The invention discloses a quinoxaline-containing chalcone derivative and a preparation method and application thereof. The general formula of the quinoxaline-containing chalcone derivative is shown inthe description, wherein X represents 2-O or 4-O, and R1 refers to a phenyl group, a heterocyclic group and one or more substituted phenyl groups with halogen at the 2nd-6th positions, C1-C6 alkyl atthe 2nd-6th positions, C1-C6 alkoxy at the 2nd-6th positions, nitro at the 2nd-6th positions, amino at the 2nd-6th positions and trifluoromethyl at the 2nd-6th positions; R2 represents one or more hydrogen atoms, C1-C6 alkoxy groups, nitro groups, C1-C6 alkyl groups and trifluoromethyl groups or halogen atoms at the 5th position, 6th position and 7th position or 8th position in a quinoxaline structure. The derivative can inhibit xanthomonas oryzae, xanthomonas citri and ralstonia solanacearum, raw materials are easily obtained, the reaction condition is mild, the post-treatment is simple, andthe yield is high.