Bacillus velezensis CSQXDZ26 strain and application thereof

A technology of Bacillus and Veles, which is applied in the field of microorganisms, can solve the problems that plant pathogenic fungi do not show bacteriostasis and inhibition, and achieve good biocontrol effects, good inhibitory effects, and good application prospects

Active Publication Date: 2020-07-31
ZHEJIANG NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it does not exhibit bacteriostatic activity against phytopathogenic fungi (e.g., X. poplar, Fusarium rubella, X. tomato, X. tobacco)
[0013] The above-mentioned Bacillus Veles only has antibacterial activity on bacterial plant diseases, or only on fungal plant diseases, and does not reflect common food spoilage bacteria ( For example, Bacillus subtilis, Proteus, Staphylococcus aureus, etc.) have inhibitory effect

Method used

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  • Bacillus velezensis CSQXDZ26 strain and application thereof
  • Bacillus velezensis CSQXDZ26 strain and application thereof
  • Bacillus velezensis CSQXDZ26 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, the isolation and identification of Bacillus velezensis (Bacillus velezensis) CSQXDZ26 bacterial strain

[0038] (1) culture medium

[0039] Beef extract peptone solid medium: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, distilled water 1L, pH7.0-7.2;

[0040] PDA medium: 200g potato, 20g glucose, 20g agar, 1L distilled water, natural pH;

[0041] Improved PDA medium: 300g potato, 15g sucrose, Ca(NO 3 ) 2 4H 2 O 0.5g, Na 2 HPO 4 12H 2 O 2g, tryptone 5g, agar 20g, distilled water 1L, pH natural.

[0042] PMT medium liquid medium: potatoes 200g, maltose 20g, peptone 10g, Na 2 HPO 4 1.5g, (NH 4 ) 2 SO 4 5g, 1L distilled water, natural pH.

[0043] (2) Sample collection and processing

[0044] The samples were collected from the fifth-instar fresh silkworm excrement of Guican No. 2, Wuping Township, Jingxi City, Guangxi (23°14’N, 106°22’E), dried in an oven at 105°C for 90 minutes, and set aside.

[0045] (3) Preparation of silkworm excreme...

Embodiment 2

[0060] Embodiment 2, the preparation of Bacillus velezensis (Bacillus velezensis) CSQXDZ26 bacterial strain fermented liquid and its stability determination

[0061] (1) Preparation of fermentation broth

[0062] Take the Bacillus Velez CSQXDZ26 strain stored in the refrigerator at –80°C and spread it on the plate of beef extract peptone solid medium, culture it at 30°C for 24 hours, and then pick a single colony and inoculate it into a 250mL shaker containing 50mL PMT liquid medium. Place in the flask and culture in a shaker at 30°C and 200r / min for 12h as the seed liquid; take 4mL of seed liquid into a 1000mL Erlenmeyer flask filled with 400mL of PMT culture solution, and culture in a shaker at 30°C and 200r / min After 72 hours, the cultured product was centrifuged at 12,000 r / min for 15 minutes, and the supernatant was collected, which was the fermentation broth.

[0063] (2) Determination of the stability of fermented liquid antagonizing rice bacterial spot bacterium:

[...

Embodiment 3

[0072] Embodiment 3, Bacillus velezensis (Bacillus velezensis) CSQXDZ26 bacterial strain fermented liquid is to the antagonism experiment of 9 kinds of plant pathogenic bacteria

[0073] (1) Plant pathogenic bacteria for testing:

[0074] Xanthomonas oryzae pv.Oryzae, Xanthomonas oryzae pv.oryzicola, Xanthomonas axonopodispv.glycines, Dothiorella gregaria, Gibberella wheat Fusarium graminearum, Fusarium oxysporum.sp.cucumebrium Owen, Fusarium oxysporum f.sp.Momordicae, Alternaria solani, Alternaria alternate.

[0075] (2) culture medium

[0076] Beef extract peptone medium, PDA medium, modified PDA medium and PMT medium.

[0077] (3) method

[0078] The method for obtaining the fermentation broth refers to Example 2. The rice bacterial blight, the rice bacterial streak and the soybean spot were cultured and activated on the improved PDA medium for 3 days, and the concentration was 10 8cfu / mL bacterial solution, for later use; cultivate and activate the other 6 kinds of ph...

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Abstract

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

Description

technical field [0001] The invention belongs to the technical field of microbes, and specifically relates to a Bacillus velezensis CSQXDZ26 strain which has significant antagonistic effects on various plant pathogenic bacteria and food spoilage bacteria and an application thereof. Background technique [0002] There are many kinds of crop diseases and great impacts, which often cause heavy losses to agricultural production and directly restrict the sustainable development of agriculture. The application of pesticides is an important means to effectively control crop diseases, but chemical pesticides can cause environmental pollution, ecological imbalance, drug resistance and food safety, and biological control has good environmental compatibility, less residues, high selectivity, and The advantages of human and animal safety and less resistance to pesticides have gradually attracted people's attention, and they have become the first choice to replace chemical pesticides. At...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
CPCC12N1/20C12R2001/07C12N1/205
Inventor 张萍华陈宝婷苏盼徐俏兰王明会张艳军丁林贤王海芬
Owner ZHEJIANG NORMAL UNIVERSITY
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