33 results about "Xanthomonas citri" patented technology

The invention belongs to the technical field of genetic engineering, and particularly relates to a citrus canker pathogen mediated citrus leaf transient expression method which comprises the following steps: preparing a citrus canker pathogen infection solution which is xanthomonas citrus subspecies; infecting the citrus by using the citrus canker pathogen infection liquid, and culturing to obtain citrus infected by the citrus canker pathogen; preparing an agrobacterium infection solution containing a target gene; and inoculating the citrus infected by the citrus canker pathogen with an agrobacterium infection solution containing a target gene, and carrying out transient expression of the target gene. The transient expression method provided by the invention can realize transient and efficient expression of the target gene in leaves of different citrus varieties. The transient expression method disclosed by the invention can be used for researching gene functions, protein interaction, promoter activity analysis and the like; and the method can also be used for evaluating the citrus canker resistance gene function.

The present invention discloses the application of isoquinoline-3-carboxylic acid in preventing and controlling plant bacterial diseases. The isoquinoline-3-carboxylic acid provided by the present invention has an obvious inhibitory effect on plant bacterial diseases, and the plant bacterial diseases are Ralstonia solanacearum, Acidovorax avenae subsp. Citrulli, Pseudomonas syringae pv. actinidiae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Alternaria brassicae, Xanthomonas citri subsp. citri, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas fragariae, Pseudomonas syringae pv. lachrymans, Dickeya dadantii, Xanthomonas axonopodis pv. mangiferaeindicae. The isoquinoline-3-carboxylic acid of the present invention has protective and therapeutic effects on Acidovorax avenae subsp. Citrulli.

The invention discloses bacillus altitudinis and an application of the bacillus altitudinis in prevention and treatment of plant diseases. The bacillus altitudinis CDJ1 is preserved in Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65289. The bacillus altitudinis CDJ1 disclosed by the invention can be used for effectively resisting pectobacterium taro, and in the formula, the bacillus altitudinis CDJ1 can be used for effectively resisting Desia Dabesii, Xanthomonas citrus pathogenic variants, Xanthomonas citrus mango pathogenic variants, Xanthomonas campestris, Xanthomonas sp., Xanthomonas sp., Xanthomonas sp. And Xanthomonas arborescens plum pathogenic variants, so that the bacillus altitudinis CDJ1 can be used for effectively resisting pectobacterium taro and the bacillus altitudinis CDJ1 can be used for effectively resisting pectobacterium taro. The composition has a good prevention and treatment effect on various plant diseases. When the bacillus altitudinis CDJ1 is prepared into a microbial agent, the use of chemical pesticides can be reduced, and the bacillus altitudinis CDJ1 has a wide application prospect in the field of agricultural biocontrol.

The invention discloses a 1, 8-disubstituted tryptanthrin derivative and application of the 1, 8-disubstituted tryptanthrin derivative to prevention and treatment of phytopathogen and anti-tumor activity, the general formula of the derivative is shown in (I), and the test result of the compound shown in the general formula on the bacterial activity of phytopathogen shows that the compound has a good antibacterial effect on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and the like, and can be used for preventing and treating the phytopathogen. The method can be used for developing novel agricultural bactericides. Meanwhile, results of the compound shown in the general formula on tumor cells show that the compound has good in-vitro anti-tumor activity on human chronic myelogenous leukemia cells K562, human lung cancer cell lines A549, human liver cancer cells HepG2, human prostate cancer cells PC3 and the like, and can be used for researching novel anti-tumor drugs. # imgabs0 #

The present invention belongs to the technical field of plant disease prevention and control, and specifically relates to an agricultural composition of Paenibacillus polymyxa and a gallnut extract and its application. The present invention first discovered that a gallnut extract has an inhibitory effect on the growth of four different pathogens: Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The invention also discovered that the gallnut extract has a synergistic effect on Paenibacillus polymyxa. The invention also provides an agricultural composition composed of a gallnut extract and Paenibacillus polymyxa. The agricultural composition has an inhibitory effect on the growth of Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The agricultural composition can be applied to the field of plant disease prevention and control.

