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Bidirectional burkholderia as well as culture method and application thereof

A technology of Holderia and culture, applied in the field of bidirectional Burkholderia and its cultivation, can solve the problems of lack of biological control drugs, large side effects of chemical agents for plant disease control, etc.

Inactive Publication Date: 2014-01-29
西安西诺农化有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method that can inhibit the growth of plant diseases including banana anthracnose bacteria and rice sheath blight bacteria in the prior art, in view of the defects of large side effects and lack of biological control drugs caused by various plant pathogenic fungi and bacteria in the prior art. Broad-spectrum biocontrol strains for a variety of plant pathogens

Method used

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  • Bidirectional burkholderia as well as culture method and application thereof
  • Bidirectional burkholderia as well as culture method and application thereof
  • Bidirectional burkholderia as well as culture method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Screening and identification of embodiment 1 bidirectional Burkholderia PPMMD

[0019] 1. Screening of bacterial strains

[0020] (1) Collection of soil samples

[0021] In this experiment, soil samples were collected from the farm orchard of South China Agricultural University. According to the topography of the collection site, 5 points were used for sampling. The sampling depth was 15cm below the plow layer or the surface, and the sampling volume was 200-250g. The 5 samples collected at each location were evenly mixed. , put into a sterilized sealed polyethylene bag, bring it back to the laboratory and separate it immediately.

[0022] (2) Preparation of sample suspension

[0023] Add the soil sample into sterile water and shake vigorously. Sample: water = 1:9 (mass to volume ratio), such as: 10g of soil is added to a conical flask filled with 90mL of sterilized water to obtain a suspension concentration of 10 -1 . Prepare 5 test tubes containing 9 mL of sterili...

Embodiment 2

[0038] The inhibitory action of embodiment 2 bidirectional Burkholderia PPMMD to plant pathogenic bacteria

[0039] 1. Control effect of bidirectional Burkholderia PPMMD on banana fruit anthracnose

[0040] (1) Tested banana anthracnose strains

[0041] The banana anthracnose bacterium (C.musae) used for inoculation was isolated and purified from the diseased banana fruit, and was preserved by our laboratory.

[0042] (2) Control effect of Burkholderia bidirectional PPMMD on banana fruit anthracnose

[0043] Inoculate 1 mL of 2-day-old Burkholderia bidirectional PPMMD strain seeds per 100 mL of LB medium (peptone: 10 g / L, yeast extract powder: 5 g / L, etc., NaCl: 10 g / L, pH: 7.0-7.2) Proportional seed inoculation and expansion culture, the culture conditions are: rotation speed 200r / min, 25-30°C culture for 20-24 hours, after centrifugation, collect the supernatant, filter with a bacterial filter to obtain a sterile filtrate. Select seven or eight mature bananas, disinfect t...

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Abstract

The invention relates to bidirectional burkholderia as well as a culture method and an application thereof and belongs to the field of plant protection (microbial pesticide). The preservation number of the bidirectional burkholderia is CCTCC NO: M2013145. The culture method of the bidirectional burkholderia comprises the following steps: adding 1ml of 2-day-aged seed bacterial strain to an LB (Lysogeny broth) culture medium with the liquid loading of 50ml under a fermentation condition, and culturing for 20-24 hours at the temperature of 25-30 DEG C at the rate of 200r / min. The bidirectional burkholderia has a remarkable inhibition effect on rice sheath blight disease, rice blast, colletotrichum musae, oxysporum, fusarium asiaticum, peronophythora litchi, sugarcane alternaria tenuis, cladosporium fulvum, xanthomonas oryzae, bacterial leaf steak and the like, can be used for the biological prevention and control of plant pathogenic bacteria, has the advantages of low toxicity, easy degradation, environmental friendliness and the like and has a bright application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection (microbial pesticides), and in particular relates to a bidirectional Burkholderia and its cultivation method and application. Background technique [0002] Plant diseases caused by plant pathogenic bacteria (including fungi, bacteria, viruses, etc.) have caused significant economic losses to agricultural production. At present, the main method of preventing and treating important plant diseases such as rice and bananas is to use fungicides. However, the extensive use of fungicides has the risk of increasing the resistance of pathogenic bacteria, causing serious pollution to the environment, and posing a threat to the health of humans and animals. Biological control is a new way to prevent and control plant diseases that is environmentally friendly. [0003] There are not only pathogenic bacteria around plants, but also a large number of non-pathogenic bacteria, some of which are antagonistic micro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P1/00C12R1/01
Inventor 杨忠绪张小荣
Owner 西安西诺农化有限责任公司
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