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Streptomyces katrae NB20, as well as culture method and application thereof

A technology of cattrazia and zipper mold, applied in the field of streptomyces cattrazia NB20 and its cultivation, to achieve good environmental compatibility, not easy to produce resistance, and good control effect

Inactive Publication Date: 2012-05-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the control of plant diseases by Streptomyces catatrae

Method used

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  • Streptomyces katrae NB20, as well as culture method and application thereof
  • Streptomyces katrae NB20, as well as culture method and application thereof
  • Streptomyces katrae NB20, as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Screening and identification of Streptomyces katella NB20

[0017] 1. Screening of strains

[0018] (1) Collection of soil samples

[0019] In this experiment, soil samples were collected from the farm orchard of South China Agricultural University. According to the topography of the collection area, 5 points were used for sampling. The sampling depth was 15 cm below the cultivated layer or the ground surface. The amount was 200-250 g. The 5 samples collected at each location were uniform Mix, put it into a sterile sealed polyethylene bag, and bring it back to the laboratory for immediate separation.

[0020] (2) Preparation of sample suspension

[0021] Add the soil sample to sterilized water and shake vigorously. Sample: water = 1:9 (mass to volume ratio), such as: 10 g of soil is added to a triangular flask containing 90 mL of sterilized water to obtain a suspension concentration of 10 -1 , Prepare 5 test tubes containing 9 mL sterile water for each sample acco...

Embodiment 2

[0047] Example 2 Inhibition of Streptomyces katella NB20 on plant pathogens

[0048] 1. The inhibitory effect of Streptomyces katella NB20 on a variety of plant pathogens

[0049] (1) The pathogenic fungi and bacterial strains tested

[0050] The plant pathogenic fungi and pathogenic bacteria used in this experiment are all strains preserved in our laboratory.

[0051] (2) Inhibition of pathogenic fungi

[0052] Use a sterile inoculation needle to inoculate the NB20 strain of Streptomyces katella on one side of the PDA medium plate, and at the same time, the banana anthracnose C. musae ), Magnaporthe grisea ( P. oryzae ), Fusarium graminearum ( G. zeae ), Banana Fusarium Wilt ( F. oxysporum f. sp. cubense ), lychee frosty phytophthora ( P. litchii ), sugarcane black rot fungus ( C. adiposum ) And tomato leaf mold ( C. fulvum ) And other pathogenic fungi were inoculated on the other side of the PDA medium plate for confrontation culture (that is, a pathogen and Streptomyces cat...

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Abstract

The invention discloses streptomyces katrae NB 20, as well as a culture method and an application thereof. The strain with a preservation number of CGMCC No.5382 is preserved in China General Microbiological Culture Collection Center on October 24, 2011. Researches prove that: the strain has strong inhibition effects to colletotrichum musae, pyricularia grisea, fusarium graminearum, fusarium oxysporum f.sp.cubense, peronophythora litchi, ustilago scitaminea, fulvia fulva, xanthomonas oryzae and xanthomonas oryzae pv.oryzicola, is a broad-spectrum antagonistic strain, can be used for prevention and control of various plant diseases, has a particular good prevention and control effect to the colletotrichum musae, has the advantages of harmlessness to human and livestock, greenness and safety, good environment compatibility, difficult resistance generation and the like, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection (microbial pesticides), and in particular relates to Streptomyces catatrae NB20 and its cultivation method and application. Background technique [0002] Plant diseases caused by plant pathogenic bacteria (including fungi, bacteria, viruses, etc.) have caused significant economic losses to agricultural production. Banana is a world-famous tropical fruit and one of the four famous fruits in South China. The banana industry plays an important role in the agricultural and social development of South China. Post-harvest banana fruit rot has always been one of the important factors restricting the development of banana industry in my country, and the rot loss is as high as 15% to 30%, while the banana anthracnose bacteria ( Colletotrichum musae ) caused by anthracnose (anthracnose) is one of the important causes of banana fruit rot, which seriously affects the northward transportation and export of ban...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/465
Inventor 杨媚舒灿伟周而勋高艳丽张德涛范家平
Owner SOUTH CHINA AGRI UNIV
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