPG culture solution, culture at 120 rpm, 28° C. for 48 hours.

[0026] 3), fermentation culture: in step 2), the content of bacteria in the seed tank reaches 10 10 When cfu / ml, put the fermentation hydraulic pressure in the seed tank into the fermenter for fermentation, the fermentation temperature is 28-32°C; the rotation speed: 80-150 rpm; Detect every 2 hours, observe the bacteria content and measure the pH of the fermentation broth. After 90% of the bacteria turned into spores, the detected bacteria content reached 2×10 10 cfu / ml out of the tank; after out of the tank, add benzoic acid preservative to the fermentation broth according to 2%...

Embodiment 2

[0030] Embodiment 2: Bacteriostasis of T500 on plant diseases

[0031] Determination of antibacterial activity against rice sheath blight: connect 9mm rice sheath blight bacteria cake to the center of the PDA medium plate, place 6 mm sterile filter paper sheets at three points, and absorb 10 μL of Bacillus subtilis fermentation broth on the filter paper cultured in an incubator at 28°C. The treatment without Bacillus subtilis was set as the control, and each treatment was repeated 3 times. When the plate was covered with pathogenic bacteria in the blank control, the diameter of the inhibition zone was measured and the inhibition rate was calculated. The method for measuring the antibacterial activity of Fusarium graminearum, Aspergillus flavus and Aspergillus niger is the same as that of Aspergillus oryzae. Determination of the antibacterial activity of rice smut fungus: Inoculate the rice smut fungus into PDA liquid medium, culture it on a shaker at 140 r / min, 28 ℃ for 5-7 d...

Embodiment 3

[0035] Embodiment 3, the compatibility of bacillus subtilis and tricyclazole

[0036] Under sterile conditions, Bacillus subtilis T500 was inserted into the NB culture medium and cultured at 28°C for 48 h on a shaker at 140 r / min. Weigh an appropriate amount of 20% tricyclazole, and prepare to contain tricyclazole concentrations of 100 μg / mL, 500 μg / mL, 1000 μg / mL, 2000 μg / mL, 3000 μg / mL, 4000 μg / mL, 5000 μg / mL potato plate and potato plate without tricyclazole, put filter paper at fixed points, and the filter paper contains 10 μL of cultured T500 fermentation broth, and use the filter paper in the potato plate without tricyclazole as a control , Observe and record the colony growth after culturing at 28°C for 2 days.

[0037] The results are shown in Table 3, indicating that the growth of Bacillus subtilis T500 was not affected on the medium with a tricyclazole concentration of 4000 μg / mL. When the medium contained 5000 μg / mL of tricyclazole, the colonies were smaller and t...

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Abstract

The invention relates to a Bacillus subtilis T-500. The Bacillus subtilis T-500 has been preserved in CGMCC (China General Microbiological Culture Collection), and the preservation number is CGMCC NO:6058. The Bacillus subtilis T-500 can be used as a biological pesticide for preventing and treating multiple plant diseases, and can also be used as a biological fertilizer for promoting the yield increase of crops. The Bacillus subtilis T-500 has higher antibacterial activity on Magnaporthe oryzae, Pellicularia sasakii, Xanthomonas oryzae, Ustilaginoidea virens, Xanthomonas oryzae pv. oryzicola, Fusarium graminearum, Aspergillus flavus, Aspergillus niger and other pathogenic bacteria, and has better prevention and control effects on diseases caused by the pathogenic bacteria. The Bacillus subtilis T-500 can prevent and treat rice leaf diseases when being diluted 200-500 times on the leaves of rice, can prevent and treat bakanae diseases of rice after seed soaking, and can prevent and treat soil-borne diseases of vegetables and the like after root irrigation.

Description

technical field [0001] The invention relates to a bacillus subtilis and application thereof, belonging to the field of microorganisms. Background technique [0002] At present, the control of plant diseases mainly relies on chemical pesticides, which have made great contributions to the safe production of agricultural products, but the widespread use of chemical pesticides and the increase in the amount of pesticides have caused a series of problems, such as pathogenic bacteria becoming resistant to pesticides, and pesticide residues in agricultural products exceeding the standard , farmland ecological environment pollution and other issues. These problems increase the cost of farmland production, and at the same time, the agricultural products with excellent quality are affected by the excessive use of pesticides. New control technologies and disease control methods have become the direction of exploration for plant protection scientists, and the use of Bacillus subtilis t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00C05F11/08C12R1/125
Inventor 刘永锋陈志谊于俊杰刘邮洲聂亚锋
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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