The invention discloses xanthomonas citri subsp.citri phage and application thereof, and relates to the technical field of biology. The xanthomonas citrus subspecies phage is preserved in the China Center for Type Culture Collection on July 4, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20241486. The xanthomonas citrus subsp.citri bacteriophage can specifically inactivate the xanthomonas citrus subsp.citri, mass proliferation can be completed only with a small amount of initial bacteriophage, and a high-quality bacteriophage strain source is provided for industrial production of bacteriophage bactericides; technicists in the field can prepare various products applied to the aspects of detection, disinfection, plant protection and the like from the xanthomonas citrus subsp.citri phage provided by the invention according to records and common knowledge in the field by technicians in the field, and the products are industrially applied to the xanthomonas citrus subsp.citri phage.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The present invention provides a new bacteriophage with SEQ ID No.: 1 against Xanthomonas campestris and / or Xanthomonas citri infections and use of same for treating and / or preventing a Xanthomonas campestris and / or Xanthomonas citri infection in a crop.

The invention discloses a purine nucleoside compound containing a cinnamic acid structure as well as a preparation method and application of the purine nucleoside compound, and a series of purine nucleoside compounds containing the cinnamic acid structure are synthesized by taking a purine nucleoside compound as a basis and introducing a piperazine-containing cinnamic acid fragment into a system. The compound has a good inhibition effect on plant pathogenic microorganisms, and especially has a good inhibition effect on plant pathogenic viruses such as tobacco mosaic viruses. Wherein the activity data of a part of the compounds is superior to that of a commercial drug ribavirin, the activity of the compounds is equivalent to that of ningnanmycin, particularly, the compound 14 has excellent treatment activity, and the EC50 of the compound 14 is 274.25 mu g / mL. The compound has good antibacterial activity on Xanthomonas oryzae pv. Oryzae, when the concentration is 50 [mu] g / mL, the inhibitory activities of the compound 7, the compound 20 and the compound 21 on Xanthomonas oryzae pv. Oryzae are 36.2%, 40.3% and 35.6% respectively and are superior to 32.6% of a control agent thiediazole copper, when the concentration is 50 [mu] g / mL or below, the inhibitory effects of the compound 1, the compound 3, the compound 23 and the compound 24 on Xanthomonas citri are 21.3%, 20.4%, 27.8% and 22.6% respectively, and the compound 7, the compound 20 and the compound 21 on Xanthomonas oryzae pv. Oryzae are superior to that of the control agent thiediazole copper. And the results are better than that of a control agent thiediazole copper by 18.6%.

The invention discloses a preparation method and application of an indole C2-position derivative containing a 1, 3, 4-oxadiazole and thioether structure. The compound can be used for effectively preventing and treating bacterial diseases such as kiwi fruit canker pathogen, citrus canker pathogen and rice bacterial blight pathogen. Wherein the EC50 value of the compound 2-((2-fluorophenyl) sulfo)-5-(1H-indol-2-yl)-1, 3, 4-oxadiazole on pathogenic bacteria of the kiwifruit canker is 27.35 mu g / mL, and the prevention and treatment effect is the best; the EC50 values of the compound 2-(1H-indole-2-yl)-5-((4-trifluoromethoxy-phenyl) sulfo)-1, 3, 4-oxadiazole on pathogenic bacteria of citrus canker and pathogenic bacteria of rice bacterial blight are 32.94 mu g / mL and 49.52 mu g / mL respectively, and the control effect is the most ideal. The pesticide composition is obviously superior to control pesticides such as bismerthiazol and thiediazole copper. The indole C2-site derivative containing the 1, 3, 4-oxadiazole and thioether structure can be applied to prevention and treatment of bacterial diseases of crops, and is simple in structure, convenient in preparation process, low in production cost and wide in application prospect.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

