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Burkholderia cepacia SD7 and culturing method and application thereof

A technology of Burkholderia and onion, applied in the field of Burkholderia cepacia SD7 and its cultivation, can solve the problems of large side effects of chemical agents for plant disease prevention and control, lack of biological control drugs, etc., which is not easy to achieve. The effect of producing resistance, strong inhibitory effect, and broad application prospects

Inactive Publication Date: 2012-07-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method that can inhibit the growth of plant diseases including banana anthracnose bacteria and rice sheath blight bacteria in the prior art, in view of the defects of large side effects and lack of biological control drugs caused by various plant pathogenic fungi and bacteria in the prior art. Broad-spectrum biocontrol strains for a variety of plant pathogens

Method used

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  • Burkholderia cepacia SD7 and culturing method and application thereof
  • Burkholderia cepacia SD7 and culturing method and application thereof
  • Burkholderia cepacia SD7 and culturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Screening and Identification of Burkholderia cepacia SD7

[0020] 1. Screening of bacterial strains

[0021] (1) Collection of soil samples

[0022] In this experiment, soil samples were collected from the farm orchard of South China Agricultural University. According to the topography of the collection site, five points were used for sampling. The sampling depth was 15 cm below the plow layer or the surface, and the sampling volume was 200-250 g. The five samples collected at each site were evenly distributed. Mix them, put them into sterilized sealed polyethylene bags, and bring them back to the laboratory for immediate separation.

[0023] (2) Preparation of sample suspension

[0024] Add the soil sample into sterile water and shake vigorously. Sample: water = 1:9 (mass to volume ratio), such as: 10 g of soil is added to a conical flask filled with 90 mL of sterilized water to obtain a suspension concentration of 10 -1 . Prepare 5 test tubes co...

Embodiment 2

[0040] Example 2 Inhibitory effect of Burkholderia cepacia SD7 on plant pathogenic bacteria

[0041] 1. Control effect of Burkholderia cepacia SD7 on banana fruit anthracnose

[0042] (1) Tested banana anthracnose strains

[0043] Banana anthracnose bacteria used for inoculation ( C. musae ) was isolated and purified from the diseased banana fruit, and was preserved by our laboratory.

[0044] (2) Control effect of Burkholderia cepacia SD7 on banana fruit anthracnose

[0045] For every 100 mL of 2-day-old seeds of Burkholderia cepacia SD7 strain (yeast extract powder 5 g, tryptone 10 g, NaCl 10 g, agar powder 15-18 g, water 1000 mL, pH 7.0- 7.2) Inoculate 1 mL of seeds at a ratio of inoculation to expand the culture. The culture conditions are: rotation speed 180 r / min, 25°C, after 3 days of culture, collect the supernatant after centrifugation, and filter with a bacterial filter to obtain a sterile filtrate. Select seven or eight mature bananas, disinfect the surface w...

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Abstract

The invention discloses burkholderia cepacia SD7 and a culturing method and application thereof. The bacterial strain is preserved in China General Microbiological Culture Collection Center on October 24th, 2001, and the preservation number is CGMCC No.5384. Through research, the bacterial strain has strong inhibiting effect on rhizoctonia solani, collectotrichum musae, pyricularia grisea, gibberella zeae, fusarium oxysporum f. sp. cubense, peronophythora litchi, ustiago scitamimea syd, fulvia fulva, xanthomonas oryzaepv.oryzae, xanthomonas oryzae pv. Oryzicola, and the like, is broad-spectrum antagonistic bacteria, can be used for preventing and treating a plurality of plant diseases, particularly has better preventing and treating effect for banana fruit anthracnose and rice sheath blight, has the advantages of harmlessness to person and animals, environmental protection and safety, good environmental compatibility, and difficulty in becoming resistant, is good for business development, and has broad application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection (microbial pesticides), and in particular relates to Burkholderia cepacia SD7 and its cultivation method and application. Background technique [0002] Plant diseases caused by plant pathogenic bacteria (including fungi, bacteria, viruses, etc.) have caused significant economic losses to agricultural production. At present, the main method of preventing and treating important plant diseases such as rice and bananas is to use fungicides. Extensive use of fungicides will increase the risk of pathogenic bacteria resistance, and will cause serious pollution to the environment and pose a threat to human and animal health. Biological control is a new way to prevent and control plant diseases which is friendly to the environment. [0003] There are not only pathogenic bacteria around plants, but also a large number of non-pathogenic bacteria, some of which are antagonistic microorganisms of pathogenic b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/01
Inventor 周而勋舒灿伟杨媚高艳丽张德涛彭正凯
Owner SOUTH CHINA AGRI UNIV
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