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Application of cinnamylic acid compound in preventing and/or treating plant diseases

A phenylacrylic acid, plant disease technology, applied in the direction of application, plant growth regulator, botany equipment and methods, etc., to achieve the effect of reducing the occurrence of drug resistance, broad application prospects, and delaying the occurrence of drug resistance

Active Publication Date: 2019-03-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional antibiotics usually target the key factors of bacterial survival, leading to the emergence of bacterial resistance is becoming more and more common and serious

Method used

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  • Application of cinnamylic acid compound in preventing and/or treating plant diseases
  • Application of cinnamylic acid compound in preventing and/or treating plant diseases
  • Application of cinnamylic acid compound in preventing and/or treating plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Test compounds CZ-4 and CZ-9 in the T3SS system hpa1 repression of gene promoters

[0036] The mutant strain ( hpa1 inPXO99 A ) on the PSA plate for activation, pick a single colony after 2 to 3 days and place it in M210 liquid medium, add cephalosporin and ampicillin, and grow to OD 600 About 2.0, transfer to fresh M210 medium according to 1:100, grow to OD 600 About 0.6, collect the bacteria by centrifugation, wash the bacteria once in XOM2 medium, then resuspend in XOM2 medium, add cephalosporin and ampicillin to adjust the OD 600 to 0.3, add the compound to be tested to a final concentration of 200 μM, use an equal volume of DMSO as a solvent control, treat at 28 °C and 200 r / min for 15 h, repeat each treatment 3 times, collect the cells by centrifugation and use 0.01 mol / L PBS (pH=7.4) buffer to resuspend the cells and adjust the OD 600 About 0.1, the mean fluorescence intensity (MFI) of GFP detected by flow cytometry.

[0037] The experimenta...

Embodiment 2

[0042] Embodiment 2: Test compound CZ-4 and CZ-9 to wild-type bacterial strain PXO99 A growth effects

[0043] The test process is: PXO99 in the presence of the test compound in rich medium M210 and T3SS induction medium XOM2 respectively. A growth curve. Since XOM2 is a poor medium, PXO99 A There was almost no growth in it, so 0.5% sucrose was supplemented as a carbon source. Will xoo Wild-type strain PXO99 A Streak on the PSA plate for activation, pick a single colony after 2 to 3 days and place it in M210 liquid medium, add cephalosporin, grow to OD 600 About 2.0, collect the cells by centrifugation, wash the cells once with sterile water, and then resuspend them in M210 or XOM2 to adjust the OD 600 is 0.1. 200 μM of the test compound was added, placed on a 96-well plate, and an equal volume of DMSO was used as a solvent control, and 3 replicates were set for each treatment. Set the temperature at 28°C, and measure it with a fully automatic growth curve instrum...

Embodiment 3

[0046] Example 3: Compounds CZ-4 and CZ-9 on PXO99 A Effects of Hypersensitivity Response (HR) on Tobacco

[0047] PXO99 A Strains were cultured in M210 medium to OD 600 About 2.0, collect the cells by centrifugation, resuspend the cells in sterile water, and adjust the OD 600 To 0.8, three compounds to be tested were added, and an equal volume of DMSO was used as a control, and treated at 28 °C for 2 h. The treated PXO99 was then injected with a needleless syringe A The strain was inoculated on Nicotiana benthamiana cultivated for two months. After the inoculation, the tobacco continued to be cultured in the greenhouse, and photographed to observe the phenomenon after 24 hours.

[0048] The effect of compounds CZ-4 and CZ-9 on PXO99 A For results on the effect of HR on tobacco see figure 2 . It can be seen from the figure that the treatment of compounds CZ-4 and CZ-9 both have a positive effect on PXO99 A HR was significantly suppressed in the non-host plant tob...

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Abstract

The invention discloses an application of cinnamylic acid compound in preventing and / or treating plant diseases. The compound of the invention can strongly inhibit the virulence factors of the plant pathogen without affecting the growth of the plant pathogen, and significantly reduce the pathogenicity of the plant pathogen, that is, without affecting the growth of the plant pathogen, the prevention and / or treating of the plant diseases caused by plant pathogens can be achieved; the compound can be used as a plant pathogen type-III secretion system inhibitor or as a drug for controlling the related plant diseases for preventing and / or treating plant diseases, also has the effect of reducing and delaying the resistance of the plant pathogen to the compound, has a long effective use period inthe prevention and / or treatment of plant diseases, and has a broad application prospect.

Description

technical field [0001] The present invention relates to the technical field of plant disease prevention and control, and more specifically relates to the application of phenylacrylic acid compounds in the prevention and / or treatment of plant diseases. Background technique [0002] Xanthomonas oryzae ( Xanthomonas oryzae pv. oryzae, Xoo ) and bacterial streak ( Xanthomonas oryzae PV . oryzicola, Xoc ) cause rice bacterial blight and streak disease respectively, which are important bacterial diseases of rice. The development of new and effective disease control drugs is one of the key problems that need to be solved urgently. Bacterial type III secretion system (T3SS) is a key virulence factor in Gram-negative pathogenic bacteria and has become one of the ideal targets for the development of new drugs. T3SS is a key virulence factor of Xoo and Xoc, highly homologous and conserved in the two pathogenic variants, and can be used as a target for novel drug molecule design. ...

Claims

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Application Information

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IPC IPC(8): A01N37/38A01N37/10A01P1/00
CPCA01N37/10A01N37/38
Inventor 崔紫宁向绪稳陶辉姜珊林以诺
Owner SOUTH CHINA AGRI UNIV
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