The structural formula of the thymol-containing quaternary ammonium salt derivative is shown as (1), wherein R1 is 2-isopropyl-5-methylphenyl, ethyl or a benzene ring, R2 is 2-chloro-5-methylthiophene, 3-methyl-2-methoxycarbonyl furan, 4-methylnaphthalene and differently substituted benzyl, X-is Cl-and Br-, and n is equal to 3, 5, 7, 8 and 9. The preparation method of the drug-loaded antibacterial liposome comprises the step of jointly assembling hydrogenated soybean phosphatidylcholine, cholesterol hemisuccinic acid monoester, phosphatidyl ethanolamine and a target compound thymol-containing quaternary ammonium salt derivative into the drug-loaded antibacterial liposome. Part of the compounds have excellent antibacterial activity on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri and Xanthomonas syringae pv. Kiwifruit. Meanwhile, the compound also has excellent biological activity on six fungi such as fusarium oxysporum and TMV viruses. A field test on Xoo shows that the protection effect of the drug-loaded antibacterial liposome is improved by 1.94 times, the treatment effect is improved by 1.56 times, and the drug-loaded antibacterial liposome shows remarkable pH-triggered release characteristics and colloidal stability. (1)

The invention discloses a cinchona alkaloid derivative for preventing and treating agricultural diseases as well as a preparation method and application thereof. The cinchona alkaloid derivative comprises a compound with a structural formula as shown in formula I, or pharmaceutically acceptable salt, solvate and tautomer thereof. It is found that the cinchona alkaloid derivative has an excellent inhibition effect on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri, has great development value in the aspect of treating plant pathogenic bacteria, and provides more choices for agricultural disease control.

The invention provides a pleuromutilin derivative containing a thioacetate side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I

The invention provides a pleuromutilin derivative containing an amino side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The application discloses a method, reagent and application for identifying or tracing citrus canker bacteria. The method comprises the following steps: obtaining seven gene sequences of the to-be-tested citrus canker bacteria, performing multi-locus sequence typing analysis on the seven gene sequences, determining alleles and numbering, concatenating the allele numbers of the seven genes to serve as a sequence type, comparing the sequence type with a reference strain sequence type, and obtaining tracing information of the to-be-tested strain; the reference strain is a citrus canker bacteria with known tracing information; the seven genes are XAC_RS07980, XAC_RS21550, copB, egl, fliL, hrpA and pliY. The seven housekeeping genes suitable for multi-locus sequence typing of the citrus canker bacteria are used for tracing detection, different geographical source strains can be distinguished at high resolution, and the method has important significance for strain regional research, evolution, transmission, disease prevalence and prevention and treatment. The method can be used for identifying and tracing the citrus canker bacteria.

The invention discloses a 2-fluoro-3-aliphatic amine tryptanthrin derivative and application of the compound to prevention and treatment of phytopathogen and anti-tumor activity, the general formula of the derivative is as shown in (I), and the test result of the compound as shown in the general formula to the bacterial activity of phytopathogen shows that the compound can be used for preparing the 2-fluoro-3-aliphatic amine tryptanthrin derivative. The compound has a good antibacterial effect on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and the like, and can be used for developing novel agricultural bactericides. Meanwhile, results of the compound shown in the general formula on tumor cells show that the compound has good in-vitro anti-tumor activity on human chronic myelogenous leukemia cells K562, human lung cancer cell lines A549, human liver cancer cells HepG2, human prostate cancer cells PC3 and the like, and can be used for researching novel anti-tumor drugs. # imgabs0 #

The invention provides a pleuromutilin derivative containing a thioether side chain and application of the pleuromutilin derivative in prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Pseudomonas solanacearum pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia pv. Lanceolatifolia. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The invention relates to the technical field of plant disease prevention and control, and particularly discloses a grapefruit disease prevention and seedling raising method, specifically, according to the prevention and control method provided by the invention, a bactericide with an effective component of an ethanol extract of branches and leaves of kukuhua catalpa is used, and the bactericide is sprayed on grapefruit tree seedlings. The Yunnan catalpa bungei extract can effectively prevent and control pomelo tree canker caused by infection of pathogenic bacteria Xanthomonas citri subsp.citri (Xcc), when the effective concentration is 500 mg / L, the relative control effect reaches up to 83.52%, a green and sustainable way is provided for prevention and control of pomelo tree canker, and occurrence of the pomelo tree canker is expected to be relieved.

The invention discloses application of 2, 6-dicarboxypyridine in inhibition of growth of plant pathogenic bacteria, and belongs to the technical field of biological agriculture. The invention firstly discovers that the 2, 6-dicarboxyl pyridine can be used for inhibiting the growth of Xanthomonas plant pathogenic bacteria, especially Xanthomonas carpesium xanthomonas soybean pathogenic variants (Xanthomonas citrulli pv.glycines, Xcg), Xanthomonas oryzae pv.oryzae, Xoo, Xanthomonas oryzae pv.oryzae, Xoc, Xanthomonas campestris pv.oryzae pv.oryzae, Xanthomonas campestris pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae pv.oryzae Xcc), and can be used for preparing pesticides for preventing and treating plant pathogenic bacteria.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.

The invention discloses a citrus canker phage SAC and application thereof.The citrus canker phage SAC is preserved in the China General Microbiological Culture Collection Center on January 20, 2025, and the preservation number is CGMCC No.46373. The phage can effectively split the pathogenic bacteria, namely citrus xanthomonas subsp. Citricola, of citrus canker, so that the citrus canker phage SAC has the advantages of being capable of effectively splitting the pathogenic bacteria, namely citrus xanthomonas subsp. Citricola, of the citrus canker, and capable of effectively splitting the pathogenic bacteria, namely citrus subsp. Citricola, of the citrus canker. An agricultural bactericide, a disinfectant or a cleaning agent prepared based on the bacteriophage can effectively prevent and treat citrus canker and reduce pollution of pathogenic bacteria in an orchard environment. The bacteriophage and the biological prevention and treatment means provided by the invention have good application prospects, can reduce the disease incidence, reduce the yield loss, avoid the use of chemical pesticides, and are beneficial to protecting the ecological environment and the health of consumers.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

The invention discloses an imidazo pyrimidone-containing derivative and a preparation method and application thereof, and a series of imidazo pyrimidone-containing skeleton derivatives are synthesized by taking an imidazo pyrimidone-containing skeleton as a pilot structure and grafting different pharmacophores on the basis of the pilot structure. In-vitro tests of the compounds show that the compounds have effects on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, Xanthomonas oryzae pv. Oryzae and Xanthomonas syringae pv. Actinidia; the compound has an excellent inhibition effect on plant pathogenic fungi such as rhizoctonia solani, colletotrichum chinense, alternaria solani and the like. Especially, the EC50 of most of the halogen-containing compounds to Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri is within 10 [mu] g / mL, especially the EC50 of the compound 14 to Xanthomonas oryzae pv. Oryzae is 3.86 [mu] g / mL, the EC50 of the compound 14 to Xanthomonas citri pv. Citri is 4.04 [mu] g / mL, and the halogen-containing compounds have good antibacterial activity.

The application discloses a quinina derivative for preventing and treating agricultural diseases, a preparation method and application thereof, and relates to a compound with a structural formula as shown in formula I, or a pharmaceutically acceptable salt, a solvate and a tautomer thereof. It is found that the quinina derivative has excellent inhibitory effect on Xanthomonas oryzae and Xanthomonas citri, has great development value in treating plant pathogenic bacteria, and provides more choices for preventing and treating agricultural diseases.

The invention provides a pleuromutilin derivative containing an ester side chain or a silicon-oxygen side chain and application of the pleuromutilin derivative to prevention and treatment of plant diseases. The pleuromutilin derivative is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The pleuromutilin derivative disclosed by the invention shows excellent bacteriostatic activity on phytopathogens such as pathogenic bacteria, and particularly shows a remarkable inhibition effect on xanthomonas pathogenic bacteria and Releiella pathogenic bacteria, and particularly on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis pv. Citrullis. The pleuromutilin derivative is low in preparation difficulty, and raw materials are cheap and easy to obtain. Therefore, the compound has further development value and is expected to be developed into a novel agricultural bactericide. Formula I.

The present invention belongs to the technical field of the application of nanomaterials, and particularly relates to the application of Ag-SiO2 nanocomposites in the prevention and control of citrus pests and diseases. The technical problem to be solved by the present invention is to provide a new option for the prevention and control of citrus pests and diseases. In order to solve the technical problem, the present invention optimizes the synthesis method of Ag-SiO2 nanocomposites; and through experiments, it is verified that the Ag-SiO2 nanocomposites have high toxicity to Diaphorina citri and Xanthomonas citri subsp. citri, can be used as an effective insecticide for Diaphorina citri, and can also be used as a new type of fungicide for the prevention and control of citrus canker, providing a new option for the prevention and control of citrus pests and diseases.

The invention discloses a novel thiourea substance, which is characterized in that the structural formula of the novel thiourea substance is as follows: # imgabs0 #, wherein R phenyl, substituted phenyl and heterocyclic ring. A biological activity determination result shows that the acylthiourea compound containing the purine part has good inhibitory activity on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwifruit. The compound C19 has high activity, especially has medium to excellent inhibitory activity on rice bacterial leaf streak, the activity of the compound C19 is the best, and the inhibitory activity on the rice bacterial leaf streak reaches 92.0% and is far higher than that of control agents thiediazole copper and zinc thiazole.

The present invention discloses the application of the citrus CsGRAS9 gene in improving the disease resistance to citrus canker. The citrus CsGRAS9 gene can be specifically recognized by the TALE effector protein of citrus canker pathogen, and the promoter region of the citrus CsGRAS9 gene contains an EBE sequence bound by the pathogenic effector PthA4 of citrus canker pathogen. The present invention provides a method for enhancing the resistance to citrus canker by inducing the expression of the citrus CsGRAS9 gene. By inoculating the xanthomonas tal-free strain containing the dGRAS9 expression vector, the overexpression of the GRAS9 gene in citrus leaves can significantly reduce the incidence of citrus canker. The artificially synthesized TALE-like protein dGRAS9 of the present invention can bind to the CsGRAS9 gene promoter sequence other than EBE PthA4 and significantly reduce the bacterial growth of citrus canker pathogen in the inoculated area and improve the disease resistance to canker by overexpressing CsGRAS9 in grapefruit leaves. The function of CsGRAS9 in regulating the susceptibility to citrus canker is verified, which has great application value in the breeding of citrus canker

The invention belongs to the technical field of plant disease control, and particularly relates to an agricultural composition of paenibacillus polymyxa and gallnut extract and application. According to the invention, it is found for the first time that the gallnut extract has an inhibition effect on the growth of four different pathogenic bacteria, namely pseudomonas syringae pv. Syringae, pseudomonas syringae pv. Syringae, pear fire blight bacteria and xanthomonas citrus subspecies, and it is found that the gallnut extract has a synergistic effect on paenibacillus polymyxa; the invention also provides an agricultural composition composed of the gallnut extract and paenibacillus polymyxa, and the agricultural composition has an inhibition effect on the growth of pseudomonas syringae pv. Syringae, pseudomonas syringae pv. Syringae, pear fire blight bacteria and xanthomonas citrus subspecies, and can be applied to the field of plant disease control.